Distribution of T-cell subpopulations in the peripheral blood of patients with erythrodermic psoriasis

1984 ◽  
Vol 277 (1) ◽  
pp. 19-23 ◽  
Author(s):  
R. Willemze ◽  
W. J. M. Damsteeg ◽  
C. J. L. M. Meijer
Keyword(s):  
T Cell ◽  
Peripheral Blood ◽  
Erythrodermic Psoriasis ◽  
Cell Subpopulations ◽  
T Cell Subpopulations

Imbalance of T cell subpopulations in peripheral blood of children with non-Hodgkin malignant lymphoma

1980 ◽  
Vol 8 (2) ◽  
pp. 185-189 ◽  
Author(s):  
J. D. Beck ◽  
N. Wollner ◽  
D. R. Miller ◽  
R. A. Good ◽  
S. Gupta
Keyword(s):  
T Cell ◽  
Malignant Lymphoma ◽  
Peripheral Blood ◽  
Cell Subpopulations ◽  
T Cell Subpopulations

Probing the Impact of AMD3100/GCSF Mobilization on the Peripheral Blood T Cell Content Using a Rhesus Macaque Model: Increased Proportions of FoxP3+/CD4+ T Cells Occur After Mobilization and Leukapheresis

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 350-350
Author(s):  
Leslie Kean ◽  
Sharon Sen ◽  
Mark E Metzger ◽  
Aylin Bonifacino ◽  
Karnail Singh ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Peripheral Blood ◽  
Cd4 T Cells ◽  
Cell Populations ◽  
Macaque Model ◽  
Cd34 Cells ◽  
Cell Subpopulations ◽  
T Cell Subpopulations ◽  
The Impact

Abstract Abstract 350 Introduction: Leukapheresis is a widely utilized modality for collecting hematopoietic stem cells (HSCs). While collection of CD34+ cells with stem-cell activity is the primary goal of most mobilization and leukapheresis procedures, these cells only represent ∼1% of most leukapheresis products. The profile of the non-CD34+ cells is likely influenced by the choice of mobilization strategy, and has the potential to profoundly impact the post-transplant immune milieu of the transplant recipient. Two of the most critical of the CD34-negative cell populations that are collected during leukapheresis include effector and regulatory T cells. Thus, in evaluating mobilization regimens, the impact on these regimens on the mobilization of each of these T cell populations into the peripheral blood should be rigorously evaluated. Methods: We used a rhesus macaque model to determine the impact that mobilization with AMD3100 (a.k.a., Plerixafor or Mozobil®)+ G-CSF (“A+G”) had on peripheral blood CD4+ and CD8+ effector T cell populations as well as on FoxP3+/CD4+ T cells. Three rhesus macaques were mobilized with 10ug/kg SQ of G-CSF for five consecutive days prior to leukapheresis. AMD3100 was administered at 1mg/kg SQ in combination with the last dose of G-CSF two hours prior to leukapheresis. Leukapheresis procedures were performed for two hours using a modified CS3000 Plus cell separator. A peripheral blood sample was taken before cytokine therapy, just prior to leukapheresis following mobilization, one hour during leukapheresis, and at the end of the procedure. These samples were analyzed by multicolor flow cytometry using a BD LSRII flow cytometer. Results: Bulk, effector, and regulatory T cell subpopulations were analyzed flow cytometrically. The proportion of total CD3+ T cells remained stable during mobilization and apheresis: Thus, CD3+ T cells represented 77% of peripheral blood lymphocytes prior to mobilization, and 69% post-apheresis). The balance of CD4+ to CD8+ T cells was also relatively stable. Thus, for one of the three animals tested, the CD4+ and CD8+ proportions remained unchanged after apheresis. For two animals, the average CD4+ % decreased from 67% prior to mobilization to 52% post-apheresis. In these two animals, there was a reciprocal increase in the % of CD3+ T cells that were CD8+ (28% pre-G+A to 40% post-apheresis). The CD28+/CD95- naïve (Tn), CD28+/CD95+ central memory (Tcm) and CD28-/CD95+ effector memory (Tem) subpopulation balance of CD4+ and CD8+ T cells was also determined, by comparing the relative percentages of each subpopulation post-apheresis with their relative percentages prior to mobilization. Compared to their pre-G+A percentages, the post-apheresis CD4+ percentages of Tn, Tcm and Tem were 92%, 93% and 160%, respectively. Thus, the relative proportions of Tn and Tcm CD4+ cells decreased post-apheresis, while the relative proportion of CD4+ Tem increased compared to cytokine administration. For CD8+ T cell subpopulations, the post-apheresis proportions of Tn, Tcm, and Tem compared to their pre-G-CSF proportions were 99%, 70% and 130%, respectively–thus demonstrating the same direction of change as observed for CD4+ T cells. The most striking change in T cell subpopulations occurred in the CD4+/FoxP3+ compartment. The proportion of CD4+ T cells expressing FoxP3 increased by an average of 600% when post-apheresis samples were compared to pre-mobilization samples (FoxP3+ cells were 9.6% of CD4+ T cells post-apheresis versus 1.5% pre-GCSF). An average of 32% of these FoxP3+ CD4+ T cells expressed high levels of CXCR4. CXCR4 expression has been previously documented on human FoxP3+ T cells (Zou et al., Cancer Res, 2004), but this is the first observation of high level expression of CXCR4 on macaque FoxP3+ CD4 T cells, or of their ability to be efficiently mobilized with AMD3100. Discussion: These results suggest that treatment with AMD3100 and G-CSF may mobilize T cell subsets into the peripheral blood that could have beneficial effects during allo-transplantation. The combination of an increase in Tem cells, which have been observed to have decreased ability to cause GvHD (Zheng et al., Blood 2008), along with FoxP3+/CD4+ T cells, which may have regulatory functions, suggests that A+G mobilization could produce an apheresis product with a beneficial CD34-negative cell profile for allogeneic transplantation. Disclosures: No relevant conflicts of interest to declare.

Analysis of FOXP3+ regulatory T cell subpopulations in peripheral blood and tissue of patients with systemic lupus erythematosus

2017 ◽  
Vol 65 (2) ◽  
pp. 551-563 ◽  
Author(s):  
Angelika Schmidt ◽  
Cosima C. Rieger ◽  
Ram Kumar Venigalla ◽  
Szabolcs Éliás ◽  
Regina Max ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
T Cell ◽  
Lupus Erythematosus ◽  
Regulatory T Cell ◽  
Peripheral Blood ◽  
Systemic Lupus ◽  
Cell Subpopulations ◽  
T Cell Subpopulations

Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood

2018 ◽  
Vol 199 ◽  
pp. 22-31 ◽  
Author(s):  
Joy E. Tomlinson ◽  
Bettina Wagner ◽  
M. Julia B. Felippe ◽  
Gerlinde R. Van de Walle
Keyword(s):  
T Cell ◽  
Cell Sorting ◽  
Peripheral Blood ◽  
Fluorescence Activated Cell Sorting ◽  
Cell Subpopulations ◽  
T Cell Subpopulations

Retrospective study correlating clinical infectious history and peripheral blood T-cell subpopulations in cancer, GvH and HIV+ patients

1992 ◽  
Vol 46 (1) ◽  
pp. 17-19 ◽  
Author(s):  
G Mathé ◽  
P Meyer ◽  
S Brienza ◽  
M Gil-Delgado ◽  
S Orbach-Arbouys ◽  
...  
Keyword(s):  
Retrospective Study ◽  
T Cell ◽  
Peripheral Blood ◽  
Hiv Patients ◽  
Cell Subpopulations ◽  
T Cell Subpopulations

scholarly journals Characterization of T-cell Subpopulations in Patients with Chronic Rhinosinusitis with Nasal Polyposis

2017 ◽  
Vol 8 (3) ◽  
pp. ar.2017.8.0214 ◽  
Author(s):  
Pascal Ickrath ◽  
Norbert Kleinsasser ◽  
Xin Ding ◽  
Christian Ginzkey ◽  
Niklas Beyersdorf ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Peripheral Blood ◽  
Nasal Polyps ◽  
Nasal Polyposis ◽  
Effector Memory ◽  
Forkhead Box ◽  
Cell Subpopulations ◽  
T Cell Subpopulations

Background There is an ongoing discussion concerning the potential origins of chronic rhinosinusitis with nasal polyposis (CRSwNP). Objective The aim of this study was to quantify subpopulations of T cells in peripheral blood and nasal polyps in CRSwNP to examine their influence on the etiology of this disease. Methods Tissue and blood samples were collected from 11 patients who underwent nasal sinus surgery, and these samples were analyzed by multicolor flow cytometry. Results There was a significantly lower frequency of CD4+ T-helper (Th) cells and a significantly higher frequency of CD8+ T cells among lymphocytes isolated from nasal polyps compared with peripheral blood mononuclear cells (PBMC). In both T-cell subpopulations, a shift mainly from naive T cells among peripheral blood lymphocytes toward an effector memory and terminally differentiated subtype predominance in nasal polyps was observed. Among CD4+ T cells, the frequencies of cluster of differentiation (CD) 45RA- Forkhead-Box-Protein P3high (FoxP3high) cytotoxic T-lymphocyte-associated Protein 4high (CTLA-4high) activated regulatory T (Treg) cells, and CD45RA- Forkhead-Box-Protein P3low (FoxP3low) memory T cells were significantly increased in nasal polyps compared with PBMC. Conclusion In this study, we presented a detailed characterization of CD4+ and CD8+ T-cell subpopulations in patients with CRSwNP. CD8+ T cells were more prominent in nasal polyps than in CD4+ T cells. Both nasal CD8+ T cells and CD4+ T cells predominantly had an effector memory phenotype. Among CD4+ T cells, activated Treg cells were increased in nasal polyps compared with PBMC. The data point toward a local regulation of T-cell composition within the microenvironment of nasal polyps, which might be further exploited in the future to develop novel immunotherapeutic strategies.

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