Calcium uptake in skeletal muscle mitochondria

Charlotte A. Tate; Hugh W. Bonner; Steven W. Leslie

doi:10.1007/bf00421715

Calcium uptake and release by skeletal-muscle mitochondria

Cell Calcium ◽

10.1016/0143-4160(80)90025-1 ◽

1980 ◽

Vol 1 (2) ◽

pp. 119-128 ◽

Cited By ~ 9

Author(s):

James R. Mickelson ◽

B.Bruce Marsh

Keyword(s):

Skeletal Muscle ◽

Calcium Uptake ◽

Muscle Mitochondria ◽

Skeletal Muscle Mitochondria ◽

Uptake And Release

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The Effect of Sodium and Potassium on Calcium Uptake by Frog Skeletal Muscle Mitochondria and Vesicles

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y72-169 ◽

1972 ◽

Vol 50 (12) ◽

pp. 1157-1161 ◽

Cited By ~ 5

Author(s):

Satish Batra

Keyword(s):

Skeletal Muscle ◽

Calcium Uptake ◽

Frog Skeletal Muscle ◽

Muscle Mitochondria ◽

Skeletal Muscle Mitochondria ◽

Sucrose Medium ◽

Ca Uptake ◽

Sodium And Potassium

Calcium uptake by mitochondria of frog skeletal muscle increased when K replaced Na or sucrose in the medium. There was no difference in passive binding of Ca and the amount of ATP split when K replaced Na. Ca uptake was increased by substituting K for Na in the medium and was maximal in a medium containing 100 mM K and no Na. Azide and dinitrophenol (DNP) inhibited K-stimulated Ca uptake completely. Inhibition by these agents in Na medium was relatively small. Ca uptake by vesicles was little affected by changing from Na to K medium, by azide, or by DNP. Ageing reduced Ca uptake in both fractions and stimulation by K of mitochondrial Ca uptake nearly disappeared after 4 h of storage (at 4 °C). Since Ca was taken up to the same extent in Na or sucrose medium, it is concluded that Ca uptake by mitochondria is stimulated by K rather than inhibited by Na.

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Functional Changes Induced by Short Term Caloric Restriction in Cardiac and Skeletal Muscle Mitochondria

10.1101/2020.03.13.991232 ◽

2020 ◽

Author(s):

Julian David C. Serna ◽

Camille C. Caldeira da Silva ◽

Alicia J. Kowaltowski

Keyword(s):

Skeletal Muscle ◽

Hydrogen Peroxide ◽

Caloric Restriction ◽

Calcium Uptake ◽

Atp Synthesis ◽

Calcium Handling ◽

Muscle Mitochondria ◽

Skeletal Muscle Mitochondria ◽

Uptake Rates ◽

Hydrogen Peroxide Release

AbstractCaloric restriction (CR) is widely known to increase life span and resistance against different types of injuries in several organisms. We have previously shown that mitochondria from livers or brains of CR animals exhibit higher calcium uptake rates and lower sensitivity to calcium-induced mitochondrial permeability transition (mPT), an event related to the resilient phenotype exhibited by these organs. Given the importance of calcium in metabolic control and cell homeostasis, we aimed here to uncover possible changes in mitochondrial calcium handling, redox balance and bioenergetics in cardiac and skeletal muscle mitochondria. Unexpectedly, we found that CR does not alter the susceptibility to mPT in muscle (cardiac or skeletal), nor calcium uptake rates. Despite the lack in changes in calcium transport properties, CR consistently decreased respiration in the presence of ATP synthesis in heart and soleus muscle. In heart, such changes were accompanied by a decrease in respiration in the absence of ATP synthesis, lower maximal respiratory rates and a reduced rate of hydrogen peroxide release. Hydrogen peroxide release was unaltered by CR in skeletal muscle. No changes were observed in inner membrane potentials and respiratory control ratios. Together, these results highlight the tissue-specific bioenergetic and ion transport effects induced by CR, demonstrating that resilience against calcium-induced mPT is not present in all tissues.

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Calcium uptake and retention by skeletal-muscle mitochondria

Biochemical Society Transactions ◽

10.1042/bst0120480 ◽

1984 ◽

Vol 12 (3) ◽

pp. 480-481

Author(s):

ASHLEY P. ALLSHIRE ◽

JAMES J. A. HEFFRON

Keyword(s):

Skeletal Muscle ◽

Calcium Uptake ◽

Muscle Mitochondria ◽

Skeletal Muscle Mitochondria

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Increased calcium uptake by muscle mitochondria of cold-acclimated rats

AJP Cell Physiology ◽

10.1152/ajpcell.1978.234.1.c7 ◽

1978 ◽

Vol 234 (1) ◽

pp. C7-C13 ◽

Cited By ~ 22

Author(s):

D. C. Greenway ◽

J. Himms-Hagen

Keyword(s):

Skeletal Muscle ◽

Calcium Transport ◽

Prostaglandin E1 ◽

Calcium Uptake ◽

Mitochondrial Calcium ◽

Nonshivering Thermogenesis ◽

Spontaneous Release ◽

Muscle Mitochondria ◽

Skeletal Muscle Mitochondria ◽

Transport Cycle

Skeletal muscle mitochondria of cold-acclimated rats have an altered morphology that is related to the occurrence of nonshivering thermogenesis. The transport of calcium by these mitochondria was studied in a search for an alteration in an energy-dissipating mechanism which might be related to the altered morphology and to the altered mode of thermogenesis in the cold-acclimated animal. The rates of calcium uptake, of calcium-stimulated respiration, and of state 4 respiration after calcium uptake were increased in the altered mitochondria. The capacity to accumulate calcium without phosphate was increased, whereas with phosphate all the calcium was removed from the medium and no difference in total uptake was seen. Spontaneous release of calcium was greater but sodium-induced release was unchanged. No effect of cyclic AMP or prostaglandin E1 on release of calcium was seen. The increase in rate of calcium uptake occurred gradually during the first 3-5 wk of acclimation to cold. The results are considered to give some support to the hypothesis that adaptive changes in the mitochondrial calcium transport cycle in skeletal muscle occur during acclimation to cold.

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