Identification of aggregation substances of Enterococcus faecalis cells after induction by sex pheromones

1989 ◽  
Vol 151 (6) ◽  
pp. 486-490 ◽  
Author(s):  
Dominique Galli ◽  
Reinhard Wirth ◽  
Gerhard Wanner
Keyword(s):  
Enterococcus Faecalis ◽  
Sex Pheromones

scholarly journals Sex pheromones and plasmid transfer in Enterococcus faecalis

Plasmid ◽  
1989 ◽  
Vol 21 (3) ◽  
pp. 175-184 ◽  
Author(s):  
Don B. Clewell ◽  
Keith E. Weaver
Keyword(s):  
Enterococcus Faecalis ◽  
Sex Pheromones ◽  
Plasmid Transfer

scholarly journals Characterization of a class of nonformylated Enterococcus faecalis-derived neutrophil chemotactic peptides: the sex pheromones.

1990 ◽  
Vol 87 (1) ◽  
pp. 66-70 ◽  
Author(s):  
P. Sannomiya ◽  
R. A. Craig ◽  
D. B. Clewell ◽  
A. Suzuki ◽  
M. Fujino ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Sex Pheromones ◽  
Chemotactic Peptides

scholarly journals An ABC Transporter Is Required for Secretion of Peptide Sex Pheromones in Enterococcus faecalis

mBio ◽  
2014 ◽  
Vol 5 (5) ◽  
Author(s):  
Sriram Varahan ◽  
Nathan Harms ◽  
Michael S. Gilmore ◽  
John M. Tomich ◽  
Lynn E. Hancock
Keyword(s):  
Enterococcus Faecalis ◽  
Abc Transporter ◽  
Sex Pheromones ◽  
Cell Communication ◽  
Antibiotic Resistance Genes ◽  
The United States ◽  
Peptide Transporter ◽  
Bacterial Killing ◽  
Content Type ◽  
Biofilm Development

ABSTRACTEnterococci are leading causes of hospital-acquired infection in the United States and continue to develop resistances to new antibiotics. ManyEnterococcus faecalisisolates harbor pheromone-responsive plasmids that mediate horizontal transfer of even large blocks of chromosomal genes, resulting in hospital-adapted strains over a quarter of whose genomes consist of mobile elements. Pheromones to which the donor cells respond derive from lipoprotein signal peptides. Using a novel bacterial killing assay dependent on the presence of sex pheromones, we screened a transposon mutant library for functions that relate to the production and/or activity of the effector pheromone. Here we describe a previously uncharacterized, but well-conserved, ABC transporter that contributes to pheromone production. Using three distinct pheromone-dependent mating systems, we show that mutants defective in expressing this transporter display a 5- to 6-order-of-magnitude reduction in conjugation efficiency. In addition, we demonstrate that the ABC transporter mutant displays an altered biofilm architecture, with a significant reduction in biofilm biomass compared to that of its isogenic parent, suggesting that pheromone activity also influences biofilm development. The conservation of this peptide transporter across theFirmicutessuggests that it may also play an important role in cell-cell communication in other species within this important phylum.IMPORTANCEEnterococcus faecalisranks as one of the leading causes of hospital-associated infections. Strains possessing resistance to multiple antibiotics are becoming all too common in clinical settings. Pheromone-responsive plasmids play an important role in harboring and disseminating these antibiotic resistance genes. Here we have identified a novel ABC transporter that is responsible for the secretion of peptide pheromones, which enables communication between cells to mediate plasmid transfer. We have also shown that this transporter is important for biofilm formation, providing a strong rationale for its use as a viable therapeutic target which could be targeted to curb infection, as well as the spread of existing drug resistance.

Purification of sex pheromones specific for pMB1 and pMB2 plasmids of Enterococcus faecalis S-48

1995 ◽  
Vol 41 (7) ◽  
pp. 629-632 ◽  
Author(s):  
R. Quirantes ◽  
E. Valdivia ◽  
I. Martín ◽  
M. Martínez-Bueno ◽  
M. Maqueda ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Type Strain ◽  
Sex Pheromones ◽  
Wild Type Strain ◽  
Reversed Phase ◽  
Wild Type ◽  
Pheromone Response ◽  
Conjugative Plasmids ◽  
In The Wild ◽  
Key Words Pheromone

The strain Enterococcus faecalis S-48 carries two large conjugative plasmids (pMB1 and pMB2) encoding for antagonistic substances. The pheromone response of these two plasmids was established by purifying the corresponding sex pheromones, using conventional reversed-phase columns. Plasmid pMB1 responds to pheromone cCF10. Plasmid pMB2 responds to a pheromone with an amino acid sequence identical to that of cPD1 (Phe-Leu-Val-Met-Phe-Leu-Ser-Gly). The two pheromone-responding plasmids coexist in a stable fashion in the wild-type strain E. faecalis S-48.Key words: pheromone response, Enterococcus faecalis, conjugative plasmids.

Localization of aggregation substances of Enterococcus faecalis after induction by sex pheromones

1989 ◽  
Vol 151 (6) ◽  
pp. 491-497 ◽  
Author(s):  
Gerhard Wanner ◽  
Helmut Formanek ◽  
Dominique Galli ◽  
Reinhard Wirth
Keyword(s):  
Enterococcus Faecalis ◽  
Sex Pheromones

Wrażliwość klinicznych szczepów Enterococcus faecalis wywołujących zakażenia miejscowe w formie biofilmowej i planktonicznej na antyseptyki

2017 ◽  
Vol 8 (5) ◽  
pp. 337-343
Author(s):  
Marzenna Bartoszewicz ◽  
Adam Junka ◽  
Przemysław Dalkowski ◽  
Maciej Sopata
Keyword(s):  
Enterococcus Faecalis
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