Detection of the vitamin a carrier plasma proteins in human epidermis

1977 ◽  
Vol 258 (1) ◽  
pp. 85-88 ◽  
Author(s):  
Urban Forsum ◽  
Lars Rask ◽  
Ulla Malmn�s Tjernlund ◽  
Per A. Peterson
Keyword(s):  
Vitamin A ◽  
Plasma Proteins ◽  
Human Epidermis

scholarly journals The early changes in retinol-binding protein and prealbumin concentrations in plasma of protein–energy malnourished children after treatment with retinol and an improved diet

1980 ◽  
Vol 43 (3) ◽  
pp. 393-402 ◽  
Author(s):  
Suzanne Large ◽  
G. Neal ◽  
J. Glover ◽  
O. Thanangkul ◽  
R. E. Olson
Keyword(s):  
Body Weight ◽  
Vitamin A ◽  
Nutritional Status ◽  
Plasma Proteins ◽  
Binding Protein ◽  
Vitamin A Deficiency ◽  
Plasma Concentrations ◽  
Retinol Binding Protein ◽  
Mean Values ◽  
The Mean

1. Changes in total retinol-binding protein (RBP), the holoprotein (holoRBP) and prealbumin (PA) concentrations have been monitored in plasma of thirty protein- and vitamin A-deficient preschool children from within a few hours up to 7 weeks after treatment with retinol and a good-quality protein diet.2. The children were classified into groups according to nutritional status as having either kwashiorkor, marasmus-kwashiorkor or marasmus, and given formula diets whose protein and energy contents increased stepwise from 1 g and 105 kJ/kg body-weight respectively up to 4 g and 733 kJ/kg body-weight after 4 weeks. Retinol was administered in the forms of retinyl palmitate either orally or intramuscularly.3. PA and total RBP were determined by electroimmunoassay procedures and the holoRBP by its fluorescence after separation from other plasma proteins.4. RBP in plasma of the vitamin A-deficient child is largely denatured and incapable of binding administered retinol, which must first be taken up by the liver before native holoRBP is released. An increased pool of native apoprotein accumulates in the liver during vitamin A deficiency which is released into plasma quickly after retinol uptake to form peak concentrations of total and holoRBP approximately 3 h after dosing intramuscularly and 6 h orally.5. The accumulated pool of RBP was highest in livers from the marasmus group and lowest in those from the kwashiorkor group, reflecting their relative capacities to synthesize plasma proteins.6. The mean plasma concentrations of total and holoRBP for the various groups were minimal 24–48 h after dosing with retinol and then improved almost linearly over the following week.7. Mean plasma PA concentrations of the various groups on admission were also in order of the severity of their malnutrition. There was little or no change in this protein concentration over the first 24 h after dosing with retinol, but thereafter the mean values rose almost linearly over 2 weeks. Albumin on the other hand changed little during the first week. The results show that PA is the more sensitive measurement of protein nutritional status.

Vitamin A transporting proteins in human epidermis and blister fluids

1983 ◽  
Vol 275 (5) ◽  
pp. 324-328 ◽  
Author(s):  
H. T�rm� ◽  
A. Vahlquist
Keyword(s):  
Vitamin A ◽  
Human Epidermis

Vitamin A transporting plasma proteins and female sex hormones

1979 ◽  
Vol 32 (7) ◽  
pp. 1433-1438 ◽  
Author(s):  
A Vahlquist ◽  
A Johnsson ◽  
K-G Nygren
Keyword(s):  
Vitamin A ◽  
Sex Hormones ◽  
Plasma Proteins ◽  
Female Sex ◽  
Female Sex Hormones

scholarly journals Biometric Analysis of the Effect of Oral Vitamin A on Human Epidermis

1964 ◽  
Vol 42 (1) ◽  
pp. 131-136 ◽  
Author(s):  
Hermann Pinkus ◽  
Rosie Hunter
Keyword(s):  
Vitamin A ◽  
Human Epidermis ◽  
Oral Vitamin ◽  
Biometric Analysis

Studies of the distribution of vitamin A as ester and alcohol and of carotenoids in plasma proteins of several species

1952 ◽  
Vol 38 (1) ◽  
pp. 275-282 ◽  
Author(s):  
J. Ganguly ◽  
N.I. Krinsky ◽  
John W. Mehl ◽  
H.J. Deuel
Keyword(s):  
Vitamin A ◽  
Plasma Proteins

Immunoenzymatic demonstration of the simultaneous presence of transferrin, hemopexin and albumin in a same hepatocyte and their ultrastructural localization

1978 ◽  
Vol 36 (2) ◽  
pp. 88-89
Author(s):  
M. Kraemer ◽  
J. Foucrier ◽  
J. Vassy ◽  
M.T. Chalumeau
Keyword(s):  
Plasma Proteins ◽  
Rat Hepatocytes ◽  
Ultrastructural Localization ◽  
Carrier Proteins ◽  
Normal Cells ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Monospecific Antisera ◽  
Different Levels

Some authors using immunofluorescent techniques had already suggested that some hepatocytes are able to synthetize several plasma proteins. In vitro studies on normal cells or on cells issued of murine hepatomas raise the same conclusion. These works could be indications of an hepatocyte functionnal non-specialization, meanwhile the authors never give direct topographic proofs suitable with this hypothesis.The use of immunoenzymatic techniques after obtention of monospecific antisera had seemed to us useful to bring forward a better knowledge of this problem. We have studied three carrier proteins (transferrin = Tf, hemopexin = Hx, albumin = Alb) operating at different levels in iron metabolism by demonstrating and localizing the adult rat hepatocytes involved in their synthesis.Immunological, histological and ultrastructural methods have been described in a previous work.

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