Coupled sodium and chloride transport into plasma membrane vesicles prepared from dogfish rectal gland

1978 ◽  
Vol 378 (2) ◽  
pp. 87-92 ◽  
Author(s):  
Jill Eveloff ◽  
Rolf Kinne ◽  
Eva Kinne-Saffran ◽  
Heini Murer ◽  
Patricio Silva ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Chloride Transport ◽  
Membrane Vesicles ◽  
Rectal Gland ◽  
Plasma Membrane Vesicles

The iodide channel of the thyroid: a plasma membrane vesicle study

1992 ◽  
Vol 263 (3) ◽  
pp. C590-C597 ◽  
Author(s):  
P. Golstein ◽  
M. Abramow ◽  
J. E. Dumont ◽  
R. Beauwens
Keyword(s):  
Plasma Membrane ◽  
Chloride Transport ◽  
Membrane Vesicle ◽  
Membrane Vesicles ◽  
Anion Channel ◽  
Apical Plasma Membrane ◽  
Plasma Membrane Vesicle ◽  
Plasma Membrane Vesicles ◽  
Bovine Thyroid ◽  
Set Up

The uptake of radioactive iodide or chloride by plasma membrane vesicles of bovine thyroid was studied by a rapid filtration technique. A Na(+)-I- cotransport was demonstrated. When this Na(+)-I- cotransport is inactive (i.e., at 4 degrees C and in the absence of Na+), an uptake of iodide above chemical equilibrium could be induced, driven by the membrane potential. The latter was set up by allowing potassium to diffuse into the membrane vesicles in the presence of valinomycin and of an inward K+ gradient. This potential difference (positive inside) induced the uptake of iodide (or other anion present). The data support the existence of two anionic channels. The first one, observed at low near-physiological iodide concentration (micromolar range), which exhibits a high permeability and specificity for iodide (hence called the iodide channel), has a Km of 70 microM. The other one appears similar to the epithelial anion channel as described by Landry et al. (J. Gen. Physiol. 90: 779-798, 1987); it is still about fourfold more permeable to iodide than to chloride and presents a Km of 33 mM. Under physiological conditions the latter channel would mediate chloride transport, and the iodide channel, which is proposed to be restricted to the apical plasma membrane domain of the thyrocyte, transports iodide from the cytosol to the colloid space.

scholarly journals Ca2+-ATPase-driven calcium accumulation in Ustilago maydis plasma membrane vesicles

Microbiology ◽  
1994 ◽  
Vol 140 (11) ◽  
pp. 3047-3051 ◽  
Author(s):  
A. Hernandez ◽  
D. T. Cooke ◽  
D. T. Clarkson
Keyword(s):  
Plasma Membrane ◽  
Membrane Vesicles ◽  
Ustilago Maydis ◽  
Plasma Membrane Vesicles ◽  
Calcium Accumulation
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