Chromosomal homologies betweenDrosophila lebanonensis andD. melanogaster determined by in situ hybridization

Chromosoma ◽  
1993 ◽  
Vol 102 (5) ◽  
pp. 361-368 ◽  
Author(s):  
M. Papaceit ◽  
E. Juan
Keyword(s):  
In Situ Hybridization ◽  
Chromosomal Homologies

The actin loci in the genus Drosophila: establishment of chromosomal homologies among distantly related species by in situ hybridization

Chromosoma ◽  
1986 ◽  
Vol 94 (4) ◽  
pp. 297-308 ◽  
Author(s):  
Michael Loukas ◽  
Fotis C. Kafatos
Keyword(s):  
In Situ Hybridization ◽  
Related Species ◽  
Chromosomal Homologies

The actin loci in the genus Drosophila : establishment of chromosomal homologies among five palearctic species of the Drosophila obscura group by in situ hybridization

Chromosoma ◽  
2001 ◽  
Vol 110 (7) ◽  
pp. 441-450 ◽  
Author(s):  
George Bondinas ◽  
Michael Loukas ◽  
George Goulielmos ◽  
Diether Sperlich
Keyword(s):  
In Situ Hybridization ◽  
Palearctic Species ◽  
Chromosomal Homologies ◽  
Obscura Group

scholarly journals In situ hybridization analysis of chromosomal homologies in Drosophila melanogaster and Drosophila virilis.

Genetics ◽  
1989 ◽  
Vol 122 (1) ◽  
pp. 99-109 ◽  
Author(s):  
J H Whiting ◽  
M D Pliley ◽  
J L Farmer ◽  
D E Jeffery
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Gland ◽  
In Situ Hybridization ◽  
Recombinant Dna ◽  
Unique Sequence ◽  
Drosophila Virilis ◽  
Multigene Families ◽  
Larval Salivary Gland ◽  
Chromosomal Homologies

Abstract Twenty-four biotin-labeled recombinant-DNA probes which contained putative unique-sequence Drosophila melanogaster DNA were hybridized to larval salivary-gland chromosomes of D. melanogaster and Drosophila virilis. All probes hybridized to D. melanogaster chromosomes at the expected sites. However, one probe hybridized to at least 16 additional sites, and one hybridized to one additional site. Thirteen probes hybridized strongly to D. virilis chromosomes, four hybridized weakly and infrequently, and seven did not hybridize. Probes representing two multigene families (beta-tubulin and yolk-protein) hybridized as would be expected if all sites had been conserved in the two species on the same chromosomal elements. The multiple hybridization sites of a third probe which may represent a multigene family were also conserved. The results were consistent with H.J. Muller's proposal that chromosomal elements have been conserved during evolution of this genus.

Fluorescence in situ hybridization (FISH) maps chromosomal homologies between the dusky titi and squirrel monkey

2000 ◽  
Vol 50 (2) ◽  
pp. 95-107 ◽  
Author(s):  
R. Stanyon ◽  
S. Consigliere ◽  
S. M�ller ◽  
A. Morescalchi ◽  
M. Neusser ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Squirrel Monkey ◽  
Chromosomal Homologies

The actin loci in the genus Drosophila: establishment of chromosomal homologies among five nearctic species of the Drosophila obscura group by in situ hybridization

Chromosoma ◽  
2002 ◽  
Vol 111 (4) ◽  
pp. 256-266 ◽  
Author(s):  
George P. Bondinas ◽  
Michael G. Loukas ◽  
George N. Goulielmos ◽  
Diether Sperlich
Keyword(s):  
In Situ Hybridization ◽  
Chromosomal Homologies ◽  
Obscura Group

scholarly journals Differentiation of Muller's Chromosomal Elements D and E in the Obscura Group of Drosophila

Genetics ◽  
1996 ◽  
Vol 144 (1) ◽  
pp. 139-146
Author(s):  
Carmen Segarra ◽  
Griselda Ribó ◽  
Montserrat Aguadé
Keyword(s):  
In Situ Hybridization ◽  
Homologous Chromosome ◽  
Genomic Library ◽  
Pairwise Comparisons ◽  
Species Divergence ◽  
Chromosomal Homologies ◽  
Obscura Group

Abstract Twenty-two markers located on Muller's elements D or E have been mapped by in situ hybridization in six species of the obscura group of Drosophila and in D. melanogaster. The obscura species can be grouped into a Palearctic cluster (D. subobscura, D. madeirensis and D. guanche) and a Nearctic one (D. pseudoobscura, D. persimilis and D. miranda). Eleven of the probes contain known genes: E74, Acp70A, Est-5, hsp28/23, hsp83, emc, hsp70, Xdh, Acph-I, Cec and rp49. The remaining probes are recombinant phages isolated from a D. subobscura genomic library. All these markers hybridize to the putative homologous chromosome or chromosomal arm of elements D and E. Thus, these elements have conserved their genic content during species divergence. Chromosomal homologies proposed previously for each element among the species of the same cluster have been compared with the present results. The distribution of markers within each element has changed considerably as inferred from pairwise comparisons of obscura species included in the two different clusters. Only chromosomal segments defined by closely linked markers have been conserved: one such segment has been detected in element D and three in element E between D. subobscura and D. pseudoobscura.

DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

1992 ◽  
Vol 50 (1) ◽  
pp. 496-497
Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Dual Band ◽  
Nick Translation ◽  
Metaphase Chromosomes ◽  
Band Pass ◽  
Texas Red ◽  
Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

Ultrastructural analysis of the spatial distribution of MRNA

1992 ◽  
Vol 50 (1) ◽  
pp. 552-553
Author(s):  
Gary Bassell ◽  
Robert H. Singer
Keyword(s):  
Electron Microscopy ◽  
Spatial Distribution ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Nucleic Acids ◽  
Colloidal Gold ◽  
Dna Probe ◽  
Nucleotide Analogs ◽  
Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

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