Isolation and characterization of the glucose-6-phosphate dehydrogenase encoding gene (gsdA) fromAspergillus niger

1995 ◽  
Vol 247 (2) ◽  
pp. 229-239 ◽  
Author(s):  
Peter van den Broek ◽  
Theo Goosen ◽  
Bert Wennekes ◽  
Henk van den Broek
Keyword(s):  
Isolation And Characterization ◽  
Encoding Gene ◽  
Glucose 6 Phosphate Dehydrogenase

scholarly journals Characterization of a Novel Fructosyltransferase from Lactobacillus reuteri That Synthesizes High-Molecular-Weight Inulin and Inulin Oligosaccharides

2002 ◽  
Vol 68 (9) ◽  
pp. 4390-4398 ◽  
Author(s):  
S. A. F. T. van Hijum ◽  
G. H. van Geel-Schutten ◽  
H. Rahaoui ◽  
M. J. E. C. van der Maarel ◽  
L. Dijkhuizen
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Lactobacillus Reuteri ◽  
Bacterial Strains ◽  
Potential Health ◽  
Isolation And Characterization ◽  
A Cell ◽  
Encoding Gene ◽  
First Time

ABSTRACT Fructosyltransferase (FTF) enzymes produce fructose polymers (fructans) from sucrose. Here, we report the isolation and characterization of an FTF-encoding gene from Lactobacillus reuteri strain 121. A C-terminally truncated version of the ftf gene was successfully expressed in Escherichia coli. When incubated with sucrose, the purified recombinant FTF enzyme produced large amounts of fructo-oligosaccharides (FOS) with β-(2→1)-linked fructosyl units, plus a high-molecular-weight fructan polymer (>107) with β-(2→1) linkages (an inulin). FOS, but not inulin, was found in supernatants of L. reuteri strain 121 cultures grown on medium containing sucrose. Bacterial inulin production has been reported for only Streptococcus mutans strains. FOS production has been reported for a few bacterial strains. This paper reports the first-time isolation and molecular characterization of (i) a Lactobacillus ftf gene, (ii) an inulosucrase associated with a generally regarded as safe bacterium, (iii) an FTF enzyme synthesizing both a high molecular weight inulin and FOS, and (iv) an FTF protein containing a cell wall-anchoring LPXTG motif. The biological relevance and potential health benefits of an inulosucrase associated with an L. reuteri strain remain to be established.

Isolation and characterization of an AP2/ERF-type drought stress inducible transcription factor encoding gene from rice

2013 ◽  
Vol 23 (1) ◽  
pp. 42-51 ◽  
Author(s):  
Ibandalin Mawlong ◽  
Kishwar Ali ◽  
Devika Kurup ◽  
Sangita Yadav ◽  
Aruna Tyagi
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Isolation And Characterization ◽  
Encoding Gene

Isolation and characterization of the rat catalase-encoding gene

Gene ◽  
1989 ◽  
Vol 79 (2) ◽  
pp. 279-288 ◽  
Author(s):  
Nakashima Hideaki ◽  
Yamamoto Mikio ◽  
Goto Kiminobu ◽  
Osumi Takashi ◽  
Hashimoto Takashi ◽  
...  
Keyword(s):  
Isolation And Characterization ◽  
Encoding Gene

Isolation and characterization of a novel interferon-α-encoding gene, IFN-α11 within a murine IFN cluster

Gene ◽  
1991 ◽  
Vol 104 (2) ◽  
pp. 187-195 ◽  
Author(s):  
Colette Coulombel ◽  
Guilane Vodjdani ◽  
Janine Doly
Keyword(s):  
Interferon Α ◽  
Isolation And Characterization ◽  
Encoding Gene

Isolation and characterization of ompS1, a novel Salmonella typhi outer membrane protein-encoding gene

Gene ◽  
1995 ◽  
Vol 158 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Marcos Fernández-Mora ◽  
Ricardo Oropeza ◽  
JoséLuis Puente ◽  
Edmundo Calva
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Salmonella Typhi ◽  
Isolation And Characterization ◽  
Protein Encoding ◽  
Encoding Gene

scholarly journals Construction of a Novel Chimeric Dextransucrase Fused to the Carbohydrate-Binding Module CBM2a

2021 ◽  
Author(s):  
Reinaldo Fraga Vidal ◽  
Roberto Carlos Aristicas Ribalta ◽  
Lisandra Teresa Martínez Valdés ◽  
Meinardo Lafargue Gámez ◽  
Amanda Montes Alvarez ◽  
...  
Keyword(s):  
Binding Capacity ◽  
Structural Characteristics ◽  
Carbohydrate Binding ◽  
Carbohydrate Binding Module ◽  
Molecular Weights ◽  
Isolation And Characterization ◽  
The Subject ◽  
Encoding Gene ◽  
First Time

The lactic acid bacteria (LAB) have great potential to produce homoexopolysaccharides (HoPS), have been the subject of extensive research efforts, given their health benefits and physicochemical properties. The HoPS functional properties are determined by structural characteristics of varied molecular weights, types of glycosidic linkages, degrees of branching and chemical composition. The dextransucrases (DSases) are responsible of the synthesis of a kind of HoPS (dextran polymers), which are among the first biopolymers produced at industrial scale with applications in medicine and biotechnology. The concept of glycodiversification opens additional applications for DSases. In that sense the design and characterization of new DSases is of prime importance. Previously, we described the isolation and characterization of a novel extracellular dextransucrase (DSR-F) encoding gene. In this study, from DSR-F, we design a novel chimeric dextransucrase DSR-F-∆SP-∆GBD-CBM2a, where DSR-F-∆SP-∆GBD is fused to the carbohydrate-binding module (CBM2a) of the β-1-4 exoglucanase/xylanase Cex (Xyn10A) of Cellulomonas fimi ATCC 484. This dextransucrase variant is active and without alteration in its specificity. The DSR-F-∆SP-∆GBD-CBM2a is purified by cellulose affinity chromatography for the very first time. Our results indicate that new hybrids and chimeric DSases with novel binding capacity to cellulose can be designed to obtain glyco-biocatalysts from renewable lignocellulosic materials.

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