Demonstration with the electron microscope of in jected peroxidase in rat liver cells

1964 ◽  
Vol 3 (6) ◽  
pp. 561-564 ◽  
Author(s):  
W. Th. Daems ◽  
M. Ploeg ◽  
J. -P. Persijn ◽  
P. Duijn
Keyword(s):  
Electron Microscope ◽  
Rat Liver ◽  
Liver Cells ◽  
Rat Liver Cells

The Cytological Basis for Cortisone Action on Rat Liver Cells

1967 ◽  
Vol 25 ◽  
pp. 18-19
Author(s):  
Jonathan E. Friedberg ◽  
Robert R. Cardell
Keyword(s):  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Rat Liver ◽  
Saline Solution ◽  
Smooth Endoplasmic Reticulum ◽  
Liver Cells ◽  
Free Access ◽  
Normal Hepatocyte ◽  
Rat Liver Cells ◽  
Cortisone Injection

The objective of this investigation is to provide morphological information on the action of a hormone. We have chosen to study the action of cortisone on the rat liver because it is established that this steroid has a stimulatory effect on the synthesis of the gluconeogenic enzymes. The glucocorticoids (including cortisone) are secreted by the adrenal cortex; hence, adrenalectomy renders the animal deficient in these hormones and impairs carbohydrate metabolism in the hepatocyte by reducing gluconeogenesis. Cortisone injection into an adrenalectomized rat increases the capacity of this cell to produce glucose from non-carbohydrate precursors. We have studied with the electron microscope liver cells from normal, adrenalectomized, and adrenalectomized, cortisone-treated rats. All rats were allowed free access to saline solution and were fasted for 15 hours before sacrifice.The liver cell of the adrenalectomized rat (Fig. 1) presents several striking alterations as compared with the normal hepatocyte. A conspicuous difference is the almost complete absence of particulate glycogen after the animal has been fasted for 15 hours. More remarkable is the very sparse development of the smooth endoplasmic reticulum (SER) in these glycogen-depleted cells.

Effects of Hypophysectomy on the Fine Structure of Rat Liver Cells

1967 ◽  
Vol 25 ◽  
pp. 156-157
Author(s):  
Robert R. Cardell
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Rat Liver ◽  
Liver Cell ◽  
Anterior Pituitary ◽  
Liver Cells ◽  
Biochemical Studies ◽  
Liver Functions ◽  
Rat Liver Cells ◽  
And Control

Hypophysectomy of the rat renders this animal deficient in the hormones of the anterior pituitary gland, thus causing many primary and secondary hormonal effects on basic liver functions. Biochemical studies of these alterations in the rat liver cell are quite extensive; however, relatively few morphological observations on such cells have been recorded. Because the available biochemical information was derived mostly from disrupted and fractionated liver cells, it seemed desirable to examine the problem with the techniques of electron microscopy in order to see what changes are apparent in the intact liver cell after hypophysectomy. Accordingly, liver cells from rats which had been hypophysectomized 5-120 days before sacrifice were studied. Sham-operated rats served as controls and both hypophysectomized and control rats were fasted 15 hours before sacrifice.

Heterogeneity of carboxylesterases in rat liver cells

1992 ◽  
Vol 44 (4) ◽  
pp. 827-829 ◽  
Author(s):  
Rolf Gaustad ◽  
Trond Berg ◽  
Frode Fonnum
Keyword(s):  
Rat Liver ◽  
Liver Cells ◽  
Rat Liver Cells
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