The effect of hyperbaric oxygen on rat liver cells in organ culture: A light- and electron-microscope study

1968 ◽  
Vol 96 (1) ◽  
pp. 149-159 ◽  
Author(s):  
R. E. Coupland ◽  
J. D. B. Macdougall
Keyword(s):  
Electron Microscope ◽  
Organ Culture ◽  
Hyperbaric Oxygen ◽  
Rat Liver ◽  
Microscope Study ◽  
Electron Microscope Study ◽  
Liver Cells ◽  
Rat Liver Cells

scholarly journals An Electron Microscope Study of Basophile Substances of Frozen-Dried Rat Liver

1958 ◽  
Vol 4 (3) ◽  
pp. 291-300 ◽  
Author(s):  
Henry Finck
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Rat Liver ◽  
Microscope Study ◽  
Electron Microscope Study ◽  
Freeze Drying ◽  
Light And Electron Microscopy ◽  
Enzyme Treatment ◽  
Dense Granules ◽  
Homogeneous Matrix

Small pieces of liver from rats subjected to different dietary regimes were fixed by freeze-drying, and postfixed by in vacuo heating and denaturation with alcohol. Specimens were digested with ribo- or deoxyribonuclease, and stained with gallocyanin-chromalum, azure II, the Feulgen procedure or alcoholic platinic tetrabromide. Some specimens were reserved as controls of the effects of enzyme treatment. Stained and unstained specimens were embedded in methacrylate and examined by light and electron microscopy. Basophilic and Feulgen-positive substances, after contact with watery reagents, were found by electron microscopy to exist as small dense granules embedded in a less dense homogeneous matrix, forming the walls of submicroscopic vacuoles. These granules were absent after digestion with nucleodepolymerases. In specimens (unstained, or stained with platinic tetrabromide) which had not passed through water, the dense (basophile) substances in nuclei and cytoplasm were found to exist, not as granules, but as ill defined submicroscopic concentrates which blended imperceptibly into the homogeneous matrix of the vacuolar walls. Objections to the use of stains for improving contrast conditions in electron microscopy of tissues are discussed, and it is concluded that the reagents do not necessarily produce the observed increases in contrast by selectively stabilizing certain structures. The concept of microsomes as pre-existing distinct morphological entities in intact (unhomogenized) cells is thought to be inconsistent with the distribution of basophile substances in frozen-dried liver.

The Cytological Basis for Cortisone Action on Rat Liver Cells

1967 ◽  
Vol 25 ◽  
pp. 18-19
Author(s):  
Jonathan E. Friedberg ◽  
Robert R. Cardell
Keyword(s):  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Rat Liver ◽  
Saline Solution ◽  
Smooth Endoplasmic Reticulum ◽  
Liver Cells ◽  
Free Access ◽  
Normal Hepatocyte ◽  
Rat Liver Cells ◽  
Cortisone Injection

The objective of this investigation is to provide morphological information on the action of a hormone. We have chosen to study the action of cortisone on the rat liver because it is established that this steroid has a stimulatory effect on the synthesis of the gluconeogenic enzymes. The glucocorticoids (including cortisone) are secreted by the adrenal cortex; hence, adrenalectomy renders the animal deficient in these hormones and impairs carbohydrate metabolism in the hepatocyte by reducing gluconeogenesis. Cortisone injection into an adrenalectomized rat increases the capacity of this cell to produce glucose from non-carbohydrate precursors. We have studied with the electron microscope liver cells from normal, adrenalectomized, and adrenalectomized, cortisone-treated rats. All rats were allowed free access to saline solution and were fasted for 15 hours before sacrifice.The liver cell of the adrenalectomized rat (Fig. 1) presents several striking alterations as compared with the normal hepatocyte. A conspicuous difference is the almost complete absence of particulate glycogen after the animal has been fasted for 15 hours. More remarkable is the very sparse development of the smooth endoplasmic reticulum (SER) in these glycogen-depleted cells.

scholarly journals An Electron Microscope Study of Intranuclear Inclusions in Mouse Liver and Hepatoma

1959 ◽  
Vol 6 (3) ◽  
pp. 427-430 ◽  
Author(s):  
Elizabeth H. Leduc ◽  
J. Walter Wilson
Keyword(s):  
Electron Microscope ◽  
Nuclear Envelope ◽  
Microscope Study ◽  
Lipid Droplets ◽  
Electron Microscope Study ◽  
Volume Ratio ◽  
Giant Cells ◽  
Liver Cells ◽  
Nuclear Surface ◽  
Intranuclear Inclusions

An electron microscope study of intranuclear inclusions which occur in giant cells in a transplantable mouse hepatoma and in enlarged liver cells in mice fed a diet containing bentonite demonstrates that these inclusions are formed by invaginations of the nuclear envelope, and corroborates a previous histochemical study which revealed that the contents of the inclusions are of cytoplasmic origin. In the hepatoma cells the intranuclear inclusions are abundant, small, and situated close to the border of the nucleus, and there are wide openings from the cytoplasm into the invaginations whose contents include lipid droplets, ergastoplasm, and structurally normal mitochondria. In the enlarged liver cells the inclusions are fewer in number, generally much larger than those in the hepatoma, hence they extend deeper into the nucleus, and the interior is continuous with the cytoplasm through only a small opening. Some normal ergastoplasm is present within the inclusions but all other constituents are abnormal. Both normal and degenerating mitochondria occur in the cytoplasm but only degenerating ones are found within the inclusions. Both types of inclusions arise in greatly enlarged cells in which an attempt is made to maintain the normal nuclear surface/nuclear volume ratio by the development of the invaginations of the nuclear envelope.

scholarly journals ELECTRON MICROSCOPY OF LYSOSOME-RICH FRACTIONS FROM RAT LIVER

1956 ◽  
Vol 2 (4) ◽  
pp. 179-184 ◽  
Author(s):  
Alex B. Novikoff ◽  
H. Beaufay ◽  
C. de Duve
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Rat Liver ◽  
Electron Micrographs ◽  
Microscope Study ◽  
Electron Microscope Study ◽  
Internal Cavity ◽  
Dense Granules ◽  
Dense Bodies

A preliminary electron microscope study has revealed the presence in lysosome-rich fractions, isolated from rat liver, of hitherto undescribed cytoplasmic particles, called "dense bodies." Approximately 0.37 µ in length, the dense bodies often possess an internal cavity and external membrane. They contain many electron-dense granules 55 to 77 A, or less, in diameter. Such dense bodies are also visible in electron micrographs of parenchymatous cells in liver sections. The correlations between dense bodies and lysosomes are listed, but until pure preparations are available it is not possible to assert that dense bodies and lysosomes are identical.

Demonstration with the electron microscope of in jected peroxidase in rat liver cells

1964 ◽  
Vol 3 (6) ◽  
pp. 561-564 ◽  
Author(s):  
W. Th. Daems ◽  
M. Ploeg ◽  
J. -P. Persijn ◽  
P. Duijn
Keyword(s):  
Electron Microscope ◽  
Rat Liver ◽  
Liver Cells ◽  
Rat Liver Cells
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