Isolation of the respiratory burst oxidase: The role of a flavoprotein component

1988 ◽  
Vol 20 (6) ◽  
pp. 653-677 ◽  
Author(s):  
John F. Parkinson ◽  
Theodore G. Gabig
Keyword(s):  
Respiratory Burst ◽  
Respiratory Burst Oxidase

scholarly journals Role of a respiratory burst oxidase of Lepidium sativum (cress) seedlings in root development and auxin signalling

2012 ◽  
Vol 63 (18) ◽  
pp. 6325-6334 ◽  
Author(s):  
Kerstin Müller ◽  
Ada Linkies ◽  
Gerhard Leubner-Metzger ◽  
Allison R. Kermode
Keyword(s):  
Root Development ◽  
Respiratory Burst ◽  
Lepidium Sativum ◽  
Auxin Signalling ◽  
Respiratory Burst Oxidase

scholarly journals Generating Novel Male Sterile Tomatoes by Editing Respiratory Burst Oxidase Homolog Genes

2022 ◽  
Vol 12 ◽  
Author(s):  
Xiaojuan Dai ◽  
Huanan Han ◽  
Wei Huang ◽  
Lianghui Zhao ◽  
Minglei Song ◽  
...  
Keyword(s):  
Respiratory Burst ◽  
Hybrid Breeding ◽  
Wild Type ◽  
Male Sterile ◽  
Ros Metabolism ◽  
Reproduction Process ◽  
Self Fertilization ◽  
Mutant Lines ◽  
Respiratory Burst Oxidase

Hybrid breeding of tomatoes (Solanum lycopersicum), an important vegetable crop, is an effective way to improve yield and enhance disease and stress resistance. However, the efficiency of tomato hybridization is hindered by self-fertilization, which can be overcome using male sterile lines. It has been reported that reactive oxygen species (ROS) act as a key regulator for anther development, mediated by RBOH (Respiratory Burst Oxidase Homolog) genes. Here, two tomato anther-expressed genes, LeRBOH (Solyc01g099620) and LeRBOHE (Solyc07g042460), were selected to cultivate novel tomato male sterile strains. By using a CRISPR/Cas9 system with a two-sgRNA module, the lerboh, lerbohe, and lerboh lerbohe mutant lines were generated, among which the lerbohe and lerboh lerbohe mutants displayed complete male sterility but could accept wild-type pollens and produce fruits normally. Further analysis uncovered significantly decreased ROS levels and abnormal programmed cell death in lerboh lerbohe anthers, indicating a key role of ROS metabolism in tomato pollen development. Taken together, our work demonstrates a successful application of gene editing via CRISPR/Cas9 in generating male sterile tomatoes and afforded helpful information for understanding how RBOH genes regulating tomato reproduction process.

Role of plant respiratory burst oxidase homologs in stress responses

2018 ◽  
Vol 52 (8) ◽  
pp. 826-839 ◽  
Author(s):  
Wei Wang ◽  
Dongdong Chen ◽  
Xiaopei Zhang ◽  
Dan Liu ◽  
Yingying Cheng ◽  
...  
Keyword(s):  
Stress Responses ◽  
Respiratory Burst ◽  
Respiratory Burst Oxidase
Sign in / Sign up

Export Citation Format

Share Document