Concentration of nucleic acids and their nucleotide composition in various areas of the dog heart when extracardiac parasympathetic influences are excluded

The composition of nucleic acid preparations isolated from seedlings and leaves of plants grown from vernalized and nonvernalized winter wheat grain was chromatographically investigated on a methylated albumin kieselguhr column (MAK column). The intensity of synthesis of the particular nucleic acid fractions was compared by the <sup>32</sup>P-incorporation technique and the nucleotide composition of the newly synthesized nucleic acid fractions was determined. It was observed that the rate of <sup>32</sup>P incorporation into the sRNA, rRNA+mRNA and TB-RNA fractions of the leaves of vernalized plants was about two times higher as compared with that in the nonvernalized control.

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Electron Microscopy of Nucleic Acids

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051529 ◽

1974 ◽

Vol 32 ◽

pp. 302-303

Author(s):

Norman Davidson

Keyword(s):

Electron Microscopy ◽

Nucleic Acids ◽

Basic Protein ◽

Molecular Biologist ◽

Topological Properties ◽

Protein Film ◽

Polynucleotide Chain

The basic protein film technique for mounting nucleic acids for electron microscopy has proven to be a general and powerful tool for the working molecular biologist in characterizing different nucleic acids. It i s possible to measure molecular lengths of duplex and single-stranded DNAs and RNAs. In particular, it is thus possible to as certain whether or not the nucleic acids extracted from a particular source are or are not homogeneous in length. The topological properties of the polynucleotide chain (linear or circular, relaxed or supercoiled circles, interlocked circles, etc. ) can also be as certained.

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mRNA localization by Electron microscopic in situ hybridization

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100086453 ◽

1991 ◽

Vol 49 ◽

pp. 430-431

Author(s):

J. A. Pollock ◽

M. Martone ◽

T. Deerinck ◽

M. H. Ellisman

Keyword(s):

Electron Microscopy ◽

High Resolution ◽

Nucleic Acids ◽

Recombinant Dna ◽

Light And Electron Microscopy ◽

Electron Microscopic ◽

High Voltage Electron Microscopy ◽

Functional Sites ◽

Voltage Electron

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.

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