Electrical activity in the dorsal spinal cord horns and the somatosensory cortex of rats with and without a pain syndrome following sciatic nerve transection

1993 ◽  
Vol 115 (5) ◽  
pp. 495-497
Author(s):  
G. N. Kryzhanovskii ◽  
V. K. Reshetnyak ◽  
M. L. Kukushkin ◽  
V. S. Smirnova ◽  
V. A. Zinkevich
Keyword(s):  
Spinal Cord ◽  
Sciatic Nerve ◽  
Electrical Activity ◽  
Somatosensory Cortex ◽  
Pain Syndrome ◽  
Nerve Transection ◽  
Dorsal Spinal Cord ◽  
Sciatic Nerve Transection ◽  
Dorsal Spinal

Microglial polarization dynamics in dorsal spinal cord in the early stages following chronic sciatic nerve damage

2016 ◽  
Vol 617 ◽  
pp. 6-13 ◽  
Author(s):  
Fangting Xu ◽  
Juan Huang ◽  
Zhenghua He ◽  
Jia Chen ◽  
Xiaoting Tang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Sciatic Nerve ◽  
Nerve Damage ◽  
Dorsal Spinal Cord ◽  
Microglial Polarization ◽  
Early Stages ◽  
Dorsal Spinal

CB2/miR-124 signaling down-regulate the expression of purinergic P2X4 and P2X7 receptor in dorsal spinal cord of CCI rats

2020 ◽  
Author(s):  
Rui Xu ◽  
Fan Yang ◽  
Lijuan Li ◽  
Xiaohong Liu ◽  
Xiaolu Lei ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Intrathecal Injection ◽  
Receptor Activation ◽  
Cb2 Receptor ◽  
Dorsal Spinal Cord ◽  
P38mapk Pathway ◽  
Dorsal Spinal

Abstract Background: The importance of P2X purinoceptors, CB2 receptor and microRNA-124(miR-124) in spinal cord microglia to the development of neuropathic pain was demonstrated in numerous previous studies. The upregulation of P2X4 and P2X7 receptors in spinal dorsal horn microglia is involved in the development of pain behavior caused by peripheral nerve injury. However, it is not clear whether the expression of P2X4 and P2X7 receptors at dorsal spinal cord will be influenced by CB2 receptor or miR-124 in rats after chronic sciatic nerve injury.Methods: Chronic constriction injury (CCI) of the sciatic nerve was performed in rats to induce neuropathic pain. Tests of the mechanical withdrawal threshold (MWT) were carried out to assess the response of the paw to mechanical stimulus. The expression of miR-124, P2X4, P2X7 and CB2 receptor were detected with RT-PCR. The protein expression of P2X4, P2X7 and CB2 receptor, RhoA, ROCK1, ROCK2, p-p38MAPK and p-NF-kappaBp65 was detected with Western blotting analysis. Results: Intrathecal administration of CB2 receptor agonist AM1241 significantly attenuated CCI-induced mechanical allodynia and significantly inhibited the increased expression of P2X4 and P2X7 receptors at the mRNA and protein levels, which imply that P2X4 and P2X7 receptors expression are down-regulated by AM1241 in CCI rats. Western blot analysis showed that AM1241 suppressed the elevated expression of RhoA, ROCK1, ROCK2, p-p38MAPK and NF-κBp65 in the dorsal spinal cord induced by CCI. After administration with Y-27632 (ROCK inhibitor), SB203580 (P38MAPK inhibitor) or PDTC (NF-κB inhibitor), the levels of P2X4 and P2X7 receptors expression in the dorsal spinal cord were lower than those in CCI rats, which imply that the ROCK/P38MAPK pathway and NF-κB activation may contribute to the increased expression of P2X4 and P2X7 receptor. On the other hand, in CCI rats, AM1241 treatment evoked the increased expression of CB2 receptor and miRNA-124, which can be inhibited by intrathecal injection of CB2 receptor antagonist AM630, which indicate that the increased expression of miRNA-124 may be medicated by CB2 receptor activation. In addition, the increased expression of P2X4 and P2X7 receptors in the dorsal spinal cord of CCI rats were inhibited by miRNA-124 agomir. Furthermore, intrathecal injection of miRNA-124 agomir could efficiently inhibit the ROCK/P38MAPK pathway and NF-κB activation in CCI rats. Moreover, AM1241 treatment significantly inhibited the expression of P2X4 and P2X7 receptors, and this suppression is enhanced by pretreatment with miRNA-124 agomir. On the contrast, the inhibitory effect of AM1241 on the expression of P2X4 and P2X7 receptor can be reversed by pretreatment with miRNA-124 antagomir.Conclusions: In CCI rats, intrathecal injection of AM1241 could efficiently induce the increased expression of miRNA-124, while inhibiting the ROCK/P38MAPK pathway and NF-κB activation in dorsal spinal cord. CB2 receptor/miRNA-124 signaling induced the decreased P2X4 and P2X7 receptors expression via inhibit the ROCK/P38MAPK pathway and NF-κB activation.

Neonatal sciatic nerve transection induces TUNEL labeling of neurons in the rat spinal cord and DRG

Neuroreport ◽  
1997 ◽  
Vol 8 (13) ◽  
pp. 2837-2840 ◽  
Author(s):  
A L. R. Oliveira ◽  
M Risling ◽  
M Deckner ◽  
T Lindholm ◽  
F Langone ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Sciatic Nerve ◽  
Nerve Transection ◽  
Rat Spinal Cord ◽  
Sciatic Nerve Transection

scholarly journals Effect of Sciatic Nerve Transection on acetylcholinesterase activity in spinal cord and skeletal muscles of the bullfrog Lithobates catesbeianus

2017 ◽  
Vol 78 (2) ◽  
pp. 217-223
Author(s):  
A. Kroth ◽  
V. Mackedanz ◽  
C. Matté ◽  
A. T. S. Wyse ◽  
M. F. M. Ribeiro ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Skeletal Muscles ◽  
Ache Activity ◽  
Clinical Symptoms ◽  
Phantom Limb ◽  
Nerve Transection ◽  
Sciatic Nerve Transection ◽  
Lithobates Catesbeianus

Abstract Sciatic nerve transection (SNT), a model for studying neuropathic pain, mimics the clinical symptoms of “phantom limb”, a pain condition that arises in humans after amputation or transverse spinal lesions. In some vertebrate tissues, this condition decreases acetylcholinesterase (AChE) activity, the enzyme responsible for fast hydrolysis of released acetylcholine in cholinergic synapses. In spinal cord of frog Rana pipiens, this enzyme’s activity was not significantly changed in the first days following ventral root transection, another model for studying neuropathic pain. An answerable question is whether SNT decreases AChE activity in spinal cord of frog Lithobates catesbeianus, a species that has been used as a model for studying SNT-induced neuropathic pain. Since each animal model has been created with a specific methodology, and the findings tend to vary widely with slight changes in the method used to induce pain, our study assessed AChE activity 3 and 10 days after complete SNT in lumbosacral spinal cord of adult male bullfrog Lithobates catesbeianus. Because there are time scale differences of motor endplate maturation in rat skeletal muscles, our study also measured the AChE activity in bullfrog tibial posticus (a postural muscle) and gastrocnemius (a typical skeletal muscle that is frequently used to study the motor system) muscles. AChE activity did not show significant changes 3 and 10 days following SNT in spinal cord. Also, no significant change occurred in AChE activity in tibial posticus and gastrocnemius muscles at day 3. However, a significant decrease was found at day 10, with reductions of 18% and 20% in tibial posticus and gastrocnemius, respectively. At present we cannot explain this change in AChE activity. While temporally different, the direction of the change was similar to that described for rats. This similarity indicates that bullfrog is a valid model for investigating AChE activity following SNT.

mRNA and protein expression and activities of nitric oxide synthases in the lumbar spinal cord of neonatal rats after sciatic nerve transection and melatonin administration

2006 ◽  
Vol 407 (2) ◽  
pp. 182-187 ◽  
Author(s):  
Fábio Rogério ◽  
Simone Aparecida Teixeira ◽  
Hamilton Jordão Júnior ◽  
Carla Cristina Judice Maria ◽  
André Schwambach Vieira ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Spinal Cord ◽  
Sciatic Nerve ◽  
Lumbar Spinal Cord ◽  
Nerve Transection ◽  
Neonatal Rats ◽  
Sciatic Nerve Transection ◽  
Melatonin Administration ◽  
Mrna And Protein Expression ◽  
Lumbar Spinal

Differences in the neuronal activity of dorsal horn neurons between rats developing and not developing a pain syndrome after sciatic nerve transection

1995 ◽  
Vol 119 (5) ◽  
pp. 452-455
Author(s):  
G. N. Kryzhanovskii ◽  
V. K. Reshetnyak ◽  
M. L. Kukushkin ◽  
V. A. Zinkevich ◽  
V. S. Smirnova
Keyword(s):  
Sciatic Nerve ◽  
Dorsal Horn ◽  
Neuronal Activity ◽  
Pain Syndrome ◽  
Nerve Transection ◽  
Sciatic Nerve Transection ◽  
Dorsal Horn Neurons
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