Immunological specificity of rapid rejection of a skin graft through antigenic overlap in the H-2 system: Role of immune lymphocytes and antibodies

Receptors of effector T lymphocytes of congeneic strains of mice do not recognize public H-2 specificities and react to private H-2 specificities only. This has been established with the use of three tests: direct cytotoxicity assay of immune lymphocytes upon target cells, specific absorption of the lymphocytes on the target cells, and rejection of skin grafts at an accelerated fashion. Immunization with two private H-2 specificities in the system C57BL/10ScSn leads to B10.D2 induces formation of two corresponding populations of effector lymphocytes in unequal proportion: a greater part of them is directed against the private specificity H-2.33 (Kb), while the smaller part is towards H-2.2 (Db) private specificity. These two populations of effector lymphocytes do not overlap, as demonstrated by experiments on their cross-absorption on B10.D2 (R107), B10.D2 (R101), B10.A(2R), and B10.A(5R) target cells, as well as on mixtures of R107 and R101 targets. Following removal of lymphocytes reacting with one of the private H-2 specificities, lymphocytes specific to the other specificity are fully maintained. A mixture of target cells, each bearing one of the two immunizing private specificities, absorbs 100% of the immune lymphocytes and is totally destroyed by them. It is suggested that H-2 antigens are natural complexes of hapten-carrier type, in which the role of hapten is played by public H-2 specifities and that of the carrier determinant by either private H-2 specificities or structures closely linked to them. Various models of steric arrangement of MHC determinants recognized by receptors of effector T lymphocytes are discussed.

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Role of Free Radicals in Necrosis of Skin Graft Compromised With Hematoma

Annals of Plastic Surgery ◽

10.1097/00000637-200206000-00017 ◽

2002 ◽

Vol 48 (6) ◽

pp. 665-669 ◽

Cited By ~ 6

Author(s):

Michael F. Angel ◽

Feng Zhang ◽

Barbara Rogers ◽

Jeffrey Caplan ◽

Wanda Dorsett???Martin ◽

...

Keyword(s):

Free Radicals ◽

Skin Graft

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Corrigendum to “Role of serum amyloid P in skin graft survival and wound healing in burned patients receiving skin grafts” [Clinica Chimica Acta 412 (2011) 227-229]

Clinica Chimica Acta ◽

10.1016/j.cca.2011.01.001 ◽

2011 ◽

Vol 412 (7-8) ◽

pp. 670

Author(s):

Yi-ming Zhang ◽

Ya-dong Fang ◽

Yi-cheng Wang ◽

Shao-liang Wang ◽

Ze-yuan Lei ◽

...

Keyword(s):

Wound Healing ◽

Graft Survival ◽

Skin Graft ◽

Serum Amyloid ◽

Skin Grafts ◽

Serum Amyloid P ◽

Amyloid P

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THE ROLE OF T4+ AND LY-2+ CELLS IN SKIN GRAFT REJECTION IN THE MOUSE

Transplantation Journal ◽

10.1097/00007890-198708000-00019 ◽

1987 ◽

Vol 44 (2) ◽

pp. 273-279 ◽

Cited By ~ 18

Author(s):

Jenni Wheelahan ◽

Ian F.C. Mckenzie

Keyword(s):

Skin Graft ◽

Graft Rejection

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THE ROLE OF HUMORAL ANTIBODIES IN REJECTION OF SKIN HOMOGRAFTS IN RABBITS

Journal of Experimental Medicine ◽

10.1084/jem.114.4.509 ◽

1961 ◽

Vol 114 (4) ◽

pp. 509-520 ◽

Cited By ~ 16

Author(s):

Roberto R. Kretschmer ◽

Ruy Peréz-Tamayo

Keyword(s):

Immune Responses ◽

Skin Graft ◽

Local Administration ◽

Cellular Immune Responses ◽

Humoral Antibodies ◽

Skin Homograft ◽

Cellular Immune ◽

Control Skin

Gross and microscopic observations of skin homograft rejection carried out in cortisone-conditioned and non-conditioned rabbits seem to indicate that humoral antibodies play an important role in the phenomenon. Thus, local administration of isoimmune serum to animals bearing skin homografts resulted in a significantly earlier rejection of that particular test graft without modifying the course of a neighboring control-skin graft. This result appears to support the idea that homograft rejection is not only due to cellular antibodies but to a combination of both humoral and cellular immune responses, which should not be regarded as completely unrelated.

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ROLE OF REACTIVE NITROGEN INTERMEDIATES IN THE REGULATION OF ALLOGENEIC SKIN GRAFT SURVIVAL IN MICE AFTER PORTAL VEIN PRETRANSPLANT TRANSFUSION

Transplantation Journal ◽

10.1097/00007890-199510150-00016 ◽

1995 ◽

Vol 60 (7) ◽

pp. 707-713 ◽

Cited By ~ 8

Author(s):

R M Gorczynski ◽

B Rossi-Bergman ◽

B Sullivan ◽

Z Chen

Keyword(s):

Portal Vein ◽

Graft Survival ◽

Skin Graft ◽

Reactive Nitrogen ◽

Reactive Nitrogen Intermediates

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The hemostatic and wound healing effects of autologous fibrin adhesive in experimental animals

Tạp chí Sinh lý học Việt Nam ◽

10.54928/vjp.v25i2.10 ◽

2021 ◽

Vol 25 (2) ◽

pp. 45-54

Author(s):

Thu Soan Hoang ◽

Tien Thang Vu ◽

Thi Kim Lien Vu ◽

Thi Kieu Van Bui ◽

Hoang Phuong Thuy Vu ◽

...

Keyword(s):

Wound Healing ◽

Fibrin Glue ◽

Skin Graft ◽

Skin Grafting ◽

Fibrin Adhesive ◽

Experimental Animals ◽

Sewing Thread ◽

Wound Healing Effect ◽

Surrounding Skin

Objectives: Evaluate the hemostasis and skin graft wound healing effect of autologous fibrin adhesive in experimental animals. Methods: experimental method, conducted on 6 rabbits to determine the role of fibrin glue in hemostasis, ability to heal skin graft in experimental rabbits through assessment of graft status: size, swelling, color, boundary between the puzzle piece and the surrounding skin. Results: 1/ Effective hemostasis: fibrin glue transplant: hemostasis time is 129.33±42.01 seconds, transplant time is 128.33±41.87 seconds; suture stitching: hemostasis time is 475.17±59.97 seconds, stitching time is 350.67±52.23 seconds. At times after 1 day, 3 days, 7 days, 10 days, 14 days, 20 days and 30 days there is an earlier phenomenon of wound healing, the flattened and scarred patch is more beautiful than the skin graft with sewing thread. Conclusions: Using autologous fibrin glue in skin grafting has the ability to stop bleeding, heal wounds and achieve better aesthetics than skin grafting with sutures.

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SYNERGISTIC INTERACTION OF MACROPHAGES AND LYMPHOCYTES IN ANTIGEN-INDUCED TRANSFORMATION OF LYMPHOCYTES

Journal of Experimental Medicine ◽

10.1084/jem.132.1.44 ◽

1970 ◽

Vol 132 (1) ◽

pp. 44-65 ◽

Cited By ~ 123

Author(s):

Robert C. Seeger ◽

Joost J. Oppenheim

Keyword(s):

Tetanus Toxoid ◽

Incubation Time ◽

Guinea Pigs ◽

Thymidine Incorporation ◽

Tritiated Thymidine ◽

Lymphocyte Transformation ◽

Purified Protein Derivative ◽

Immune Lymphocytes ◽

Antigen Interaction

The role of macrophages and lymphocytes in antigen-induced transformation of lymphocytes has been investigated. Lymphocytes and macrophages were obtained from inbred strain 13 guinea pigs which were either unimmunized or immunized with complete Freund's adjuvant (CFA) or tetanus toxoid in CFA. The transformation response to PPD or tetanus toxoid was assayed by tritiated thymidine incorporation. Addition of macrophages to immune lymphocytes significantly increased their response to purified protein derivative (PPD) or tetanus toxoid. This was observed if the macrophages were (a) "immune" or "nonimmune", (b) unirradiated or irradiated (3000 R), (c) 99% pure, and (d) peritoneal or alveolar in origin. Neither immune nor nonimmune macrophages were able to induce nonimmune lymphocytes to respond to PPD or tetanus toxoid. When macrophages were incubated with PPD or tetanus toxoid and then washed, they stimulated immune lymphocytes to transform. An incubation time of ½ hr was adequate, however, 2–4: hr was optimal. These studies indicate (a) that antigen-induced transformation of lymphocytes is greatly enhanced by macrophages; (b) that macrophage-antigen interaction can antecede lymphocyte-antigen interaction and results in macrophages which are able to stimulate lymphocyte transformation; and (c) that the immunological memory requisite to elicit specific transformation responses is a property of the lymphocyte and not the macrophage.

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