Effects of GYDENPHY caspules on streptozotocin-induced type 1 diabetic in Swiss mouse model

Objective: Evaluation the hypoglycemic effect of Gydenphy capsules on Streptozotocin-induced type 1 diabetic in Swiss mouse model. Methods: The type 1 diabetic model was established by intraperitoneal injections of Streptozocin 150mg/kg in Swiss mouse. Then, the Gydenphy were orally administered daily at a dose of 576 mg/kg/day or 1152 mg/kg/day in 10 days. Blood glucose concentration in the Gydenphy oral groups with that of water control group and the intraperitoneal insulin injection group was compared. Results: Blood glucose concentration in the groups using Gydenphy (dose576 mg/kg/24h and dose 1152 mg/kg/24h) significal decreased compared to the distilled water group at (p <0.05 at the time of 4 hours, 8 hours; p <0.01 at the time of 3, 10 days). The hypoglycemic effect of Gydenphy at 576mg/kg/day and 1152 mg/kg/day at 4 hours, 8 hours and 3 days were inferior to insulin 0.1 UI/kg/day for glycemic control. However, the hypoglycemic effect ofGydenphy were equivalent to insulin after 10 consecutive days on treatment. Conclusion: Gydenphy capsules have hypoglycemic effects onStreptozotocin-induced type 1 diabetes in Swiss mouse model.

Evaluating the affect of dimethyl sulfoxide on the viablity of H9C2 cardiomyocytes

Objective: The study was conducted to evaluate the affect of Dimethyl Sulfoxide (DMSO) concentration on the viability of H9C2 cells under the different cultural conditions. Methods: H9C2 cells were cultured under normal conditions and subjected to hypoxia/reoxygenation model. DMSOat the doses of 0,001÷10% (v/v) was added to the cultural medium during normal culture period and reoxygenation period. Cellular viability of the experimental groups was assessed by using CCK-8 kit. Results: The results indicated that the viability of H9C2 cardiomyocytes was stable in the different culture media supplied with DMSO at the doses of0,001÷0,5% (v/v). Meanwhile, supplementation of DMSO at the doses of1% and 2% significantly decreased the survival rate of H9C2 cells (v/v,p<0,05). Conclusion: The affect of DMSO on H9C2 cardiomyocytes is dose-dependant maner.

Effects of NP on some hematological and immunological indices in sarcoma TG180-bearing mice

Objective: Evaluate the effects of saponin-rich extracts from Panax notoginseng roots with steaming - NP (H) and without steaming - NP (O) on some hematological and immunological indices in sarcoma TG180-bearing mice. Methods: The mice were injected with Sarcoma TG180 cells in the thigh to cause tumors, then were oral administrated NP(O), NP(H), Letinan or normal saline from the 6th day to 22nd day since developed tumors. On the 22nd day, blood was taken for IL-2, TNF-α and hematological indicators, revealing and determining the relative weight of spleen and thymus. Results: NP(H) and NP(O) at both level of the doses of 300 mg/kg/day and 900 mg/kg/day increased red blood cell count, hemoglobin, hematocrit, adjusted white blood cell and platelet counts return to physiological level, increased blood IL-2 and TNF-α, increased relative weight of spleen and thymus. Conclusion: The saponin-rich extracts from Panax notoginseng roots had the effects of improving hematological index and enhancing immunity on sarcoma 180-bearing mice, in which the extract from steaming Panax notoginseng roots has a better effect compared with no-steaming Panax notoginseng roots ones.

Influence of media and cellular origin on growth factors secreted by mesenchymal stem cells

Objective: Investigating influences of the culture media and cellular origin on the expression of several growth factors carried by exosomes secreted from umbilical cord-derived mesenchymal stem cells (UCMSC) and bone marrow-derived mesenchymal stem cells (BMMSC). Methods: 03 UCMSC samples was cultured in StemMACs and PowerStem, 03 BMMSC samples was cultured in StemMAC sand ACF Plus. At the passage 2, conditioned media were collected for exosome isolation. Exosomes were then investigated for the expression of growth factors, including: vascular endothelial growth factor A (VEGF-A), fibroblast growth factor 2(FGF-2), hepatocellular growth factor (HGF) and platelet-derived growth factor BB(PDGF-BB). Results: Both UCMSC and BMMSC at secreted exosomes carrying growth factors with different amount. UCMSC cultured in PowerStem could secret exosomes with the largest amount of growth factors. Besides, UCMSC and BMMSC cultured in StemMACs secreted exosomes carrying fewer amounts of growth factors. Secretion of growth factors through exosomes depends on the cell origin where both cell types were cultured in StemMACs, only UCMSC secretedFGF-2 and PDGF-BB but BMMSC did not. Conclusion: Both UCMSC and BMMSC cultured in commercial serum- and xeno-free media could secret exosomes into cell culture media. These exosomes carried growth factors, including VEGF-A, FGF-2, HGF and PDGF-BB, with different expression levels.

Bleeding after surgery and application bleeding assessment tool to surgical patients in Hanoi Medicine University Hospital

Objectives: Describe bleeding characteristics and evaluate the correlation between surgical-related bleeding and bleeding risk according by ISTH – BATs. Methods: Research was conducted on 340 surgical patients at Hanoi Medical University Hospital. Results: The percentage of patients with bleeding during and after surgery is 13.5%. The proportion of patients at risk of bleeding according to BATs is 1.8%. There was a correlation between bleeding risk according to ISTH - BAT with bleeding status during and after surgery with p = 0.004. The positive predictive value of ISTH - BATs is 66.7%, negative predictive value is 87.4%, the sensitivity is 8.7%, the specificity is 99.3%. Conclusions: Surgery has a high risk of abnormal bleeding. Bleeding history has important implications in assessing bleeding risk during and after surgery. The ISTH - BATs is a bleeding history assessment tool that can be used to assess the risk of bleeding before surgery.

The hemostatic and wound healing effects of autologous fibrin adhesive in experimental animals

Objectives: Evaluate the hemostasis and skin graft wound healing effect of autologous fibrin adhesive in experimental animals. Methods: experimental method, conducted on 6 rabbits to determine the role of fibrin glue in hemostasis, ability to heal skin graft in experimental rabbits through assessment of graft status: size, swelling, color, boundary between the puzzle piece and the surrounding skin. Results: 1/ Effective hemostasis: fibrin glue transplant: hemostasis time is 129.33±42.01 seconds, transplant time is 128.33±41.87 seconds; suture stitching: hemostasis time is 475.17±59.97 seconds, stitching time is 350.67±52.23 seconds. At times after 1 day, 3 days, 7 days, 10 days, 14 days, 20 days and 30 days there is an earlier phenomenon of wound healing, the flattened and scarred patch is more beautiful than the skin graft with sewing thread. Conclusions: Using autologous fibrin glue in skin grafting has the ability to stop bleeding, heal wounds and achieve better aesthetics than skin grafting with sutures.

Prevalance and characteristics of sarcopenia in older patients with stable chronic obstructive pulmonary disease

Objectives: To determine the rate and characteristics of sarcopenia in older patients with stable chronic obstructive pulmonary disease. Subjects and methods: a cross-sectional descriptive study on 363 stable chronic obstructive pulmonary disease (COPD) patients aged ≥ 60 years old examined and treated at the National Geriatrics Hospital and 19-8 Hospital. Study subjects were measured appendicular skeletal muscle with dual energy adsorption radiographs and diagnosed with sarcopenia based on the criteria of Asian Working Group for sarcopenia – AWGS. Results: Average age of study subjects was 70.7 ± 8.3 (years), male rate was 60.3%. The prevalence of sarcopenia in the study group was 45.2%. 74.6% of patients had a decrease in total appendicular skeletal muscle according to height (ASMI), 68.3% had a decrease in handgrip strength (HGS) and 42.7% had a decrease in walking speed. The ASMI, handgrip strength and walking speed decreased with age. Conclusion: The study highlighted a high rate of sarcopenia (45.2%) in elderly patients with stable stage COPD. The ASMI, handgrip strength and walking speed decreased with age. Therefore, it is necessary to screening and early diagnosis of sarcopenia in elderly COPD patients.

Study on characteristics of gene mutation G6PD in patient of enzyme G6PD deficiency

G6PD deficiency is the most common genetic enzyme pathology in humans. The disease is caused by a gene mutation G6PD-the majority of cases are asymptomatic when exposed to oxidizing agents (drugs, chemicals, or foods). The disease is temporarily stopped at symptomatic treatment, so early detection is to help improve the quality of life for the patient, to prevent possible complications. The study was conducted to detect mutations in all regions of the gene G6PD. Through the study of 262 boys, 38 girls in 25 provinces in the northern region of Vietnam of 5 ethnic groups showed the results finding 11 deviated mutations that affect the coding region of the G6PD gene is Viangchan (c.871G> A) (24.33%), Kaipping (c.1388G> A) (22.67%), Canton (c.1376G> T) (18.67%), Union (c.1360C> T) (13.33%), Gaohe (cc95A > G) (6.67%), QuingYuang (c.392G> T) (4.33%), Chiniese-5 (c.1024C> T) (4.33%), Orissa (c.131C> G) (1.33%) Chatham (c.1003G> A) (1%) and Taiwan2 (c.1330G> A) (0.33%); 78/297 cases of silent mutation (c.1311C> T) associated with most of the above mutations. Discovered 2 mutant forms Orisa and Taiwan 2 that have never appeared in Vietnam. These mutations are spread evenly among the Kinh, Muong, Tay, Nung and Thai ethnic groups, of which the Kinh mainly carry the Viangchan mutation, the remaining Muong are the Union and the Tay, Nung Thai are most of Kaiping mutation

Energy expenditure during cardiopulmonary exercise testing

Objectives: To determine energy expenditure during cardiopulmonary exercise testing in healthy adults aged 20-60 years old and to determine the correlation between energy expenditure with the duration and the capacity of physical activity. Methods: Thirty-two healthy volunteers aged 20-60 years old (17 males and 15 females) agreed to participate in our study and performed cardiopulmonary exercise testing: resting phase (4 minutes, work rate 0 w), warming-up phase (4 minutes, work rate 0 w, 50-60 rounds per minute), exercise phase (work rate increment 10 watts per minutes, 50-60 rounds per minute) and recovery phase (3 minutes, work rate 0 w, 10-20 rounds per minute). Results and conclusions: Energy expenditure during CPET was 1,03 ± 0,30 MET, 1,97 ± 0,74 MET, 3,48 ± 1,78 MET, 4,57 ± 2,26 in resting, warming-up, exercise, recovery phase, respectively. Maximum energy expenditure during CPET was 4,75 ± 2,17 MET. Energy expenditure is negatively correlated with the duration of physical activity and positively correlated with the capacity of physical activity at 95% confidence interval.

Efficacy of Botulinum toxin A in treatment of primary hemifacial spasm

Objective: Evaluating the effects of toxin botulinum A in treatment of primary hemifacial spasm. Methods: A total of 48 patients with primary hemifacial spasm were treated by injections of botulinum toxin A (Dysport) in Tam Anh Hospital from 6/2020 to 5/ 2021. Methods: Prospective, descriptive study. Results: Forty-eight patients (8 male, 40 female) were included in the study, the ages were from 36 to 79 years with an average age of 57 ± 10,43 years, having mean Jankovic disability rating scale score of 3.25±0.55SD. MRI revealed vascular compression in all patients. The dose Dysport was 80-145UI; 91,7% reported improvement after 2 weeks - 1 month from the injected. Benefits begin in 2-7 days after the treatment and last for an average of 5,50 ± 0,97 months (3-8 months). Side effects such as ectropion (45,83%), ptosis (4,2%), and hematoma (12,5%) are transient and reversible after 1-3 weeks. No severe complication was noted. Conclusions: This finding show that local botulinum toxin treatment provides effective and safe relief of hemifacial spasm.