Inhibition of formation of stromal cell colonies of human bone marrow by a factor formed in vitro by peripheral blood leukocytes

Murine IgM monoclonal antibody STRO-1 identifies a cell surface antigen expressed by stromal elements in human bone marrow (BM). STRO-1 binds to approximately 10% of BM mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage [CFU-GM], erythroid bursts [BFU-E], and mixed colonies [CFU-Mix]). Fibroblast colony-forming cells (CFU-F) are present exclusively in the STRO-1+ population. Dual-color cell sorting using STRO-1 in combination with antibody to glycophorin A yields a population approximately 100-fold enriched in CFU-F in the STRO-1+/glycophorin A+ population. When plated under long-term BM culture (LTBMC) conditions, STRO-1+ cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements. STRO-1+ cells isolated from LTBMC at later times retain the capacity to generate adherent layers with a cellular composition identical to that of the parent cultures. The STRO-1-selected adherent layers are able to support the generation of clonogenic cells and mature hematopoietic cells from a population of CD34+ cells highly enriched in so-called long-term culture-initiating cells. We conclude that antibody STRO-1 binds to BM stromal elements with the capacity to transfer the hematopoietic microenvironment in vitro.

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The effects of thiazolidinediones on human bone marrow stromal cell differentiation in vitro and in thiazolidinedione-treated patients with type 2 diabetes

Translational Research ◽

10.1016/j.trsl.2012.08.006 ◽

2013 ◽

Vol 161 (3) ◽

pp. 145-155 ◽

Cited By ~ 38

Author(s):

George R. Beck ◽

Natasha B. Khazai ◽

Gary F. Bouloux ◽

Corinne E. Camalier ◽

Yiming Lin ◽

...

Keyword(s):

Type 2 Diabetes ◽

Bone Marrow ◽

Cell Differentiation ◽

Stromal Cell ◽

Bone Marrow Stromal Cell ◽

Human Bone ◽

Human Bone Marrow ◽

Marrow Stromal Cell

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Faculty Opinions recommendation of The effects of thiazolidinediones on human bone marrow stromal cell differentiation in vitro and in thiazolidinedione-treated patients with type 2 diabetes.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717962161.793465656 ◽

2012 ◽

Author(s):

Tim Cundy ◽

Andrew Grey

Keyword(s):

Type 2 Diabetes ◽

Bone Marrow ◽

Cell Differentiation ◽

Stromal Cell ◽

Bone Marrow Stromal Cell ◽

Human Bone ◽

Human Bone Marrow ◽

Marrow Stromal Cell

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Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1

Blood ◽

10.1182/blood.v78.1.55.55 ◽

1991 ◽

Vol 78 (1) ◽

pp. 55-62 ◽

Cited By ~ 800

Author(s):

PJ Simmons ◽

B Torok-Storb

Keyword(s):

Monoclonal Antibody ◽

Bone Marrow ◽

Stromal Cell ◽

Mononuclear Cells ◽

Human Bone ◽

Human Bone Marrow ◽

Adherent Cell ◽

Glycophorin A

Abstract Murine IgM monoclonal antibody STRO-1 identifies a cell surface antigen expressed by stromal elements in human bone marrow (BM). STRO-1 binds to approximately 10% of BM mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage [CFU-GM], erythroid bursts [BFU-E], and mixed colonies [CFU-Mix]). Fibroblast colony-forming cells (CFU-F) are present exclusively in the STRO-1+ population. Dual-color cell sorting using STRO-1 in combination with antibody to glycophorin A yields a population approximately 100-fold enriched in CFU-F in the STRO-1+/glycophorin A+ population. When plated under long-term BM culture (LTBMC) conditions, STRO-1+ cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements. STRO-1+ cells isolated from LTBMC at later times retain the capacity to generate adherent layers with a cellular composition identical to that of the parent cultures. The STRO-1-selected adherent layers are able to support the generation of clonogenic cells and mature hematopoietic cells from a population of CD34+ cells highly enriched in so-called long-term culture-initiating cells. We conclude that antibody STRO-1 binds to BM stromal elements with the capacity to transfer the hematopoietic microenvironment in vitro.

Download Full-text