Effect of immune complexes on electrophoretic mobility of sheep's red blood cells

1990 ◽  
Vol 109 (3) ◽  
pp. 365-367
Author(s):  
N. A. Konstantinova ◽  
A. M. Sapozhnikov
Keyword(s):  
Red Blood Cells ◽  
Electrophoretic Mobility ◽  
Immune Complexes ◽  
Blood Cells ◽  
Sheep's Red Blood Cells

scholarly journals Electrophoretic Mobility of Red Blood Cells in Patients with Severe Forms of Intoxication

2007 ◽  
Vol 3 (6) ◽  
pp. 75
Author(s):  
I. Ya. Golovetsky ◽  
V. V. Moroz ◽  
L. S. Biryukova ◽  
G. I. Kozinets ◽  
O. V. Popova
Keyword(s):  
Red Blood Cells ◽  
Electrophoretic Mobility ◽  
Blood Cells

scholarly journals ELECTROPHORETIC MOBILITY AND AGGLUTINABILITY OF RED BLOOD CELLS: A "NEW" POLYMORPHISM IN MICE

1974 ◽  
Vol 139 (2) ◽  
pp. 313-322 ◽  
Author(s):  
Pablo Rubinstein ◽  
Ngukoy Liu ◽  
Edwin W. Streun ◽  
Francine Decary
Keyword(s):  
Red Blood Cells ◽  
Genetic Control ◽  
Electrophoretic Mobility ◽  
Blood Cells ◽  
Quantitative Method ◽  
Coat Color ◽  
Inbred Strains ◽  
Red Cells ◽  
Inbred Strains Of Mice

A quantitative method has been developed to determine agglutinability of mouse red blood cells. Tests with different inbred strains of mice revealed only two phenotypes. The same inbred strains were tested with the cytopherometer to determine the electrophoretic mobility of the corresponding red cells. Again, two phenotypes were uncovered, and faster mobility was found in the red cells that had higher agglutinability. The genetic control of this character is autosomal and codominant, and segregates independently of H-2 and coat color.

Optimization and application of electrophoretic mobility analysis of human red blood cells to study their in vitro stability, interaction with polycations and proteolytic enzymes

2007 ◽  
Vol 84A (2) ◽  
pp. 535-544 ◽  
Author(s):  
Christian Grandfils ◽  
Patricia Foresto ◽  
Bibiana Riquelme ◽  
Juana Valverde ◽  
Danièle Sondag‐Thull
Keyword(s):  
Red Blood Cells ◽  
Electrophoretic Mobility ◽  
Blood Cells ◽  
Proteolytic Enzymes ◽  
Mobility Analysis ◽  
Human Red Blood Cells ◽  
In Vitro Stability

Actions of thiocyanate and N-phenylmaleimide on volume-responsive Na and K transport in dog red cells

1992 ◽  
Vol 262 (2) ◽  
pp. C418-C421 ◽  
Author(s):  
J. C. Parker ◽  
G. C. Colclasure
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Electrophoretic Mobility ◽  
Blood Cells ◽  
Membrane Transporters ◽  
Red Cells ◽  
Transport Systems ◽  
Volume Changes ◽  
Sulfhydryl Reagent ◽  
K Transport

Two sets of observations suggest a linkage between volume-responsive Na and K transport systems in dog red blood cells. 1) The lyotropic anion thiocyanate inhibits shrinkage-induced Na-H exchange and stimulates swelling-induced K-Cl cotransport. 2) The effect of a brief incubation with N-phenylmaleimide (NPM) on Na and K transport depends on the volume of the cells at the time of exposure to the sulfhydryl reagent. Cells shrunken during the NPM incubation and then brought back to normal volume behave as though they were still shrunken, i.e., they show an increased Na flux and a decreased K flux. Cells incubated with NPM in a swollen state retain fluxes characteristic of swollen cells when returned to a normal volume. The electrophoretic mobility of the membrane-associated enzyme glyceraldehyde-3-phosphate dehydrogenase is influenced by the cell volume at the time of NPM exposure. These findings point to the existence of a system in cells that perceives volume changes and coordinates the responses of membrane transporters.

DIAGNOSTIC CAPABILITIES OF THE ELECTROPHORETIC MOBILITY OF RED BLOOD CELLS AND BUCCAL CELLS IN STRESS

2019 ◽  
pp. 106-111
Author(s):  
А.В. Дерюгина ◽  
М.Н. Иващенко ◽  
П.С. Игнатьев ◽  
А.Г. Самоделкин ◽  
М.В. Золотова ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Red Blood Cells ◽  
Electrophoretic Mobility ◽  
Blood Cells ◽  
Information Technologies ◽  
Stress Reaction ◽  
Medical Laboratory ◽  
Sharp Change ◽  
Epinephrine Injection ◽  
Acting Force

Диагностика стресс-реактивности по анализу гормонального состояния организма, является сложной, экономически затратной и трудоемкой процедурой исследования. В связи с этим актуален поиск информационных технологий, которые достаточно легко реализуются в любой медицинской лаборатории и позволяют оценить стресс-реакцию организма. Цель работы - оценка диагностических возможностей электрофоретической подвижности эритроцитов и клеток буккального эпителия в процессе развития стрессовой реакции. Методика. Для моделирования стресса использовали внутрибрюшинное введение крысам раствора адреналина-гидрохлорида (0,1 мг/кг) однократно или ежедневно в течение 3 сут эксперимента соответственно. Контролем служили крысы, получавшие физиологический раствор (в/б) в том же объеме. Буккальный эпителий исследовали на 7-е сут после введения адреналина. Мазки клеток эпителия окрашивали по Романовскому-Гимза, на препаратах подсчитывали количество клеток с микроядрами на 1000 клеток. Электрофоретическую подвижность эритроцитов оценивали методом микроэлектрофореза. Результаты. Наблюдалась качественно однотипная динамика показателя электрофоретической подвижности эритроцитов, количественно зависящая от силы воздействия. При слабых стимулах скорость изменений электрофоретической подвижности эритроцитов незначительна и сочетается с развитием адаптивных процессов, связанных с репарацией генетических повреждений. Увеличение степени стрессового воздействия путем трехкратного введения адреналина приводит к резкому изменению электрофоретической подвижности эритроцитов и росту числа эпителиоцитов с различными патологическими изменениями. Заключение. Показана возможность использования данного метода в качестве маркерной характеристики интенсивности стрессовой реакции с выявлением ее генотоксичности. Diagnostics of stress reaction using an analysis of hormonal state is a difficult, costly, and labor-consuming research. Therefore, searching for information technologies, which could be easily used in any medical laboratory and would allow evaluating the stress reaction is relevant. We studied the electrophoretic mobility of red blood cells (RBC) and buccal epithelial cells of rats exposed to stress induced by a single or repeated intraperitoneal epinephrine injection. This study showed that these methods could be used as the marker characteristics of stress reaction intensity and genotoxicity. Qualitative changes in the electrophoretic mobility of RBC were similar but quantitative characteristics depended on the acting force. Under a weak stimulus, changes in the electrophoretic mobility of RBC were slight and associated with development of adaptive processes related with repair of genetic damage. A further increase in the acting force led to a sharp change in the electrophoretic mobility of RBC and an increase in the number of epithelial cells with pathological alterations.

Correlation between electrophoretic mobility and extent of aggregation of human red blood cells

2006 ◽  
Vol 32 (6) ◽  
pp. 748-749
Author(s):  
I. A. Tikhomirova ◽  
A. V. Murav’ev ◽  
L. A. Mikhailichenko ◽  
E. V. Golubkova
Keyword(s):  
Red Blood Cells ◽  
Electrophoretic Mobility ◽  
Blood Cells ◽  
Human Red Blood Cells
Sign in / Sign up

Export Citation Format

Share Document