Preparative isolation and short-term organ culture of imaginal discs ofDrosophila melanogaster

1979 ◽  
Vol 5 (2) ◽  
pp. 1055-1062 ◽  
Author(s):  
Odessa Eugene ◽  
Mary Alice Yund ◽  
James W. Fristrom
Keyword(s):  
Organ Culture ◽  
Imaginal Discs ◽  
Short Term ◽  
Preparative Isolation

IN VITRO METHODS FOR THE STUDY OF THE ADENOHYPOPHYSIAL FUNCTIONS TO SECRETE GONADOTROPHIN

1971 ◽  
Vol 68 (1_Suppl) ◽  
pp. S27-S40 ◽  
Author(s):  
T. Kobayashi ◽  
T. Kigawa ◽  
M. Mizuno ◽  
T. Watanabe
Keyword(s):  
Cell Culture ◽  
Organ Culture ◽  
Cultured Cells ◽  
Cell Sheet ◽  
Short Term ◽  
Hypothalamic Extract ◽  
Numerous Cell ◽  
Single Cell Culture ◽  
Almost All

ABSTRACT There are several in vitro methods to analyse the function of the adenohypophysis or the mechanisms of its regulation. The present paper deals with single cell culture, organ culture and short term incubation techniques by which the morphology and gonadotrophin-secreting function of the adenohypophysis were studied. In trypsin-dispersed cell culture, the adenohypophysial cells showed extensive propagation to form numerous cell colonies and finally develop into a confluent monolayer cell sheet covering completely the surface of culture vessels. Almost all of the cultured cells, however, became chromophobic, at least at the end of the first week of cultivation, when gonadotrophin was detectable neither in the culture medium nor in the cells themselves. After the addition of the hypothalamic extract, gonadotrophin became detectable again, and basophilic or PAS-positive granules also reappeared within the cells, suggesting that the gonadotrophs were stimulated by the extract to produce gonadotrophin. In organ culture and short term incubation, the incorporation of [3H] leucine into the adenohypophysial cells in relation to the addition of hypothalamic extract was examined. It was obvious that the ability to incorporate [3H] leucine into the gonadotrophs in vitro was highly dependent upon the presence of the hypothalamic extract.

scholarly journals Development of a Short-Term Canine Full-Thickness Skin Organ Culture Method under Serum-Free Conditions

2016 ◽  
Vol 11 (2) ◽  
pp. 61-69 ◽  
Author(s):  
Francesca Abramo ◽  
Andrea Pirone ◽  
Carla Lenzi ◽  
Maria Federica della Valle ◽  
Silvia Vidali ◽  
...  
Keyword(s):  
Organ Culture ◽  
Culture Method ◽  
Full Thickness ◽  
Short Term ◽  
Serum Free ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Serum Free Conditions ◽  
Skin Organ Culture

scholarly journals Short-term tissue permeability actions of dextran sulfate sodium studied in a colon organ culture system

2020 ◽  
Vol 8 (2) ◽  
pp. 1728165 ◽  
Author(s):  
Elisabeth M. Danielsen ◽  
Alba De Haro Hernando ◽  
Mohammad Yassin ◽  
Karina Rasmussen ◽  
Jørgen Olsen ◽  
...  
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Dextran Sulfate ◽  
Dextran Sulfate Sodium ◽  
Short Term ◽  
Organ Culture System ◽  
Tissue Permeability

Formation of DNA adducts in human skin maintained in short-term organ culture and treated with coal-tar, creosote or bitumen

1988 ◽  
Vol 42 (4) ◽  
pp. 622-626 ◽  
Author(s):  
Bernadette Schoket ◽  
Alan Hewer ◽  
Philip L. Grover ◽  
David H. Phillips
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Dna Adducts ◽  
Coal Tar ◽  
Short Term

Human colorectal tumours in short-term organ culture A stathmokinetic study

1982 ◽  
Vol 15 (5) ◽  
pp. 555-564 ◽  
Author(s):  
C. J. Pritchett ◽  
P. V. Senior ◽  
J. P. Sunter ◽  
A. J. Watson ◽  
D. R. Appleton ◽  
...  
Keyword(s):  
Organ Culture ◽  
Short Term ◽  
Colorectal Tumours

CIRCADIAN VARIATIONS IN ADRENAL SECRETION OF LEMMINGS, VOLES AND MICE

1970 ◽  
Vol 65 (4) ◽  
pp. 645-649 ◽  
Author(s):  
Richard V. Andrews
Keyword(s):  
Locomotor Activity ◽  
Organ Culture ◽  
Circadian Variation ◽  
The Arctic ◽  
Summer Solstice ◽  
Circadian Variations ◽  
Short Term ◽  
Culture Methods ◽  
Adrenal Secretion

ABSTRACT Daily variations in adrenal secretion by glands from arctic rodents were measured in vitro. Serial-sacrifice, short-term incubation studies yield similar results to those data obtained through organ-culture methods. Adrenal secretory rates display some desynchronization from locomotor activity during the summer solstice, although circadian variation persists during all seasons of the arctic year.

Production of collagenase and inhibitor (TIMP) by intracranial tumors and dura in vitro

1983 ◽  
Vol 59 (3) ◽  
pp. 461-466 ◽  
Author(s):  
Ahmed N. Halaka ◽  
Rowena A.D. Bunning ◽  
Colin C. Bird ◽  
Myles Gibson ◽  
John J. Reynolds
Keyword(s):  
Organ Culture ◽  
Tumor Invasion ◽  
Culture Media ◽  
Intracranial Tumors ◽  
Short Term ◽  
Content Type ◽  
Collagenase Inhibitor ◽  
Tissue Degradation ◽  
Fine Print

✓ The production of collagenase and collagenase inhibitor (TIMP) by various intracranial tumors (25 meningiomas, eight gliomas, seven metastases, four pituitary adenomas, and five others) was studied in short-term organ culture. While meningiomas produced negligible amounts of collagenase, two metastatic carcinomas of bronchial and breast origin produced significant amounts of the enzyme. Cultures of dura from an invasive meningioma and of bone invaded by a meningioma also produced collagenase. In varying amounts, TIMP was detected in culture media from most of the tumors studied; invasive tumors tended to produce less TIMP than noninvasive tumors. The results are discussed in relation to current views on tissue degradation and mechanisms of tumor invasion.

scholarly journals Gram stain and addition of amphotericin B to improve the microbial safety of human donor corneas

2021 ◽  
Author(s):  
Davide Camposampiero ◽  
Adriano Fasolo ◽  
Giuseppe Saccon ◽  
Pietro M. Donisi ◽  
Elisa Zanetti ◽  
...  
Keyword(s):  
At Risk ◽  
Organ Culture ◽  
Amphotericin B ◽  
Statistical Significance ◽  
Positive Culture ◽  
Gram Stain ◽  
Microbial Safety ◽  
Short Term ◽  
Human Donor ◽  
Not At Risk

AbstractTo determine the effectiveness of two methods to improve the microbial safety of human corneas preserved in organ culture. We compared the number of positive preservation solutions of corneas in organ culture in which the initial short-term hypothermic corneal maintenance solution was supplemented with amphotericin B 2.5 µg/mL and the historical data of microbial test results (2015–2019). In addition, we appraised the efficacy of Gram stain to detect bacterial or fungal contamination in the organ culture solutions of corneas from at-risk donors compared to the culture tests of corneas from not-at-risk donors. Statistical analysis was performed using STATA and statistical significance set at p < 0.05. The number of positive culture tests after preservation was 15 (0.5%) in 2020 compared to a mean of 37 (1.2%) in the period 2015–2019 (p < 0.01), with 10 (1.0%) positive samples in the cohort of 998 corneas from at-risk donors and 5 (0.2%) in the 2046 corneas from not-at-risk donors (p < 0.01). All corneas from at-risk donors tested positive at Gram stain and the results were available 1–3 days before those of the conventional culture tests. Amphotericin B supplementation in the short-term maintenance solution markedly reduced the number of positive microbial tests after organ culture and the early detection of contaminants, including slow-growing microorganisms, by Gram stain before the standard culture results. This meant fewer corneas being discarded and a greater likelihood of preventing post-graft infections.

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