Iron limitation and its effect on membrane proteins and siderophore transport inNeurospora crassa

1989 ◽  
Vol 2 (2) ◽  
pp. 108-113 ◽  
Author(s):  
Hans-Georg Huschka ◽  
G�nther Winkelmann
Keyword(s):  
Membrane Proteins ◽  
Iron Limitation ◽  
Siderophore Transport ◽  
Inneurospora Crassa

scholarly journals The effects of iron-limited growth on the reduced nicotinamide–adenine dinucleotide dehydrogenase activity and the membrane proteins of Candida utilis mitochondria

1973 ◽  
Vol 136 (4) ◽  
pp. 1029-1037 ◽  
Author(s):  
Roger A. Clegg ◽  
Jean E. Skyrme
Keyword(s):  
Membrane Proteins ◽  
Mitochondrial Membrane ◽  
Nadh Dehydrogenase ◽  
Candida Utilis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Iron Limitation ◽  
Nicotinamide Adenine Dinucleotide Dehydrogenase ◽  
Reduced Nicotinamide Adenine Dinucleotide ◽  
Haem Iron

1. The mitochondrial NADH dehydrogenase (EC 1.6.99.3) of Candida utilis exhibited altered properties when the organism was grown under iron-limited conditions. No suitable acceptor was found for assay of this enzyme from iron-limited cells. 2. Mitochondrial membrane proteins from C. utilis were analysed by polyacrylamide-gel electrophoresis. Compared with glycerol-limited cells, iron limitation resulted in the loss of at least two polypeptides from the mitochondrial membrane. 3. Neither of the polypeptides affected by iron limitation was part of a cytochrome, although one of them was part of the mitochondrial NADH dehydrogenase. 4. Non-haem iron of mitochondrial membranes was released in the presence of sodium dodecyl sulphate, and electrophoresis in solutions of this detergent cannot be used directly to identify iron–sulphur proteins. Non-ionic detergents do not release non-haem iron but nor do they provide a satisfactory system for electrophoretic separation.

scholarly journals Siderophore production and outer membrane proteins of selectedVibrio vulnificusstrains under conditions of iron limitation

1986 ◽  
Vol 35 (2-3) ◽  
pp. 255-260 ◽  
Author(s):  
Anita C. Wright ◽  
Linda M. Simpson ◽  
Kathleen Richardson ◽  
David R. Maneval ◽  
James D. Oliver ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Outer Membrane Proteins ◽  
Siderophore Production ◽  
Iron Limitation
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