Cellular localization of cytochemically stained acetylcholinesterase activity in adult rat skeletal muscle

1985 ◽  
Vol 14 (5) ◽  
pp. 795-808 ◽  
Author(s):  
J. Alejandro Donoso ◽  
Hugo L. Fernandez
Keyword(s):  
Skeletal Muscle ◽  
Cellular Localization ◽  
Acetylcholinesterase Activity ◽  
Rat Skeletal Muscle ◽  
Adult Rat

Stable incorporation of a bacterial gene into adult rat skeletal muscle in vivo

1990 ◽  
Vol 258 (3) ◽  
pp. C578-C581 ◽  
Author(s):  
D. B. Thomason ◽  
F. W. Booth
Keyword(s):  
Skeletal Muscle ◽  
Leukemia Virus ◽  
Rat Skeletal Muscle ◽  
Bacterial Gene ◽  
Muscle Gene Expression ◽  
Adult Rat ◽  
Foreign Genes ◽  
Muscle Gene ◽  
Beta Galactosidase

We have developed a novel technique to incorporate and stably express foreign genes in adult rat skeletal muscle in vivo. Endogeneous satellite cells in skeletal muscle regenerating from bupivacaine damage were infected with an injected retrovirus containing the Escherichia coli beta-galactosidase gene under the promoter control of the Moloney murine leukemia virus long-terminal repeat. Constitutive and stable expression of beta-galactosidase activity was observed in muscle fibers after 6 days and 1 mo of muscle regeneration. Two patterns of expression were observed, diffuse expression within fibers and focal expression associated with the sarcolemma. This technique will allow future experiments with muscle-specific genes and promoters to study the physiological regulation of skeletal muscle gene expression in the intact adult mammal. Furthermore, the technique of stimulating stem cell proliferation to allow retroviral-mediated gene transfer may be generally applicable to other tissues.

scholarly journals Glutamatergic Reinnervation and Assembly of Glutamatergic Synapses in Adult Rat Skeletal Muscle Occurs at Cholinergic Endplates

2009 ◽  
Vol 68 (10) ◽  
pp. 1103-1115 ◽  
Author(s):  
Maura Francolini ◽  
Giorgio Brunelli ◽  
Ilaria Cambianica ◽  
Sergio Barlati ◽  
Alessandro Barbon ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Rat Skeletal Muscle ◽  
Glutamatergic Synapses ◽  
Adult Rat

Insulin increases the Na(+)-K(+)-ATPase alpha 2-subunit in the surface of rat skeletal muscle: morphological evidence

1993 ◽  
Vol 265 (6) ◽  
pp. C1716-C1722 ◽  
Author(s):  
A. Marette ◽  
J. Krischer ◽  
L. Lavoie ◽  
C. Ackerley ◽  
J. L. Carpentier ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Plasma Membrane ◽  
Cellular Localization ◽  
Protein A ◽  
Morphological Evidence ◽  
Mammalian Skeletal Muscle ◽  
Tissue Fixation ◽  
Insulin Stimulation ◽  
Rat Skeletal Muscle ◽  
Quantitative Evidence

The cellular localization of the alpha 2-subunit of the Na(+)-K(+)-ATPase was defined by immunoelectron microscopy, and the effect of insulin on the amount of alpha 2-immunoreactive subunits on the cell surface was quantitated. Two protocols were used for tissue fixation and immunolocalization. Protocol 1 was characterized by fixation with 2% paraformaldehyde, use of a monoclonal antibody, and detection with 3-nm-diameter gold-labeled Fab fragments or 10-nm gold-labeled immunoglobulin G. Protocol 2 was characterized by fixation with 4% paraformaldehyde plus 0.1% glutaraldehyde, use of a polyclonal antibody, and detection with 10-nm gold-labeled protein A. In control muscle, the alpha 2-subunit of the Na(+)-K(+)-ATPase was present at the plasma membrane and in intracellular tubular and vesicular structures located in subsarcolemmal and triadic regions. Acute insulin stimulation increased the number of immunolabeled alpha 2-subunits in the plasma membrane after both fixation protocols. The gain in the plasma membrane ranged from 1.5- to 3.7-fold and was significant at the level of P < 0.005. These results provide morphological quantitative evidence that the alpha 2-subunit of the Na(+)-K(+)-ATPase is present both at the plasma membrane and intracellularly in mammalian skeletal muscle and that insulin acutely increases its abundance in the muscle surface.

scholarly journals Changes in Expression and Cellular Localization of Rat Skeletal Muscle ClC-1 Chloride Channel in Relation to Age, Myofiber Phenotype and PKC Modulation

2020 ◽  
Vol 11 ◽  
Author(s):  
Elena Conte ◽  
Adriano Fonzino ◽  
Antonio Cibelli ◽  
Vito De Benedictis ◽  
Paola Imbrici ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Chloride Channel ◽  
Cellular Localization ◽  
Rat Skeletal Muscle

scholarly journals Concentration of Caveolin-3 at the Neuromuscular Junction in Young and Old Rat Skeletal Muscle Fibers

2003 ◽  
Vol 51 (9) ◽  
pp. 1113-1118 ◽  
Author(s):  
Bruce M. Carlson ◽  
Jean A. Carlson ◽  
Eduard I. Dedkov ◽  
Ian S. McLennan
Keyword(s):  
Skeletal Muscle ◽  
Neuromuscular Junction ◽  
Muscle Fibers ◽  
Normal Aging ◽  
Rat Skeletal Muscle ◽  
Phenotypic Characteristics ◽  
Adult Rat ◽  
Rat Muscle ◽  
Functional Implications ◽  
Old Rats

Caveolin-3, a muscle-specific member of the caveolin family, is strongly localized to the neuromuscular junction (NMJ) in adult rat muscle fibers, where it co-localizes with α-bungarotoxin staining. In 24-month-old rats, less distinct staining corresponds with the normal aging changes in the NMJ. After denervation, the pattern and intensity of staining begin to break up as early as 3 days, and by 10 days little staining remains. The functional implications of this concentration of caveolin-3 at the NMJ remain obscure, but it is possible that its absence could account for some of the phenotypic characteristics of individuals with caveolin-3 mutations.

Dihydropyridine receptor isoform expression in adult rat skeletal muscle

1998 ◽  
Vol 436 (3) ◽  
pp. 309-314 ◽  
Author(s):  
Yann Péréon ◽  
Christine Dettbarn ◽  
Ying Lu ◽  
Karin N. Westlund ◽  
Jian-Ting Zhang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Dihydropyridine Receptor ◽  
Rat Skeletal Muscle ◽  
Adult Rat ◽  
Isoform Expression
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