Scanning and transmission electron microscopic studies of microvascular pathology in the osmotically impaired blood-brain barrier

1995 ◽  
Vol 24 (10) ◽  
pp. 795-806 ◽  
Author(s):  
A. S. Lossinsky ◽  
A. W. Vorbrodt ◽  
H. M. Wisniewski
1973 ◽  
Vol 59 (3) ◽  
pp. 711-724
Author(s):  
J. E. TREHERNE

1. The effects of hypertonic urea and hypertonic glucose solutions upon the ‘blood-brain barrier’ in the isolated abdominal nerve cord of the cockroach have been studied. 2. Electrophysiological studies showed that a hypertonic solution of urea, but not of glucose, was effective in reducing the barrier to the entry of potassium and the loss of sodium. 3. Electron-microscopic studies revealed no significant ultrastructural changes, and no disruption of the perineurial barrier to the entry of ionic lanthanum, following comparable exposure to the hypertonic solutions. 4. It is suggested that this alteration of the blood-brain barrier may result from a selective change in permeability of the perineurial membranes or tight junctions.


Author(s):  
M.G. Hamilton ◽  
T.T. Herskovits ◽  
J.S. Wall

The hemocyanins of molluscs are aggregates of a cylindrical decameric subparticle that assembles into di-, tri-, tetra-, penta-, and larger multi-decameric particles with masses that are multiples of the 4.4 Md decamer. Electron micrographs of these hemocyanins typically show the particles with two profiles: circular representing the cylinder viewed from the end and rectangular representing the side-view of the hollow cylinder.The model proposed by Mellema and Klug from image analysis of a didecameric hemocyanin with the two decamers facing one another with collar (closed) ends outward fits the appearance of side-views of the negatively-stained cylinders. These authors also suggested that there might be caps at the ends. In one of a series of transmission electron microscopic studies of molluscan hemocyanins, Siezen and Van Bruggen supported the Mellema-Klug model, but stated that they had never observed a cap component. With STEM we have tested the end cap hypothesis by direct mass measurements across the end-views of unstained particles.


2000 ◽  
Vol 39 (Part 1, No. 6A) ◽  
pp. 3361-3365 ◽  
Author(s):  
Junko Shibata ◽  
Katsuya Yamagiwa ◽  
Izumi Hirabayashi ◽  
Tsukasa Hirayama ◽  
Yuichi Ikuhara

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