In vitro genotoxic effects of areca nut extract and arecoline

1992 ◽  
Vol 118 (4) ◽  
pp. 283-288 ◽  
Author(s):  
Bhavana J. Dave ◽  
Amit H. Trivedi ◽  
Siddharth G. Adhvaryu
Keyword(s):  
Areca Nut ◽  
Genotoxic Effects

Evaluation of genotoxic and non-genotoxic effects of organic air pollution using in vitro bioassays

2007 ◽  
Vol 33 (7) ◽  
pp. 859-866 ◽  
Author(s):  
M. Škarek ◽  
J. Janošek ◽  
P. Čupr ◽  
J. Kohoutek ◽  
A. Novotná-Rychetská ◽  
...  
Keyword(s):  
Air Pollution ◽  
Genotoxic Effects ◽  
In Vitro Bioassays

scholarly journals Evaluation of cytotoxicity and mutagenicity of the benzodiazepine flunitrazepam in vitro and in vivo

2014 ◽  
Vol 50 (2) ◽  
pp. 251-256
Author(s):  
Igor Vivian de Almeida ◽  
Giovana Domingues ◽  
Lilian Capelari Soares ◽  
Elisângela Düsman ◽  
Veronica Elisa Pimenta Vicentini
Keyword(s):  
Rattus Norvegicus ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Term Treatment ◽  
Genotoxic Effects ◽  
Short Term ◽  
Short Term Treatment ◽  
Chromosomal Aberration Test

Flunitrazepam (FNZ) is a sedative benzodiazepine prescribed for the short-term treatment of insomnia. However, there are concerns regarding possible carcinogenic or genotoxic effects of this medicine. Thus, the aim of this study was to evaluate the cytotoxic, clastogenic and aneugenic effects of FNZ in hepatoma cells from Rattus norvegicus (HTC) in vitro and in bone marrow cells of Wistar rats in vivo. These effects were examined in vitro following treatment with 0.2, 1.0, 5.0 or 10 μg/mL FNZ using a micronucleus test with a cytokinesis block or in vivo using a chromosomal aberration test following treatment with 7, 15 or 30 μg/mL/kg body weight. The results showed that the benzodiazepine concentrations tested were not cytotoxic, aneugenic or clastogenic. However, considering the adverse effects of using this benzodiazepine, more studies are required.

scholarly journals Analytical in vitro approach for studying cyto- and genotoxic effects of particulate airborne material

2011 ◽  
Vol 401 (10) ◽  
pp. 3213-3220 ◽  
Author(s):  
Michaela Aufderheide ◽  
Stefanie Scheffler ◽  
Niklas Möhle ◽  
Beat Halter ◽  
Dieter Hochrainer
Keyword(s):  
Genotoxic Effects

scholarly journals In vitro assessment of cytotoxic, apoptotic and genotoxic effects of metformin

2021 ◽  
Vol 51 (2) ◽  
pp. 167-174
Author(s):  
Tuba Sarıaydın ◽  
Tuğbagül Çal ◽  
Sevtap Aydın Dilsiz ◽  
Hande Canpınar ◽  
Ülkü Ündeğer Bucurgat
Keyword(s):  
Genotoxic Effects ◽  
In Vitro Assessment

scholarly journals Genotoxic potential of nonsteroidal hormones

2015 ◽  
Vol 69 (3-4) ◽  
pp. 245-258
Author(s):  
Dijana Topalovic ◽  
Lada Zivkovic ◽  
Ninoslav Djelic ◽  
Vladan Bajic ◽  
Andrea Cabarkapa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Genetic Material ◽  
Experimental Studies ◽  
Genotoxic Effect ◽  
Common Mechanism ◽  
Genotoxic Effects ◽  
Specific Expression ◽  
Study Results

Hormones are cellular products involved in the regulation of a large number of processes in living systems, and which by their actions affect the growth, function and metabolism of cells. Considering that hormones are compounds normally present in the organism, it is important to determine if they can, under certain circumstances, lead to genetic changes in the hereditary material. Numerous experimental studies in vitro and in vivo in different systems, from bacteria to mammals, dealt with the mutagenic and genotoxic effects of hormones. This work presents an overview of the research on genotoxic effects of non?steroidal hormones, although possible changes of genetic material under their influence have not still been known enough, and moreover, investigations on their genotoxic influence have given conflicting results. The study results show that mechanisms of genotoxic effect of nonsteroidal hormones are manifested through the increase of oxidative stress by arising reactive oxygen species. A common mechanism of ROS occurence in thyroid hormones and catecholamines is through metabolic oxidation of their phenolic groups. Manifestation of insulin genotoxic effect is based on production of ROS by activation of NADPH isophorms, while testing oxytocin showed absence of genotoxic effect. Considering that the investigations on genotoxicity of nonsteroidal hormones demonstrated both positive and negative results, the explanation of this discordance involve limitations of test systems themselves, different cell types or biological species used in the experiments, different level of reactivity in vitro and in vivo, as well as possible variations in a tissue-specific expression. Integrated, the provided data contribute to better understanding of genotoxic effect of nonsteroidal hormones and point out to the role and mode of action of these hormones in the process of occurring of effects caused by oxidative stress.

In vitro potential genotoxic effects of surface drinking water treated with chlorine and alternative disinfectants

2004 ◽  
Vol 564 (2) ◽  
pp. 179-193 ◽  
Author(s):  
Licia Guzzella ◽  
Silvano Monarca ◽  
Claudia Zani ◽  
Donatella Feretti ◽  
Ilaria Zerbini ◽  
...  
Keyword(s):  
Drinking Water ◽  
Genotoxic Effects

Evaluation of genotoxic effects in human peripheral blood leukocytes following an acute in vitro exposure to 900 MHz radiofrequency fields

2005 ◽  
Vol 26 (4) ◽  
pp. 258-265 ◽  
Author(s):  
O. Zeni ◽  
M. Romanò ◽  
A. Perrotta ◽  
M.B. Lioi ◽  
R. Barbieri ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Genotoxic Effects ◽  
Blood Leukocytes ◽  
In Vitro Exposure

scholarly journals Cytotoxic, Genotoxic, and Polymorphism Effects on Vanilla planifolia Jacks ex Andrews after Long-Term Exposure to Argovit® Silver Nanoparticles

Nanomaterials ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 754 ◽  
Author(s):  
Jericó Bello-Bello ◽  
José Spinoso-Castillo ◽  
Samantha Arano-Avalos ◽  
Eduardo Martínez-Estrada ◽  
María Arellano-García ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Large Scale ◽  
Inter Simple Sequence Repeat ◽  
Metallic Silver ◽  
Vanilla Planifolia ◽  
Genotoxic Effects ◽  
Total Percentage ◽  
Long Time ◽  
Mexican Government

Worldwide demands of Vanilla planifolia lead to finding new options to produce large-scale and contaminant-free crops. Particularly, the Mexican Government has classified Vanilla planifolia at risk and it subject to protection programs since wild species are in danger of extinction and no more than 30 clones have been found. Nanotechnology could help to solve both demands and genetic variability, but toxicological concerns must be solved. In this work, we present the first study of the cytotoxic and genotoxic effects promoted by AgNPs in Vanilla planifolia plantlets after a very long exposure time of six weeks. Our results show that Vanilla planifolia plantlets growth with doses of 25 and 50 mg/L is favored with a small decrease in the mitotic index. A dose-dependency in the frequency of cells with chromosomal aberrations and micronuclei was found. However, genotoxic effects could be considered as minimum due to with the highest concentration employed (200 mg/L), the total percentage of chromatic aberrations is lower than 5% with only three micronuclei in 3000 cells, despite the long-time exposure to AgNP. Therefore, 25 and 50 mg/L (1.5 and 3 mg/L of metallic silver) were identified as safe concentrations for Vanilla planifolia growth on in vitro conditions. Exposure of plantlets to AgNPs increase the polymorphism registered by inter-simple sequence repeat method (ISSR), which could be useful to promote the genetic variability of this species.

scholarly journals Evaluation of in vitro genotoxic effects induced by in vitro anther culture conditions in sunflower

2019 ◽  
Vol 14 (9) ◽  
pp. 1633885 ◽  
Author(s):  
Elif Çakmak ◽  
Ahu Altınkut Uncuoğlu ◽  
Yıldız Aydın
Keyword(s):  
Anther Culture ◽  
Culture Conditions ◽  
Genotoxic Effects
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