S19.10 Chemical structure and serological specificity of proteus mirabilis 028 O-specific polysaccharide and lipopolysaccharide from a transient form of proteus mirabilis R14/1959

Professor Krystyna Kotełko was working as a microbiologist at the University of Łódź (Poland). Her main object of study was the LPS (endotoxin) of opportunistic urinary pathogens from the genus Proteus. She demonstrated, for the first time, the presence of uronic acids and amino acids, as well as two heptoses (L- glycero-D- manno-heptose and D- glycero-D- manno-heptose) and hexosamines in Proteus LPS, and developed a classification scheme of the Proteus LPS into chemotypes. Prof Kotełko also initiated studies on the chemical structure of Proteus O-specific polysaccharide and investigations on the serological specificity of this part of LPS, as well its core region. She also analysed the virulence factors of these bacteria, such as haemolysin and invasiveness.

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The Chemical Structure of the Lipopolysaccharide of a Rc-Type Mutant of Proteus mirabilis Lacking 4-Amino-4-Deoxy-L Arabinose and Its Susceptibility towards Polymyxin B

Endotoxin - Advances in Experimental Medicine and Biology ◽

10.1007/978-1-4757-5140-6_7 ◽

1990 ◽

pp. 121-126

Author(s):

J. Radziejewska-Lebrecht ◽

U. R. Bhat ◽

H. Brade ◽

W. Kaca ◽

H. Mayer

Keyword(s):

Proteus Mirabilis ◽

Chemical Structure ◽

Polymyxin B ◽

Type Mutant

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Structure of the O-specific polysaccharide of Proteus mirabilis D52 and typing of this strain to Proteus serogroup O33

European Journal of Biochemistry ◽

10.1046/j.1432-1327.2001.02356.x ◽

2001 ◽

Vol 268 (15) ◽

pp. 4346-4351 ◽

Cited By ~ 49

Author(s):

Krystyna Zych ◽

Filip V. Toukach ◽

Nikolay P. Arbatsky ◽

Katarzyna Kołodziejska ◽

Sof'ya N. Senchenkova ◽

...

Keyword(s):

Proteus Mirabilis ◽

Specific Polysaccharide

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Structure of the O-specific polysaccharide of Proteus mirabilis O11, another Proteus O-antigen containing an amide of d-galacturonic acid with l-threonine

Carbohydrate Research ◽

10.1016/s0008-6215(99)00257-8 ◽

1999 ◽

Vol 323 (1-4) ◽

pp. 81-86 ◽

Cited By ~ 14

Author(s):

Nikolay P Arbatsky ◽

Alexander S Shashkov ◽

Elzbieta Literacka ◽

Göran Widmalm ◽

Wieslaw Kaca ◽

...

Keyword(s):

Proteus Mirabilis ◽

Galacturonic Acid ◽

O Antigen ◽

Specific Polysaccharide

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Interferon Production by Nonviral Stimuli of Microbial Origin

The Journal of General Physiology ◽

10.1085/jgp.56.1.25 ◽

1970 ◽

Vol 56 (1) ◽

pp. 25-40 ◽

Cited By ~ 4

Author(s):

Julius S. Youngner

Keyword(s):

Chemical Structure ◽

Bacterial Endotoxin ◽

Interferon Response ◽

Gram Negative Bacteria ◽

Interferon Production ◽

Protein Synthesis Inhibitors ◽

Microbial Origin ◽

Current State ◽

Active Moiety ◽

Specific Polysaccharide

An increasing number of nonviral materials of microbial origin has been reported to stimulate the production of interferon in cell cultures and (or) in animals. These materials include (a) gram-negative bacteria or the endotoxins prepared from their cell walls, (b) other microorganisms such as Rickettsiae, Bedsoniae, Protozoa, and (c) fungal products such as a mannan from Candida and various antibiotics which act as protein synthesis inhibitors, e.g., glutarimide antibiotics and tenuazonic acid. A summary is presented of the current state of knowledge about interferon production in animals by the most thoroughly studied nonviral substance of microbial origin, bacterial endotoxin. Further evidence is presented which clearly distinguishes the "endotoxin-type" of interferon response in animals from the response seen after the injection of virus. The data suggest that the release of preformed interferon from the tissues occurs in animals injected with endotoxin. On the other hand, interferon produced in response to the injection of virus is newly synthesized protein. While the exact chemical structure of the component of bacterial endotoxin responsible for interferon release has not yet been elucidated, it is clear that the lipid portion of the lipopolysaccharide, rather than the O-specific polysaccharide side chains or the core polysaccharide, is the active moiety.

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The structure of Proteus mirabilis O3 O-specific polysaccharide containing N-(2-hydroxyethyl)-D-alanine

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1990.tb15446.x ◽

1990 ◽

Vol 188 (3) ◽

pp. 645-651 ◽

Cited By ~ 22

Author(s):

Evgeny V. VINOGRADOV ◽

Wieslaw KACA ◽

Alexander S. SHASHKOV ◽

Danuta KRAJEWSKA-PIETRASIK ◽

Antoni ROZALSKI ◽

...

Keyword(s):

Proteus Mirabilis ◽

Specific Polysaccharide

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Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rifr and Study of Its Immunomodulatory Activity

Marine Drugs ◽

10.3390/md19110646 ◽

2021 ◽

Vol 19 (11) ◽

pp. 646

Author(s):

Rossella Di Guida ◽

Angela Casillo ◽

Antonietta Stellavato ◽

Celeste Di Meo ◽

Soichiro Kawai ◽

...

Keyword(s):

Chemical Structure ◽

Lipid A ◽

Membrane Integrity ◽

Immunomodulatory Activity ◽

Gram Negative Bacteria ◽

Core Oligosaccharide ◽

Gram Negative ◽

Seriola Quinqueradiata ◽

Specific Polysaccharide

Lipopolysaccharides (LPS) are surface glycoconjugates embedded in the external leaflet of the outer membrane (OM) of the Gram-negative bacteria. They consist of three regions: lipid A, core oligosaccharide (OS), and O-specific polysaccharide or O-antigen. Lipid A is the glycolipid endotoxin domain that anchors the LPS molecule to the OM, and therefore, its chemical structure is crucial in the maintenance of membrane integrity in the Gram-negative bacteria. In this paper, we reported the characterization of the lipid A and OS structures from Pseudoalteromonas nigrifaciens Sq02-Rifr, which is a psychrotrophic Gram-negative bacterium isolated from the intestine of Seriola quinqueradiata. The immunomodulatory activity of both LPS and lipid A was also examined.

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CHEMO-IMMUNOLOGICAL STUDIES ON CONJUGATED CARBOHYDRATE-PROTEINS

Journal of Experimental Medicine ◽

10.1084/jem.60.1.85 ◽

1934 ◽

Vol 60 (1) ◽

pp. 85-94 ◽

Cited By ~ 8

Author(s):

Walther F. Goebel ◽

Frank H. Babers ◽

Oswald T. Avery

Keyword(s):

Chemical Structure ◽

Immunological Properties ◽

Serological Specificity

The chemical and immunological properties of the acetylated and unacetylated forms of the p-aminophenol ß-glucoside of glucose have been described. The serological specificity of these ß-glucosides in combination with protein has been correlated with known changes in chemical structure and has been compared with the immunological properties of the α-glucoside of the same hexose.

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