A fluorescent antibody technique for the detection of the group-specific antigen of feline leukemia virus in infected tissue-culture cells

Virology ◽  
1971 ◽  
Vol 44 (1) ◽  
pp. 219-222 ◽  
Author(s):  
Tito Ubertini ◽  
Fernando Noronha ◽  
John E. Post ◽  
Charles G. Rickard
Keyword(s):  
Tissue Culture ◽  
Leukemia Virus ◽  
Fluorescent Antibody ◽  
Specific Antigen ◽  
Infected Tissue ◽  
Feline Leukemia Virus ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Culture Cells ◽  
Tissue Culture Cells

scholarly journals SEQUENTIAL CELLULAR CHANGES PRODUCED BY TYPES 5 AND 7 ADENOVIRUSES IN HELA CELLS AND IN HUMAN AMNIOTIC CELLS

1959 ◽  
Vol 110 (5) ◽  
pp. 827-844 ◽  
Author(s):  
Georgiana S. Boyer ◽  
Floyd W. Denny ◽  
Harold S. Ginsberg
Keyword(s):  
Tissue Culture ◽  
Cell Nucleus ◽  
Hela Cells ◽  
Fluorescent Antibody ◽  
Intranuclear Inclusions ◽  
Culture Cells ◽  
Cellular Changes ◽  
Tissue Culture Cells ◽  
Cytological Changes ◽  
Amniotic Cells

The sequential cytological changes which develop in tissue culture cells infected with adenovirus types 5 and 7 are described and compared with those produced by adenovirus types 1, 2, 3, and 4. The evidence that is presented indicates that types 1, 2, and 5 belong to one major subdivision of the adenovirus group and types 3, 4, and 7 to another. That the host cell nucleus is the principal site of adenovirus synthesis has been confirmed by fluorescent antibody studies. They have demonstrated the occurrence of type-specific adenovirus antigen in the characteristic intranuclear inclusions and other virus-induced structures reported to contain virus-like particles or shown by electronmicroscopy.

scholarly journals Simultaneous localization of myosin and tubulin in human tissue culture cells by double antibody staining

1978 ◽  
Vol 77 (1) ◽  
pp. 182-195 ◽  
Author(s):  
K Fujiwara ◽  
TD Pollard
Keyword(s):  
Tissue Culture ◽  
Protein Interactions ◽  
Single Cells ◽  
Fluorescent Antibody ◽  
Interphase Cell ◽  
Culture Cells ◽  
Antibody Staining ◽  
Tissue Culture Cells ◽  
Early Anaphase ◽  
Fluorescence Contrast

We have studied the distribution of myosin and tubulin molecules inside the same tissue culture cells by using two antibodies labeled with contrasting fluorochromes. Antimyosin raised against human platelet myosin was labeled with rhodamine. Antitubulin raised against sea urchin vinblastine-induced tubulin crystals was labeled with fluorescein. The two antibodies stained entirely different structures inside the same flat interphase cell: antimyosin bound to stress fibers and antitubulin bound to thin, wavy fibers thought to be individual microtubules. Compact interphase cells stained diffusely with both antibodies. From prophase through early anaphase both antibodies stained the mitotic spindle, although the fluorescence contrast between the spindle and the cytoplasm was much higher with antitubulin than with antimyosin. From anaphase through telophase, strong antimyosin staining occurred in the cleavage furrow, while antitubulin stained the region between the separated chromosomes. This study established the feasibility of high-resolution fluorescent antibody localization of pairs of motility proteins in the cytoplasm of single cells, an approach which will make it possible to map out the sites of the various contractile protein interactions in situ.

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