Electron microscopical demonstration of acid phosphatase activity in the central nervous system

1966 ◽  
Vol 6 (3) ◽  
pp. 254-259 ◽  
Author(s):  
Georg W. Kreutzberg ◽  
Hermann Hager
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
The Central Nervous System ◽  
Electron Microscopical

scholarly journals Acid-beta-glycerophosphatase reaction products in the central nervous system mitochondria following x-ray irradiation.

1975 ◽  
Vol 23 (6) ◽  
pp. 402-410 ◽  
Author(s):  
L Roizin ◽  
D Orlovskaja ◽  
J C Liu ◽  
A L Carsten
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Acid Phosphatase ◽  
Enzyme Reaction ◽  
Reaction Products ◽  
Huntington's Chorea ◽  
X Ray ◽  
System A ◽  
Chemical Lesion ◽  
The Central Nervous System

A survey of the literature to date on the enzyme histochemistry of intracellular organelles has not yielded any reference to the presence of acid phosphatase reaction products in the mammalian mitochondria of the central nervous system. A combination of Gomori's acid phosphatase mehtod, however, with standard electron microscopy has disclosed the presence of enzyme reaction products in the mitochondria of the central nervous system of rats from 2 hr to 22 weeks after x-ray irradiation, as well as in a cerebral biopsy performed on a patient affected by Huntington's chorea. No enzyme reaction products, on the other hand, were observed in serial sections that had been incubated in substrates either containing sodium fluoride or lacking in beta-glycerophosphate. The abnormal mitochondrial enzyme reaction (chemical lesion) is considered to be the consequenco of the pathologic process affecting the ultrastructural-chemical organization of the organelle.

Acid Phosphatase Activity in the Neutral Red Granules of Mouse Exocrine Pancreas Cells

1964 ◽  
Vol s3-105 (71) ◽  
pp. 343-348
Author(s):  
JENNIFER M. BYRNE
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Exocrine Pancreas ◽  
Neutral Red ◽  
Pancreatic Tissue ◽  
Microscopical Level ◽  
Electron Microscopical Level ◽  
Electron Microscopical

The Gomori test for acid phosphatase was performed on pancreatic tissue from mice injected with neutral red. On an electron microscopical level, acid phosphatase activity was found to be localized in the neutral red granules. It is suggested that the neutral red granules are of lysosomal derivation.

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