Acceleration of desipramine-induced changes on the dopamine receptor-coupled adenylate cyclase system by pertussis toxin

1994 ◽  
Vol 98 (2) ◽  
pp. 133-142 ◽  
Author(s):  
F. Okada ◽  
N. Takahashi ◽  
A. Ito ◽  
Y. Tokumitsu ◽  
Y. Nomura
Keyword(s):  
Adenylate Cyclase ◽  
Dopamine Receptor ◽  
Pertussis Toxin ◽  
Adenylate Cyclase System ◽  
Induced Changes

Muscarinic receptors in MDCK cells are coupled to multiple messenger systems

1992 ◽  
Vol 263 (6) ◽  
pp. C1289-C1294 ◽  
Author(s):  
D. Mohuczy-Dominiak ◽  
L. C. Garg
Keyword(s):  
Adenylate Cyclase ◽  
Muscarinic Receptors ◽  
Pertussis Toxin ◽  
Radioligand Binding ◽  
Mdck Cells ◽  
Adenylate Cyclase System ◽  
Dependent Manner ◽  
Muscarinic Antagonists ◽  
Binding Studies ◽  
Camp Formation

Our studies on Madin-Darby canine kidney (MDCK) cells have demonstrated that high-affinity specific muscarinic receptors coupled to the phosphoinositide system are present in these cells. To determine whether muscarinic receptors in MDCK cells are linked negatively to the adenylate cyclase system, we measured the effect of muscarinic agonists and antagonists on vasopressin-, isoproterenol-, and forskolin-stimulated adenosine 3',5'-cyclic monophosphate (cAMP) formation. Vasopressin produced a maximum stimulation of cAMP formation of 13 pmol.10(6) cells-1.2 min-1 at 10(-7) M. Isoproterenol and forskolin stimulated cAMP formation production to 21 pmol.10(6) cells-1.2 min-1 and 64 pmol.10(6) cells-1.10 min-1, respectively, at 10(-4) M. The effects of vasopressin, isoproterenol, and forskolin were blocked by arecoline, a cholinergic agonist, in a concentration-dependent manner. The arecoline response was blocked by treatment of the cells with pertussis toxin. The inhibition by arecoline of forskolin-stimulated cAMP formation was reversed by various muscarinic antagonists in the following order of potency: 4-diphenyl-acetoxy-N-methylpiperidine > p-fluorohexahydrosiladifenidol > pirenzepine > methoctramine. This order of potency of muscarinic antagonists is similar to that observed in our radioligand binding studies and is consistent with the M3 subtype of muscarinic receptors. Our results indicate that muscarinic receptors in MDCK cells are coupled negatively to the adenylate cyclase system via pertussis toxin-sensitive G protein. It is concluded that this intracellular system may at least be partially responsible for the action of cholinergic agonists in these cells and in the kidney.

Effects of ceruletide on perioral movements and the dopamine receptor-adenylate cyclase system in rats chronically treated with fluphenazine

1996 ◽  
Vol 125 (3) ◽  
pp. 185-194 ◽  
Author(s):  
T. Ashizawa ◽  
T. Saito ◽  
N. Takahata
Keyword(s):  
Adenylate Cyclase ◽  
Dopamine Receptor ◽  
Adenylate Cyclase System

Pertussis Toxin-Catalyzed ADP-Ribosylation: Effects on the Coupling of Inhibitory Receptors to the Adenylate Cyclase System

1984 ◽  
Vol 4 (1-6) ◽  
pp. 459-474 ◽  
Author(s):  
Joel Moss ◽  
Paola Bruni ◽  
Judith A. Hsia ◽  
Su-Chen Tsai ◽  
Paul A. Watkins ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Pertussis Toxin ◽  
Adenylate Cyclase System ◽  
Inhibitory Receptors ◽  
Adp Ribosylation

Effects of ceruletide on the dopamine receptor-adenylate cyclase system in striatum and frontal cortex of rats chronically treated with haloperidol

1993 ◽  
Vol 110 (4) ◽  
pp. 383-389 ◽  
Author(s):  
Yuko Hatta ◽  
Shinichi Hatta ◽  
Toshikazu Saito
Keyword(s):  
Adenylate Cyclase ◽  
Frontal Cortex ◽  
Dopamine Receptor ◽  
Adenylate Cyclase System

scholarly journals D-2 dopamine receptor-mediated inhibition of pro-opiomelanocortin synthesis in rat intermediate lobe. Abolition by pertussis toxin or activators of adenylate cyclase.

1986 ◽  
Vol 261 (10) ◽  
pp. 4555-4561
Author(s):  
T E Cote ◽  
R Felder ◽  
J W Kebabian ◽  
R D Sekura ◽  
T Reisine ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Dopamine Receptor ◽  
Pertussis Toxin ◽  
Intermediate Lobe

Effects of pertussis toxin on the α2adrenoceptor-inhibitory GTP-binding protein-adenylate cyclase system in rat brain: pharmacological and neurochemical studies

1987 ◽  
Vol 134 (2) ◽  
pp. 123-129 ◽  
Author(s):  
Yasuyuki Nomura ◽  
Keiko Kawata ◽  
Yoshihisa Kitamura ◽  
Hiroshi Watanabe
Keyword(s):  
Adenylate Cyclase ◽  
Rat Brain ◽  
Pertussis Toxin ◽  
Binding Protein ◽  
Adenylate Cyclase System ◽  
Gtp Binding Protein ◽  
Gtp Binding
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