Modulation of responsiveness of the adenylate cyclase system in avian chondroprogenitor cells by pertussis toxin, PTH, and PGE2

2009 ◽  
Vol 4 (5) ◽  
pp. 743-750 ◽  
Author(s):  
Mark Pines ◽  
Bernard Yosif ◽  
Shmuel Hurwitz
Keyword(s):  
Adenylate Cyclase ◽  
Pertussis Toxin ◽  
Adenylate Cyclase System ◽  
Chondroprogenitor Cells

Muscarinic receptors in MDCK cells are coupled to multiple messenger systems

1992 ◽  
Vol 263 (6) ◽  
pp. C1289-C1294 ◽  
Author(s):  
D. Mohuczy-Dominiak ◽  
L. C. Garg
Keyword(s):  
Adenylate Cyclase ◽  
Muscarinic Receptors ◽  
Pertussis Toxin ◽  
Radioligand Binding ◽  
Mdck Cells ◽  
Adenylate Cyclase System ◽  
Dependent Manner ◽  
Muscarinic Antagonists ◽  
Binding Studies ◽  
Camp Formation

Our studies on Madin-Darby canine kidney (MDCK) cells have demonstrated that high-affinity specific muscarinic receptors coupled to the phosphoinositide system are present in these cells. To determine whether muscarinic receptors in MDCK cells are linked negatively to the adenylate cyclase system, we measured the effect of muscarinic agonists and antagonists on vasopressin-, isoproterenol-, and forskolin-stimulated adenosine 3',5'-cyclic monophosphate (cAMP) formation. Vasopressin produced a maximum stimulation of cAMP formation of 13 pmol.10(6) cells-1.2 min-1 at 10(-7) M. Isoproterenol and forskolin stimulated cAMP formation production to 21 pmol.10(6) cells-1.2 min-1 and 64 pmol.10(6) cells-1.10 min-1, respectively, at 10(-4) M. The effects of vasopressin, isoproterenol, and forskolin were blocked by arecoline, a cholinergic agonist, in a concentration-dependent manner. The arecoline response was blocked by treatment of the cells with pertussis toxin. The inhibition by arecoline of forskolin-stimulated cAMP formation was reversed by various muscarinic antagonists in the following order of potency: 4-diphenyl-acetoxy-N-methylpiperidine > p-fluorohexahydrosiladifenidol > pirenzepine > methoctramine. This order of potency of muscarinic antagonists is similar to that observed in our radioligand binding studies and is consistent with the M3 subtype of muscarinic receptors. Our results indicate that muscarinic receptors in MDCK cells are coupled negatively to the adenylate cyclase system via pertussis toxin-sensitive G protein. It is concluded that this intracellular system may at least be partially responsible for the action of cholinergic agonists in these cells and in the kidney.

Pertussis Toxin-Catalyzed ADP-Ribosylation: Effects on the Coupling of Inhibitory Receptors to the Adenylate Cyclase System

1984 ◽  
Vol 4 (1-6) ◽  
pp. 459-474 ◽  
Author(s):  
Joel Moss ◽  
Paola Bruni ◽  
Judith A. Hsia ◽  
Su-Chen Tsai ◽  
Paul A. Watkins ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Pertussis Toxin ◽  
Adenylate Cyclase System ◽  
Inhibitory Receptors ◽  
Adp Ribosylation

Effects of pertussis toxin on the α2adrenoceptor-inhibitory GTP-binding protein-adenylate cyclase system in rat brain: pharmacological and neurochemical studies

1987 ◽  
Vol 134 (2) ◽  
pp. 123-129 ◽  
Author(s):  
Yasuyuki Nomura ◽  
Keiko Kawata ◽  
Yoshihisa Kitamura ◽  
Hiroshi Watanabe
Keyword(s):  
Adenylate Cyclase ◽  
Rat Brain ◽  
Pertussis Toxin ◽  
Binding Protein ◽  
Adenylate Cyclase System ◽  
Gtp Binding Protein ◽  
Gtp Binding

scholarly journals Effects of litter removal on the lipolytic response and the regulatory components of the adenylate cyclase in adipocytes isolated from lactating rats

1992 ◽  
Vol 281 (2) ◽  
pp. 333-337 ◽  
Author(s):  
M Ros ◽  
G Alonso ◽  
F J Moreno
Keyword(s):  
Adipose Tissue ◽  
Adenylate Cyclase ◽  
Pertussis Toxin ◽  
Adrenergic Receptors ◽  
Plasma Membranes ◽  
Adenylate Cyclase System ◽  
Litter Removal ◽  
The Status ◽  
Hormone Sensitive ◽  
A1 Adenosine Receptors

The effects of litter removal on the status of different components of the hormone-sensitive adenylate cyclase system were analysed in plasma membranes of rat adipocytes. These effects were correlated with the decreased lipolytic response of adipose tissue. No changes in total number of A1 adenosine receptors or their affinity were detected in response to litter removal. In contrast, beta-adrenergic receptors showed a decrease (35%) in total number of receptors, without any significant change in their affinity. The status of alpha-GS and alpha-Gi, the alpha-subunits of G proteins which mediate stimulation and inhibition respectively of adenylate cyclase, were probed by cholera- and pertussis-toxin-catalysed ADP-ribosylation respectively and by immunoblot. Associated with litter removal, decreases of 63% and 62% in the incorporation of [alpha 32P]ADP-ribose catalysed by cholera toxin and pertussis toxin into alpha-Gs and alpha-Gi respectively were detected. Immunoblotting using RM/1 (anti-alpha-Gs) and AS/7 (anti-alpha-Gi) antisera also showed decreases in the levels of alpha-Gs (52%) and alpha-Gi (55%) in adipocyte membranes from litter-removed rats compared with lactating rats. Alterations in the status of hormone-sensitive adenylate cyclase components, such as those described herein, may be biochemical mechanism(s) by which adipose tissue shows a decreased lipolytic response during recovery from lactation.

Acceleration of desipramine-induced changes on the dopamine receptor-coupled adenylate cyclase system by pertussis toxin

1994 ◽  
Vol 98 (2) ◽  
pp. 133-142 ◽  
Author(s):  
F. Okada ◽  
N. Takahashi ◽  
A. Ito ◽  
Y. Tokumitsu ◽  
Y. Nomura
Keyword(s):  
Adenylate Cyclase ◽  
Dopamine Receptor ◽  
Pertussis Toxin ◽  
Adenylate Cyclase System ◽  
Induced Changes
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