Alteration of cellulose microfibril formation in eukaryotic cells: Calcofluor white interferes with microfibril assembly and orientation inOocystis apiculata

PROTOPLASMA ◽  
1982 ◽  
Vol 113 (1) ◽  
pp. 1-9 ◽  
Author(s):  
E. Roberts ◽  
R. W. Seagull ◽  
C. H. Haigler ◽  
R. M. Brown
Keyword(s):  
Cellulose Microfibril ◽  
Eukaryotic Cells ◽  
Calcofluor White ◽  
Microfibril Assembly ◽  
Microfibril Formation

scholarly journals Calcofluor white and Congo red inhibit chitin microfibril assembly of Poterioochromonas: evidence for a gap between polymerization and microfibril formation.

1980 ◽  
Vol 87 (2) ◽  
pp. 442-450 ◽  
Author(s):  
W Herth
Keyword(s):  
Electron Microscopy ◽  
Plasma Membrane ◽  
Congo Red ◽  
Light And Electron Microscopy ◽  
Calcofluor White ◽  
Microfibril Assembly ◽  
Irregular Networks ◽  
Dependent Inhibition ◽  
Lorica Formation ◽  
Microfibril Formation

The influence of the light microscopical stains, Calcofluor white and Congo red, on the process of chitin microfibril formation of the chrysoflagellate alga Poterioochromonas stipitata was studied with light and electron microscopy. There is a concentration-dependent inhibition of lorica formation with both dyes. In the presence of the inhibitors malformed loricae are made, which do not show the usual ultrastructure and arrangement of the chitin microfibrils. Instead of long, laterally associated microfibrils, short rods or irregular networks of subelementary (15-25 A) fibrils are found. Microfibril assembly obviously takes place on the accessible outside of the plasma membrane. There must be a gap between the polymerization and microfibril formation reactions, allowing the stains to bind to the polymerized subunits. Thus, later association of these units to form microfibrils is disturbed. The microfibril-orienting mechanism also depends on normal microfibril formation. A model summarizing these hypotheses is suggested.

scholarly journals Cellulose Microfibril Formation by Surface-Tethered Cellulose Synthase Enzymes

ACS Nano ◽  
2016 ◽  
Vol 10 (2) ◽  
pp. 1896-1907 ◽  
Author(s):  
Snehasish Basu ◽  
Okako Omadjela ◽  
David Gaddes ◽  
Srinivas Tadigadapa ◽  
Jochen Zimmer ◽  
...  
Keyword(s):  
Cellulose Microfibril ◽  
Cellulose Synthase ◽  
Microfibril Formation

scholarly journals Callose deposition in the primary wall of suspension cells and regenerating protoplasts, and its relationship to patterned cellulose synthesis

1996 ◽  
Vol 74 (7) ◽  
pp. 1040-1049 ◽  
Author(s):  
Ton N. M. van Amstel ◽  
Harry M. P. Kengen
Keyword(s):  
Cellulose Microfibril ◽  
Morinda Citrifolia ◽  
Aniline Blue ◽  
Cellulose Synthesis ◽  
Suspension Cells ◽  
Calcofluor White ◽  
Callose Deposition ◽  
Fluorescent Brightener ◽  
Tobacco Suspension Cells ◽  
Lateral Walls

Monitoring cell-wall formation in vivo with Fluorescent Brightener 28, by fluorescence microscopy, revealed that tobacco protoplasts regeneration started within 30 min indicated by cellulose microfibril formation at distinct sites on the protoplast surface. Oriented cellulose microfibril deposition was apparent before elongation and indicated the early polarization of protoplasts. The sequence of cellulose microfibril deposition correlates with an helicoidal-like texture. Within 6 h, a texture was completed. Tobacco suspension cells, stained by decolourized aniline blue, showed radiant granular callose fluorescence in cell plates and transverse walls. During the culture cycle of suspension cells, transverse fibrillar deposits of callose gradually appeared in the lateral walls during the log-phase, and subsequently disappeared in the early stationary phase of the cell culture. Similar callose transitions were observed in regenerated elongating protoplasts. Culture cells of Morinda citrifolia L. only showed transient granular depositions in the lateral walls. The callose formations did not result from artificial wounding. The transient appearance of callose might be related to cellulose microfibril deposition. Keywords: aniline blue, Calcofluor White ST, callose, cellulose, Fluorescent Brightener 28, Morinda citrifolia, Nicotiana tabacum.

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