Detection of IL-1?, TNF-?, and IL-6 gene transcription by the polymerase chain reaction in keratinocytes, Langerhans cells and peritoneal exudate cells during infection with herpes simplex virus-1

1992 ◽  
Vol 126 (1-4) ◽  
pp. 253-269 ◽  
Author(s):  
E. Sprecher ◽  
Y. Becker
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Gene Transcription ◽  
Langerhans Cells ◽  
Chain Reaction ◽  
Herpes Simplex Virus 1 ◽  
Peritoneal Exudate Cells ◽  
Polymerase Chain ◽  
Simplex Virus

Diagnosis of herpes simplex virus-1 keratitis: Comparison of Giemsa stain, immunofluorescence assay and polymerase chain reaction

2004 ◽  
Vol 29 (2-3) ◽  
pp. 209-213 ◽  
Author(s):  
Shaheen Subhan ◽  
Roby James Jose ◽  
Aparna Duggirala ◽  
R. Hari ◽  
Pravin Krishna ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Immunofluorescence Assay ◽  
Giemsa Stain ◽  
Chain Reaction ◽  
Herpes Simplex Virus 1 ◽  
Polymerase Chain ◽  
Simplex Virus

Diagnosis of Herpes Simplex Virus 1 and 2 in Clinical Specimens by Tissue Culture and Polymerase Chain Reaction

2018 ◽  
Vol 27 (3) ◽  
pp. 97-104
Author(s):  
Laila A. Farid ◽  
Eman I. A. Mahmoud ◽  
Walaa S. Khater ◽  
Marwa K. Assaad ◽  
Aly M. Zaki
Keyword(s):  
Polymerase Chain Reaction ◽  
Tissue Culture ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Chain Reaction ◽  
Herpes Simplex Virus 1 ◽  
Clinical Specimens ◽  
Polymerase Chain ◽  
Simplex Virus

scholarly journals Septic shock complicated by disseminated herpes simplex virus-1 infection: a case report

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Amélie Boquet ◽  
Guillaume Boulay ◽  
Etienne Hautin ◽  
Nicolas Mottard
Keyword(s):  
Polymerase Chain Reaction ◽  
Intensive Care ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Multiorgan Failure ◽  
Chain Reaction ◽  
Herpes Simplex Virus 1 ◽  
Appropriate Treatment ◽  
Polymerase Chain ◽  
Simplex Virus

Abstract Background Herpes virus remains dormant in human cells and could reactivate under immunosuppressed conditions, such as prolonged critical illnesses. The phenomenon of viral replication during intensive care is well known, even in patients without a history of immunosuppression, but it usually does not have a clinical impact. Systemic reactivation leads to viral DNA in blood. It remains unclear whether this replication is a marker of morbimortality or a true pathogenic process. Therefore, it is unclear what medical treatment is most appropriate for simple replication. In organ damage suspected to be induced by herpes virus, there is no consensus on the most appropriate treatment duration. Here, we report a rarely described case of multiorgan failure implicating herpes simplex virus and discuss its treatment. Case report A 53-year-old Caucasian immunosuppressed woman was admitted to the intensive care unit for septic shock. She presented pneumonia due to Klebsiella pneumoniae. Two weeks after admission, she showed multiorgan failure with acute respiratory distress syndrome and circulation failure. She had digestive and cutaneous lesions typical of herpes simplex virus 1. Blood and respiratory polymerase chain reaction was strongly herpes simplex virus-1 positive. No other bacteria, fungi, or viruses were found. The evolution was rapidly favorable after the initiation of antiviral treatment. Treatment was stopped after 3 weeks of well-conducted antiviral therapy. Curative-dose treatment was interrupted despite continuous strongly positive blood polymerase chain reaction results. In this context, prophylactic treatment was continued. Conclusion We report an exceptional presentation of multiorgan failure in the intensive care unit due to herpes simplex virus-1. The diagnosis was made based on typical herpes simplex virus-1 visceral lesions and the absence of other responsible microorganisms. Intense viral replication is a key diagnostic element. There is no consensus regarding the most appropriate treatment duration, but such decisions should not be based on blood polymerase chain reaction.

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