scholarly journals Standardization of an in-house multiplex real-time polymerase chain reaction for the simultaneous detection of Toxoplasma gondii, Rubella virus, cytomegalovirus, herpes simplex Virus 1 and 2, and Treponema pallidum infection among pregnant women

2021 ◽  
Vol 65 (4) ◽  
pp. 369
Author(s):  
Mageshbabu Ramamurthy ◽  
Prashanth Rajendiran ◽  
Nithiyanandan Saravanan ◽  
Sathish Sankar ◽  
Rajasekar Aruliah ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Toxoplasma Gondii ◽  
Simultaneous Detection ◽  
Treponema Pallidum ◽  
Chain Reaction ◽  
Herpes Simplex Virus 1 ◽  
Polymerase Chain ◽  
Simplex Virus

scholarly journals APPLICATION OF POLYMERASE CHAIN REACTION TO DIFFERENTIATE HERPES SIMPLEX VIRUS 1 AND 2 SEROTYPES IN CULTURE NEGATIVE INTRAOCULAR ASPIRATES

2005 ◽  
Vol 23 (4) ◽  
pp. 239-244
Author(s):  
G Shyamala ◽  
P Sowmya ◽  
B Sudha ◽  
J Malathi ◽  
LK Therese ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Chain Reaction ◽  
Herpes Simplex Virus 1 ◽  
Polymerase Chain ◽  
Simplex Virus ◽  
Culture Negative

Diagnosis of herpes simplex virus-1 keratitis: Comparison of Giemsa stain, immunofluorescence assay and polymerase chain reaction

2004 ◽  
Vol 29 (2-3) ◽  
pp. 209-213 ◽  
Author(s):  
Shaheen Subhan ◽  
Roby James Jose ◽  
Aparna Duggirala ◽  
R. Hari ◽  
Pravin Krishna ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Immunofluorescence Assay ◽  
Giemsa Stain ◽  
Chain Reaction ◽  
Herpes Simplex Virus 1 ◽  
Polymerase Chain ◽  
Simplex Virus

Development of a multiplex polymerase chain reaction for detection and typing of major human herpesviruses in cerebrospinal fluid

2007 ◽  
Vol 53 (10) ◽  
pp. 1117-1122 ◽  
Author(s):  
Massimiliano Bergallo ◽  
Cristina Costa ◽  
Samuela Margio ◽  
Francesca Sidoti ◽  
Maria Elena Terlizzi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Viral Infections ◽  
Simultaneous Detection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Simplex Virus ◽  
Human Herpesviruses

Infections of the central nervous system (CNS) represent a difficult diagnostic problem for both clinicians and microbiologists. In particular, the Herpesviridae family plays a central etiological role in CNS viral infections. These diseases have acquired growing importance in the past few years owing to the increasing number of immunocompromised patients and the availability of new antiviral drugs. Prompt detection and diagnosis of CNS viral infections are critical because most infections are treatable, while a delayed recognition may lead to life-threatening conditions or severe sequelae. The traditional methods for detection of herpesviruses in CNS infections exhibit several drawbacks, whereas the polymerase chain reaction (PCR) on cerebrospinal fluid has revolutionized the neurovirology and is becoming an essential part of the diagnostic work-up of patients with suspected CNS viral infections. A sensitive multiplex PCR method was developed for the simultaneous detection of 6 human herpesviruses (human cytomegalovirus, herpes simplex virus 1, herpes simplex virus 2, Epstein–Barr virus, varicella-zoster virus, and human herpesvirus 6) with the aim of simplifying detection and reducing time and costs. The accuracy, reproducibility, specificity, and sensitivity of these assays were established.

Diagnosis of Herpes Simplex Virus 1 and 2 in Clinical Specimens by Tissue Culture and Polymerase Chain Reaction

2018 ◽  
Vol 27 (3) ◽  
pp. 97-104
Author(s):  
Laila A. Farid ◽  
Eman I. A. Mahmoud ◽  
Walaa S. Khater ◽  
Marwa K. Assaad ◽  
Aly M. Zaki
Keyword(s):  
Polymerase Chain Reaction ◽  
Tissue Culture ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Chain Reaction ◽  
Herpes Simplex Virus 1 ◽  
Clinical Specimens ◽  
Polymerase Chain ◽  
Simplex Virus
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