Transfer of the human genes coding for thymidine kinase and galactokinase to Chinese hamster cells and human-Chinese hamster cell hybrids

1977 ◽  
Vol 3 (3) ◽  
pp. 281-293 ◽  
Author(s):  
G. J. Wullems ◽  
J. van der Horst ◽  
D. Bootsma
Keyword(s):  
Thymidine Kinase ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Chinese Hamster Cells ◽  
Cell Hybrids ◽  
Human Genes

Segregation of recessive phenotypes in somatic cell hybrids: role of mitotic recombination, gene inactivation, and chromosome nondisjunction

1981 ◽  
Vol 1 (4) ◽  
pp. 336-346
Author(s):  
C E Campbell ◽  
R G Worton
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Mitotic Recombination ◽  
Chinese Hamster Cell ◽  
Gene Inactivation ◽  
Chromosome Loss ◽  
Resistance Locus ◽  
Chromosome 2 ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Somatic cell hybrids heterozygous at the emetine resistance locus (emtr/emt+) or the chromate resistance locus (chrr/chr+) are known to segregate the recessive drug resistance phenotype at high frequency. We have examined mechanisms of segregation in Chinese hamster cell hybrids heterozygous at these two loci, both of which map to the long arm of Chinese hamster chromosome 2. To follow the fate of chromosomal arms through the segregation process, our hybrids were also heterozygous at the mtx (methotrexate resistance) locus on the short arm of chromosome 2 and carried cytogenetically marked chromosomes with either a short-arm deletion (2p-) or a long-arm addition (2q+). Karyotype and phenotype analysis of emetine- or chromate-resistant segregants from such hybrids allowed us to distinguish four potential segregation mechanisms: (i) loss of the emt+- or chr+-bearing chromosome; (ii) mitotic recombination between the centromere and the emt or chr loci, giving rise to homozygous resistant segregants; (iii) inactivation of the emt+ or chr+ alleles; and (iv) loss of the emt+- or chr+-bearing chromosome with duplication of the homologous chromosome carrying the emtr or chrr allele. Of 48 independent segregants examined, only 9 (20%) arose by simple chromosome loss. Two segregants (4%) were consistent with a gene inactivation mechanism, but because of their rarity, other mechanisms such as mutation or submicroscopic deletion could not be excluded. Twenty-one segregants (44%) arose by either mitotic recombination or chromosome loss and duplication; the two mechanisms were not distinguishable in that experiment. Finally, in hybrids allowing these two mechanisms to be distinguished, 15 segregants (31%) arose by chromosome loss and duplication, and none arose by mitotic recombination.

scholarly journals A processed gene homologous to the thymidine kinase (TK) gene in Chinese hamster cells

1987 ◽  
Vol 15 (11) ◽  
pp. 4693-4693
Author(s):  
Frederic G. Barr ◽  
Michael W. Lieberman
Keyword(s):  
Thymidine Kinase ◽  
Chinese Hamster ◽  
Chinese Hamster Cells

Correction of Genetic Defects in Mammalian Cells by the Input of Small Amounts of Foreign Genetic Material

1973 ◽  
Vol 13 (3) ◽  
pp. 841-861
Author(s):  
YVONNE L. BOYD ◽  
H. HARRIS
Keyword(s):  
Thymidine Kinase ◽  
Mammalian Cells ◽  
Hybrid Cell ◽  
Genetic Material ◽  
Chinese Hamster ◽  
Heat Sensitivity ◽  
Chinese Hamster Cells ◽  
Inosinic Acid ◽  
Mouse Cells

Chinese hamster cells lacking inosinic acid pyrophosphorylase and mouse cells lacking thymidine kinase were fused with chick erythrocytes. The resultant heterokaryons were cultivated in a selective medium in which possession of these enzymes was essential for cell survival and growth. Clones of cells able to grow in this medium were isolated and studied. A detailed karyological analysis of these clones failed to reveal any chick chromosomes; nor could any chick-specific antigens be detected on the surface of the cells. Nonetheless, clones arising from the fusion of chick erythrocytes with Chinese hamster cells were shown to possess an inosinic acid pyrophosphorylase which had the electrophoretic characteristics of chick inosinic acid pyrophosphorylase. However, the clones arising from the fusion of the chick erythrocytes with the mouse cells had a thymidine kinase with the electrophoretic mobility and heat sensitivity of murine, not chick, thymidine kinase. Both types of hybrid cell have now been cultivated in vitro for 18 months without the loss of thymidine kinase or inosinic acid pyrophosphorylase activity.

Coordinate amplification of metallothionein I and II genes in cadmium-resistant Chinese hamster cells: implications for mechanisms regulating metallothionein gene expression

1985 ◽  
Vol 5 (2) ◽  
pp. 320-329
Author(s):  
B D Crawford ◽  
M D Enger ◽  
B B Griffith ◽  
J K Griffith ◽  
J L Hanners ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Chinese Hamster Cells ◽  
Future Studies ◽  
Noncoding Regions ◽  
Genes Encoding ◽  
Chinese Hamster Cell Line

We describe here the derivation, characterization, and use of clonal cadmium-resistant (Cdr) strains of the Chinese hamster cell line CHO which differ in their metallothionein (MT) induction capacity. By nondenaturing polyacrylamide gel electrophoresis, we showed that the stable Cdr phenotype is correlated with the augmented expression of both isometallothioneins (MTI and MTII). In cells resistant to concentrations of CdCl2 exceeding 20 microM, coordinate amplification of genes encoding both isometallothioneins was demonstrated by using cDNA MT-coding sequence probes and probes specific for 3'-noncoding regions of Chinese hamster MTI and MTII genes. Molecular and in situ hybridization analyses supported close linkage of Chinese hamster MTI and MTII genes, which we have mapped previously to Chinese hamster chromosome 3. This suggests the existence of a functionally related MT gene cluster in this species. Amplified Cdr variants expressing abundant MT and their corresponding Cds parental CHO cells should be useful for future studies directed toward elucidating the mechanisms that regulate expression of the isometallothioneins.

Genetics of Somatic Mammalian Cells: Linkage Studies with Human–Chinese Hamster Cell Hybrids

Nature ◽  
1970 ◽  
Vol 228 (5269) ◽  
pp. 329-332 ◽  
Author(s):  
FA-TEN KÁO ◽  
THEODORE T. PUCK
Keyword(s):  
Mammalian Cells ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Linkage Studies ◽  
Cell Hybrids

Genetic demonstration of mitotic recombination in cultured chinese hamster cell hybrids

Cell ◽  
1984 ◽  
Vol 36 (3) ◽  
pp. 697-707 ◽  
Author(s):  
John J. Wasmuth ◽  
Linda Vock Hall
Keyword(s):  
Chinese Hamster ◽  
Mitotic Recombination ◽  
Chinese Hamster Cell ◽  
Cell Hybrids

scholarly journals c-sis is translocated from chromosome 22 to chromosome 9 in chronic myelocytic leukemia.

1983 ◽  
Vol 158 (1) ◽  
pp. 9-15 ◽  
Author(s):  
J Groffen ◽  
N Heisterkamp ◽  
J R Stephenson ◽  
A G van Kessel ◽  
A de Klein ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Chromosome 9 ◽  
Chromosome 22 ◽  
Chronic Myelocytic Leukemia ◽  
Somatic Cell Hybrids ◽  
Chinese Hamster Cells ◽  
Cell Hybrids ◽  
Myelocytic Leukemia

By analysis of a series of somatic cell hybrids derived by fusion of either mouse or Chinese hamster cells with leukocytes from different chronic myelocytic leukemia (CML) patients or from normal donors, we have localized the human oncogene, c-sis, on the q11 to qter segment of chromosome 22 and demonstrated its translocation from chromosome 22 to chromosome 9 (q34) in CML.

Changes in the cell cycle during culture of mouse-Chinese hamster cell hybrids

1980 ◽  
Vol 102 (2) ◽  
pp. 209-216 ◽  
Author(s):  
Jennifer A. Marshall Graves ◽  
Kenneth W. Koschel
Keyword(s):  
Cell Cycle ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Cell Hybrids
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