scholarly journals A processed gene homologous to the thymidine kinase (TK) gene in Chinese hamster cells

1987 ◽  
Vol 15 (11) ◽  
pp. 4693-4693
Author(s):  
Frederic G. Barr ◽  
Michael W. Lieberman
Keyword(s):  
Thymidine Kinase ◽  
Chinese Hamster ◽  
Chinese Hamster Cells

Correction of Genetic Defects in Mammalian Cells by the Input of Small Amounts of Foreign Genetic Material

1973 ◽  
Vol 13 (3) ◽  
pp. 841-861
Author(s):  
YVONNE L. BOYD ◽  
H. HARRIS
Keyword(s):  
Thymidine Kinase ◽  
Mammalian Cells ◽  
Hybrid Cell ◽  
Genetic Material ◽  
Chinese Hamster ◽  
Heat Sensitivity ◽  
Chinese Hamster Cells ◽  
Inosinic Acid ◽  
Mouse Cells

Chinese hamster cells lacking inosinic acid pyrophosphorylase and mouse cells lacking thymidine kinase were fused with chick erythrocytes. The resultant heterokaryons were cultivated in a selective medium in which possession of these enzymes was essential for cell survival and growth. Clones of cells able to grow in this medium were isolated and studied. A detailed karyological analysis of these clones failed to reveal any chick chromosomes; nor could any chick-specific antigens be detected on the surface of the cells. Nonetheless, clones arising from the fusion of chick erythrocytes with Chinese hamster cells were shown to possess an inosinic acid pyrophosphorylase which had the electrophoretic characteristics of chick inosinic acid pyrophosphorylase. However, the clones arising from the fusion of the chick erythrocytes with the mouse cells had a thymidine kinase with the electrophoretic mobility and heat sensitivity of murine, not chick, thymidine kinase. Both types of hybrid cell have now been cultivated in vitro for 18 months without the loss of thymidine kinase or inosinic acid pyrophosphorylase activity.

scholarly journals Ordered splicing of thymidine kinase pre-mRNA during the S phase of the cell cycle.

1990 ◽  
Vol 10 (10) ◽  
pp. 5591-5595 ◽  
Author(s):  
J M Gudas ◽  
G B Knight ◽  
A B Pardee
Keyword(s):  
Cell Cycle ◽  
Thymidine Kinase ◽  
Chinese Hamster ◽  
S Phase ◽  
3T3 Cells ◽  
Chinese Hamster Cells ◽  
Intervening Sequences ◽  
Intron Removal

Concomitant with the onset of S phase, a series of thymidine kinase (TK) splicing intermediates as well as mature TK mRNA accumulates in the nucleus of BALB/c 3T3 cells. Most of the TK splicing intermediates are retained by oligo(dT)-cellulose chromatography, and, therefore, 3' end formation and polyadenylation probably precede the splicing of TK pre-mRNAs. We have further characterized the TK pre-mRNAs that are present in the nuclei of S-phase cells by using specific probes derived from each of the six TK intervening sequences. Based on the sizes of the pre-mRNAs and their patterns of hybridization with these intron probes, we propose a pathway for intron removal from nascent TK transcripts. Intron excision occurred by a preferred, but not necessarily obligatory, order which appears to have been conserved in mouse and Chinese hamster cells.

Induction of thymidine kinase in enzyme-deficient chinese hamster cells

Cell ◽  
1982 ◽  
Vol 29 (2) ◽  
pp. 483-492 ◽  
Author(s):  
Morgan Harris
Keyword(s):  
Thymidine Kinase ◽  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
Enzyme Deficient

Ordered splicing of thymidine kinase pre-mRNA during the S phase of the cell cycle

1990 ◽  
Vol 10 (10) ◽  
pp. 5591-5595
Author(s):  
J M Gudas ◽  
G B Knight ◽  
A B Pardee
Keyword(s):  
Cell Cycle ◽  
Thymidine Kinase ◽  
Chinese Hamster ◽  
S Phase ◽  
3T3 Cells ◽  
Chinese Hamster Cells ◽  
Intervening Sequences ◽  
Intron Removal

Concomitant with the onset of S phase, a series of thymidine kinase (TK) splicing intermediates as well as mature TK mRNA accumulates in the nucleus of BALB/c 3T3 cells. Most of the TK splicing intermediates are retained by oligo(dT)-cellulose chromatography, and, therefore, 3' end formation and polyadenylation probably precede the splicing of TK pre-mRNAs. We have further characterized the TK pre-mRNAs that are present in the nuclei of S-phase cells by using specific probes derived from each of the six TK intervening sequences. Based on the sizes of the pre-mRNAs and their patterns of hybridization with these intron probes, we propose a pathway for intron removal from nascent TK transcripts. Intron excision occurred by a preferred, but not necessarily obligatory, order which appears to have been conserved in mouse and Chinese hamster cells.

Transfer of the human genes coding for thymidine kinase and galactokinase to Chinese hamster cells and human-Chinese hamster cell hybrids

1977 ◽  
Vol 3 (3) ◽  
pp. 281-293 ◽  
Author(s):  
G. J. Wullems ◽  
J. van der Horst ◽  
D. Bootsma
Keyword(s):  
Thymidine Kinase ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Chinese Hamster Cells ◽  
Cell Hybrids ◽  
Human Genes

Multiplex PCR Analysis of Ultraviolet A and B Induced Delayed and Early Mutations in V79 Chinese Hamster Cells

2004 ◽  
Author(s):  
Jostein Dahle ◽  
Paul Noordhuis ◽  
Trond Stokke ◽  
Debbie Hege Svendsrud ◽  
Egil Kvam
Keyword(s):  
Multiplex Pcr ◽  
Chinese Hamster ◽  
Pcr Analysis ◽  
Ultraviolet A ◽  
Chinese Hamster Cells
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