Propagation of two species of mitochondrial DNA in Chinese hamster-mouse somatic cell hybrids

1983 ◽  
Vol 9 (1) ◽  
pp. 133-139 ◽  
Author(s):  
Laura DeFrancesco
Keyword(s):  
Mitochondrial Dna ◽  
Somatic Cell ◽  
Chinese Hamster ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Mouse Somatic Cell

Retention of both parental mitochondrial DNA species in mouse-Chinese hamster somatic cell hybrids

1984 ◽  
Vol 10 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Steven H. Zuckerman ◽  
Joseph F. Solus ◽  
Frances P. Gillespie ◽  
Jerome M. Eisenstadt
Keyword(s):  
Mitochondrial Dna ◽  
Somatic Cell ◽  
Chinese Hamster ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Transcription and translation of mitochondrial DNA in interspecific somatic cell hybrids

1986 ◽  
Vol 6 (6) ◽  
pp. 1951-1957
Author(s):  
F P Gillespie ◽  
T H Hong ◽  
J M Eisenstadt
Keyword(s):  
Mitochondrial Dna ◽  
Somatic Cell ◽  
Cell Lines ◽  
Hybrid Cell ◽  
Chinese Hamster ◽  
Mitochondrial Rna ◽  
Somatic Cell Hybrids ◽  
Rna Transcripts ◽  
Mitochondrial Rrna ◽  
Cell Hybrids

We examined the mitochondrial transcription and translation products of somatic cell hybrids constructed by the fusion of Chinese hamster and mouse cells. The hybrid cell lines OAC-k, OAC-l, and OAC-m contain approximately equal amounts of hamster and mouse mitochondrial DNA and produced mitochondrial rRNA from both parental species in the same ratio. Cell lines OAC-k, OAC-l, and OAC-m also produced poly(A)+ mouse mitochondrial RNA transcripts comparable in complexity and quantity to poly(A)+ RNA from the mouse parent. However, the overall level of poly(A)+ hamster mitochondrial RNA from these hybrids was significantly reduced compared with that from the hamster parent. The hybrid cells also lacked several poly(A)+ RNA species found in the hamster parent, but contained additional minor transcripts. The mitochondrially coded proteins of the OAC-k, OAC-l, and OAC-m cells were predominantly encoded by the mouse mitochondrial DNA.

Chromosome segregation from cell hybrids. III. Segregation is independent of spindle constitution

Genome ◽  
1987 ◽  
Vol 29 (4) ◽  
pp. 528-531 ◽  
Author(s):  
Paula A. Zelesco ◽  
Jennifer A. Marshall Graves
Keyword(s):  
Somatic Cell ◽  
Key Words ◽  
Chromosome Segregation ◽  
Chinese Hamster ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Mouse Somatic Cell ◽  
Β Tubulin

Hamster β-tubulin (detected as a mutant subunit that confers Colcemid resistance) is either not expressed or is underexpressed in Chinese hamster–mouse somatic cell hybrids. This selectivity of tubulin expression suggests that a uniparental mouse spindle might preferentially engage mouse chromosomes and lead to loss of hamster chromosomes. However, the repression of hamster tubulin was found to have no bearing on the direction of chromosome segregation occurring in eight hybrids studied, some of which segregated predominantly mouse and others hamster chromosomes. Key words: chromosome segregation, cell hybrids, spindle, tubulin.

scholarly journals Transcription and translation of mitochondrial DNA in interspecific somatic cell hybrids.

1986 ◽  
Vol 6 (6) ◽  
pp. 1951-1957 ◽  
Author(s):  
F P Gillespie ◽  
T H Hong ◽  
J M Eisenstadt
Keyword(s):  
Mitochondrial Dna ◽  
Somatic Cell ◽  
Cell Lines ◽  
Hybrid Cell ◽  
Chinese Hamster ◽  
Mitochondrial Rna ◽  
Somatic Cell Hybrids ◽  
Rna Transcripts ◽  
Mitochondrial Rrna ◽  
Cell Hybrids

We examined the mitochondrial transcription and translation products of somatic cell hybrids constructed by the fusion of Chinese hamster and mouse cells. The hybrid cell lines OAC-k, OAC-l, and OAC-m contain approximately equal amounts of hamster and mouse mitochondrial DNA and produced mitochondrial rRNA from both parental species in the same ratio. Cell lines OAC-k, OAC-l, and OAC-m also produced poly(A)+ mouse mitochondrial RNA transcripts comparable in complexity and quantity to poly(A)+ RNA from the mouse parent. However, the overall level of poly(A)+ hamster mitochondrial RNA from these hybrids was significantly reduced compared with that from the hamster parent. The hybrid cells also lacked several poly(A)+ RNA species found in the hamster parent, but contained additional minor transcripts. The mitochondrially coded proteins of the OAC-k, OAC-l, and OAC-m cells were predominantly encoded by the mouse mitochondrial DNA.

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