Assignment of the genes for thymidine kinase and galactokinase toMus musculus chromosome 11 and the preferential segregation of this chromosome in Chinese hamster/mouse somatic cell hybrids

1977 ◽  
Vol 3 (2) ◽  
pp. 121-133 ◽  
Author(s):  
C. A. Kozak ◽  
F. H. Ruddle
Keyword(s):  
Somatic Cell ◽  
Thymidine Kinase ◽  
Chinese Hamster ◽  
Somatic Cell Hybrids ◽  
Chromosome 11 ◽  
Cell Hybrids ◽  
Preferential Segregation ◽  
Mouse Somatic Cell

scholarly journals GENETIC CONTROL OF MITOCHONDRIAL THYMIDINE KINASE IN HUMAN-MOUSE AND MONKEY-MOUSE SOMATIC CELL HYBRIDS

1974 ◽  
Vol 61 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Saul Kit ◽  
Wai-Choi Leung
Keyword(s):  
Somatic Cell ◽  
Thymidine Kinase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Molecular Forms ◽  
Kidney Cells ◽  
Somatic Cell Hybrids ◽  
Human Embryonic Lung ◽  
Cell Hybrids ◽  
Electrophoretic Mobilities ◽  
Mouse Somatic Cell

Distinctive thymidine (dT) kinase molecular forms are present in mouse, human, and monkey mitochondria. Disk polyacrylamide gel electrophoresis (disk PAGE) analyses have shown that the mitochondrial-specific dT kinases differ from cytosol dT kinases in relative electrophoretic mobilities (Rm). Furthermore, the mouse mitochondrial dT kinase differs in Rm value from primate mitochondrial dT kinases. The mouse and primate cytosol dT kinases can also be distinguished. Disk PAGE analyses have been carried out on the cytosol and mitochondrial dT kinases of human-mouse (WIL-8) and monkey-mouse (mK·CVIII) somatic cell hybrids in order to learn whether the mitochondria of the hybrid cells contained murine mitochondrial-specific, primate mitochondrial-specific, or both dT kinases. WIL-8 cells were derived from cytosol dT kinase-negative, mitochondrial dT kinase-positive mouse fibro blasts and from cytosol dT kinase-positive, mitochondrial dT kinase-positive human embryonic lung cells; they contained mostly mouse chromosomes and a few human chromosomes, including the determinant for human cytosol dT kinase. The mK·CVIII cells were derived from cytosol dT kinase-negative, mitochondrial dT kinase-positive mouse kidney cells and from cytosol dT kinase-positive, mitochondrial dT kinase-positive monkey kidney cells; they contained mostly mouse chromosomes and a few monkey chromosomes, including the determinant for monkey cytosol dT kinase. Disk PAGE analyses demonstrated that the mitochondria of human-mouse and monkey-mouse somatic cell hybrids contained the mouse-specific mitochondrial dT kinase but not the human- or monkey-specific mitochondrial dT kinase. These findings suggest that primate cytosol and mitochondrial thymidine kinase genes are coded on different chromosomes.

Chromosome segregation from cell hybrids. III. Segregation is independent of spindle constitution

Genome ◽  
1987 ◽  
Vol 29 (4) ◽  
pp. 528-531 ◽  
Author(s):  
Paula A. Zelesco ◽  
Jennifer A. Marshall Graves
Keyword(s):  
Somatic Cell ◽  
Key Words ◽  
Chromosome Segregation ◽  
Chinese Hamster ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Mouse Somatic Cell ◽  
Β Tubulin

Hamster β-tubulin (detected as a mutant subunit that confers Colcemid resistance) is either not expressed or is underexpressed in Chinese hamster–mouse somatic cell hybrids. This selectivity of tubulin expression suggests that a uniparental mouse spindle might preferentially engage mouse chromosomes and lead to loss of hamster chromosomes. However, the repression of hamster tubulin was found to have no bearing on the direction of chromosome segregation occurring in eight hybrids studied, some of which segregated predominantly mouse and others hamster chromosomes. Key words: chromosome segregation, cell hybrids, spindle, tubulin.

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