Plasma membrane carbohydrate composition and lectin receptors of lymphocytes from Pro-lymphocytic leukaemia

1983 ◽  
Vol 61 (19) ◽  
pp. 941-945 ◽  
Author(s):  
G. H. Farrar ◽  
W. M. Glöckner ◽  
G. Uhlenbruck
Keyword(s):  
Plasma Membrane ◽  
Lymphocytic Leukaemia ◽  
Carbohydrate Composition ◽  
Lectin Receptors

Carbohydrate antigens and lectin receptors of the plasma membrane of carrot cells

PROTOPLASMA ◽  
1989 ◽  
Vol 152 (2-3) ◽  
pp. 123-129 ◽  
Author(s):  
J. P. Knox ◽  
K. Roberts
Keyword(s):  
Plasma Membrane ◽  
Carbohydrate Antigens ◽  
Lectin Receptors ◽  
Carrot Cells

Relationships between anionic sites and lectin receptors in the plasma membrane of Dictyostelium discoideum and their role in phagocytosis

1980 ◽  
Vol 41 (1) ◽  
pp. 89-104
Author(s):  
R. Hellio ◽  
A. Ryter
Keyword(s):  
Plasma Membrane ◽  
Double Labelling ◽  
Con A ◽  
Lectin Receptors ◽  
Latex Beads ◽  
Specific Receptors ◽  
Phagocytic Cups ◽  
The Moment ◽  
Peripheral Cells ◽  
H 30

The disappearance of Wheat Germ Agglutinin (WGA) receptors from the membrane of yeast-engulfing-phagocytic cups in Dictyostelium suggested that these receptors could play a role in yeast adsorption or ingestion. This problem was approached by comparing the fate of WGA, Concanavalin A (Con A) and cationized ferritin (CF) and their effects on the phagocytosis of yeast, bacteria and latex beads. It can be concluded that CF capped in about 30 min and inhibited phagocytosis of any kind of particles for about 15 min. Con A capped in 20–60 min and inhibited phagocytosis of all particles for 1 h 30 min. The time at which phagocytosis started to occur corresponded approximately to the moment at which large areas of plasma membrane were totally devoid of marker. WGA did not cap but induced the formation of large and tight aggregates. The surface of the peripheral cells progressively released WGA in 1 h 30 min. Afterwards, the cells were able to ingest latex beads and bacteria but did not phagocytoze yeast. The latter started to be adsorbed onto the cells and to be ingested only 1 h later. Double labelling experiments showed that CF and Con A receptors were still absent in the plasma membrane, when phagocytosis of any kind of particles started to occur. WGA-labelled cells ingested latex beads and bacteria when their plasma membrane was still devoid of WGA receptors but were able to ingest yeast only after their regeneration. These observations strongly suggest that WGA receptors may correspond to specific receptors for yeast phagocytosis.

scholarly journals Fractionation of membrane proteins on phenylboronic acid-agarose

1982 ◽  
Vol 205 (1) ◽  
pp. 167-171 ◽  
Author(s):  
G T Williams ◽  
A P Johnstone ◽  
P D Dean
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Chronic Lymphocytic Leukaemia ◽  
Lymphocytic Leukaemia ◽  
Phenylboronic Acid ◽  
Immunoglobulin M ◽  
Plasma Membrane Proteins ◽  
Histocompatibility Antigens ◽  
Surface Immunoglobulin ◽  
Hla Dr

Detergent-solubilized plasma-membrane proteins from lymphocytes of patients with chronic lymphocytic leukaemia were applied to phenylboronic acid-agarose. Some of the polypeptides were bound specifically and subsequently eluted with sorbitol. Immunoprecipitation analyses showed that no surface immunoglobulin M was bound, but that most of the histocompatibility antigens HLA-A, HLA-B, HLA-C and HLA-DR were.

Detection of Concannavalin a Receptors in Trophozoites and Cysts of Acanthamoeba sp. (W4) Philippine Isolate

1997 ◽  
Vol 3 (S2) ◽  
pp. 119-120
Author(s):  
A.M. Argayosa ◽  
F.F. Natividad ◽  
R.R. Matias ◽  
G.L. Enriquez
Keyword(s):  
Plasma Membrane ◽  
Cell Adhesion ◽  
Con A ◽  
Strong Fluorescence ◽  
Lectin Receptors ◽  
Lectin Receptor ◽  
Adhesion Sites ◽  
Receptor Sites ◽  
Topographical Distribution ◽  
Green Fluorescent

Distribution of glucose and mannose moieties of Acanthamoeba sp. (W4) Philippine isolate was detected using fluorescem isothiocyanate (FITC)- labeled Concannavalin A (Con A) lectin. Green fluorescent patches around the plasma membrane of agglutinated trophozoites (Fig.1) were observed. Isolated Acanthamoeba cyst exhibited strong fluorescence on the cyst wall Brighter fluorescence was detected on the site of adherence between the Acanthamoeba (W4) cysts and trophozoites (Fig. 1,3). These lectin receptors were concentrated at the uroidal region of the trophozoite. The fluorescence, however, was absent in the newly forming hyaline cap (Fig.4). Upon addition of α -methyl-mannoside (0.5 M), Con A binding to sugar moieties in cyst and trophozoites was blocked and no fluorescence was observed.The binding specificity of Con A-FITC and Acanthamoeba cell surface mannose moieties demonstrate topographical distribution of lectin receptor sites. Ultrastructurally, ferritin-labeled Con A at cell adhesion sites showed clustering of lectin receptors. Occurrence of fluorescence in Naegleria sp. using Con A-FITC has been shown to concentrate at the uroidal region but no fluorescence was seen at the anterior of newly formed pseudopodia.

Lectin receptors on the plasma membrane of soybean cells. Binding and lateral diffusion of lectins

Biochemistry ◽  
1983 ◽  
Vol 22 (16) ◽  
pp. 3969-3975 ◽  
Author(s):  
Thomas N. Metcalf ◽  
John L. Wang ◽  
Karel R. Schubert ◽  
Melvin Schindler
Keyword(s):  
Plasma Membrane ◽  
Lateral Diffusion ◽  
Lectin Receptors

Heterogeneity of Size and Distribution of Intramembrane Particles in the Plasma Membrane of Yeast

1977 ◽  
Vol 35 ◽  
pp. 596-597
Author(s):  
E. Keyhani
Keyword(s):  
Plasma Membrane ◽  
Candida Utilis ◽  
Synthetic Medium ◽  
Freeze Fracture ◽  
Translational Diffusion ◽  
Yeast Cells ◽  
Distilled Water ◽  
Intramembrane Particles ◽  
The Matrix ◽  
Water Cell

The matrix of biological membranes consists of a lipid bilayer into which proteins or protein aggregates are intercalated. Freeze-fracture techni- ques permit these proteins, perhaps in association with lipids, to be visualized in the hydrophobic regions of the membrane. Thus, numerous intramembrane particles (IMP) have been found on the fracture faces of membranes from a wide variety of cells (1-3). A recognized property of IMP is their tendency to form aggregates in response to changes in experi- mental conditions (4,5), perhaps as a result of translational diffusion through the viscous plane of the membrane. The purpose of this communica- tion is to describe the distribution and size of IMP in the plasma membrane of yeast (Candida utilis).Yeast cells (ATCC 8205) were grown in synthetic medium (6), and then harvested after 16 hours of culture, and washed twice in distilled water. Cell pellets were suspended in growth medium supplemented with 30% glycerol and incubated for 30 minutes at 0°C, centrifuged, and prepared for freeze-fracture, as described earlier (2,3).

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