Cloning of DNA libraries from mouse Y chromosomes purified by flow cytometry

1986 ◽  
Vol 12 (3) ◽  
pp. 289-295 ◽  
Author(s):  
Bruno Baron ◽  
Philippe Métézeau ◽  
Didier Hatat ◽  
Christa Roberts ◽  
Michel E. Goldberg ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Y Chromosomes ◽  
Dna Libraries

Identification and Cytogenetic Analysis of an Abnormal Pig Chromosome for Flow Cytometry and Sorting

1993 ◽  
Vol 48 (7-8) ◽  
pp. 645-653 ◽  
Author(s):  
Michael Hausmann ◽  
C. Paul Popescu ◽  
Jeannine Boscher ◽  
Dominique Kerboœf ◽  
Jürgen Dölle ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Marker Chromosome ◽  
Flow Analysis ◽  
Chromosome 1 ◽  
Cell Type ◽  
Metaphase Chromosomes ◽  
Flow Sorting ◽  
Dna Libraries ◽  
Sus Scrofa Domestica

Abstract For cytogenetics of pig (Sus scrofa domestica) and the influence of chromosome aberrations on pig production, high interest exists in flow sorted chromosomes for gene mapping, to estab­lish DNA-libraries, or to produce DNA-probes. Flow karyotyping and sorting as well as slit scan flow analysis of metaphase chromosomes of an abnormal cell type carrying a translocation marker chromosome 6/15 are described. Flow sorting of the largest chromosomes of these cells was performed. After sorting the chromosomes still had a well preserved morphology and were identified microscopically by G-banding. The quality of the band pattern of the sorted chromosomes was compatible to that of isolated chromosomes not subjected to flow cytometry. The sorted fraction showed an enrichment of chromosom e 6/15 and chromosome 1 which have quantitatively about the same integrated fluorescence intensity. Slit scan flow analysis was performed to discriminate these two chromosomes. Metacentric and submetacentric chromosom es were analyzed according to their bimodal slit scan profiles. Profiles of the largest chromosomes were distinguished by their different centromeric indices. Two groups were interpreted as the normal chromosome 1 and the translocation chromosom e 6/15.

Purifying human Y chromosomes by flow cytometry and sorting

Cytometry ◽  
1983 ◽  
Vol 4 (1) ◽  
pp. 88-91 ◽  
Author(s):  
Judith A. Fantes ◽  
Daryll K. Green ◽  
Howard J. Cooke
Keyword(s):  
Flow Cytometry ◽  
Y Chromosomes

Chromosome Sorting by Flow Cytometry: Production of DNA Libraries and Gene Mapping

2003 ◽  
pp. 205-220
Author(s):  
Judith G Fantes ◽  
Daryll K. Green ◽  
Andrew Sharkey
Keyword(s):  
Flow Cytometry ◽  
Gene Mapping ◽  
Chromosome Sorting ◽  
Dna Libraries

Flow cytometry using the monoclonal antibody CD10-Pe/Cy5 is a useful tool to identify follicular lymphoma cells

2001 ◽  
Vol 66 (2) ◽  
pp. 100-106 ◽  
Author(s):  
M. Bellido ◽  
E. Rubiol ◽  
J. Ubeda ◽  
O. Lopez ◽  
C. Estivill ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Flow Cytometry ◽  
Follicular Lymphoma ◽  
Lymphoma Cells

Attack behavior in mice: Implication of the steroid sulfatase gene mapped on the pairing region of the X-Y-chromosomes

2000 ◽  
Author(s):  
P. L. Roubertoux ◽  
S. Mortaud ◽  
L. Nicolas ◽  
I. Le Roy ◽  
S. Tordjma
Keyword(s):  
Attack Behavior ◽  
Steroid Sulfatase ◽  
Y Chromosomes ◽  
Pairing Region

MxA as biomarker for the activity of interferon-alpha in patients with chronic hepatitis: Measurement by flow cytometry

2010 ◽  
Vol 48 (05) ◽  
Author(s):  
W Tillinger ◽  
R Jilch ◽  
P Wunsch ◽  
L Kramer ◽  
S Knapp
Keyword(s):  
Flow Cytometry ◽  
Chronic Hepatitis ◽  
Interferon Alpha
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