Chromosome Sorting by Flow Cytometry: Production of DNA Libraries and Gene Mapping

2003 ◽  
pp. 205-220
Author(s):  
Judith G Fantes ◽  
Daryll K. Green ◽  
Andrew Sharkey
Keyword(s):  
Flow Cytometry ◽  
Gene Mapping ◽  
Chromosome Sorting ◽  
Dna Libraries

Cloning of DNA libraries from mouse Y chromosomes purified by flow cytometry

1986 ◽  
Vol 12 (3) ◽  
pp. 289-295 ◽  
Author(s):  
Bruno Baron ◽  
Philippe Métézeau ◽  
Didier Hatat ◽  
Christa Roberts ◽  
Michel E. Goldberg ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Y Chromosomes ◽  
Dna Libraries

[19] Chromosome sorting by flow cytometry

1987 ◽  
pp. 252-267 ◽  
Author(s):  
Marty Bartholdi ◽  
Julie Meyne ◽  
Kevin Albright ◽  
Mary Luedemann ◽  
Evelyn Campbell ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Chromosome Sorting

scholarly journals Human Gene Mapping and Chromosome Sorting by Laser Technology.

1993 ◽  
Vol 42 (4) ◽  
pp. 212-216
Author(s):  
Nobuyoshi Shimizu ◽  
Shinsei Minoshima ◽  
Jun Kudoh ◽  
Kazuhiko Kawasaki ◽  
Yimin Wang ◽  
...  
Keyword(s):  
Gene Mapping ◽  
Human Gene ◽  
Chromosome Sorting ◽  
Laser Technology ◽  
Human Gene Mapping

Bivariate flow cytometry of farm animal chromosomes: a potential tool for gene mapping

2009 ◽  
Vol 23 (3) ◽  
pp. 203-210 ◽  
Author(s):  
S. C. DIXON ◽  
N. G. A. MILLER ◽  
N. P. CARTER ◽  
E. M. TUCKER
Keyword(s):  
Flow Cytometry ◽  
Gene Mapping ◽  
Farm Animal ◽  
Potential Tool

Identification and Cytogenetic Analysis of an Abnormal Pig Chromosome for Flow Cytometry and Sorting

1993 ◽  
Vol 48 (7-8) ◽  
pp. 645-653 ◽  
Author(s):  
Michael Hausmann ◽  
C. Paul Popescu ◽  
Jeannine Boscher ◽  
Dominique Kerboœf ◽  
Jürgen Dölle ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Marker Chromosome ◽  
Flow Analysis ◽  
Chromosome 1 ◽  
Cell Type ◽  
Metaphase Chromosomes ◽  
Flow Sorting ◽  
Dna Libraries ◽  
Sus Scrofa Domestica

Abstract For cytogenetics of pig (Sus scrofa domestica) and the influence of chromosome aberrations on pig production, high interest exists in flow sorted chromosomes for gene mapping, to estab­lish DNA-libraries, or to produce DNA-probes. Flow karyotyping and sorting as well as slit scan flow analysis of metaphase chromosomes of an abnormal cell type carrying a translocation marker chromosome 6/15 are described. Flow sorting of the largest chromosomes of these cells was performed. After sorting the chromosomes still had a well preserved morphology and were identified microscopically by G-banding. The quality of the band pattern of the sorted chromosomes was compatible to that of isolated chromosomes not subjected to flow cytometry. The sorted fraction showed an enrichment of chromosom e 6/15 and chromosome 1 which have quantitatively about the same integrated fluorescence intensity. Slit scan flow analysis was performed to discriminate these two chromosomes. Metacentric and submetacentric chromosom es were analyzed according to their bimodal slit scan profiles. Profiles of the largest chromosomes were distinguished by their different centromeric indices. Two groups were interpreted as the normal chromosome 1 and the translocation chromosom e 6/15.

Introduction

1978 ◽  
Vol 36 (3) ◽  
pp. 543-544
Author(s):  
W. Bernard
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Nucleic Acid ◽  
Gene Mapping ◽  
Molecular Genetics ◽  
Cell Biology ◽  
Gene Activity ◽  
Close Connection ◽  
Basic Information ◽  
Simple Systems

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.

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