Psychrophilic microorganisms and their cold-active enzymes

1996 ◽  
Vol 17 (5-6) ◽  
pp. 432-437 ◽  
Author(s):  
JE Brenchley
Keyword(s):  
Cold Active Enzymes ◽  
Cold Active

Enzymatic biodegradation of highly p-xylene contaminated soil using cold-active enzymes: A soil column study

2021 ◽  
pp. 127099
Author(s):  
Saba Miri ◽  
Seyyed Mohammadreza Davoodi ◽  
Thomas Robert ◽  
Satinder Kaur Brar ◽  
Richard Martel ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Soil Column ◽  
Column Study ◽  
Cold Active Enzymes ◽  
Cold Active

Biotechnological potential of psychrophilic microorganisms as the source of cold-active enzymes in food processing applications

3 Biotech ◽  
2021 ◽  
Vol 11 (11) ◽  
Author(s):  
Megha Kumari ◽  
Srichandan Padhi ◽  
Swati Sharma ◽  
Loreni Chiring Phukon ◽  
Sudhir P. Singh ◽  
...  
Keyword(s):  
Food Processing ◽  
Biotechnological Potential ◽  
Cold Active Enzymes ◽  
Cold Active

Bacterial diversity and bioprospecting for cold-active enzymes from culturable bacteria associated with sediment from a melt water stream of Midtre Loenbreen glacier, an Arctic glacier

2009 ◽  
Vol 160 (8) ◽  
pp. 538-546 ◽  
Author(s):  
Puram Vishnu Vardhan Reddy ◽  
Singireesu Soma Shiva Nageswara Rao ◽  
Mambatta Shankaranarayanan Pratibha ◽  
Buddhi Sailaja ◽  
Bakka Kavya ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Culturable Bacteria ◽  
Water Stream ◽  
Cold Active Enzymes ◽  
Melt Water ◽  
Cold Active ◽  
Arctic Glacier

scholarly journals EXTRACELLULAR COLD ACTIVE ENDOGLUCANASE AND PIGMENT PRODUCING PSYCHROTOLERANT PENICILLIUM PINOPHILUM

2016 ◽  
Vol 8 (10) ◽  
pp. 164 ◽  
Author(s):  
Abhas Kumar Maharana
Keyword(s):  
Sugarcane Bagasse ◽  
Endoglucanase Activity ◽  
Total Enzyme Activity ◽  
Cold Active Enzymes ◽  
Red Color ◽  
Cold Active ◽  
Penicillium Pinophilum ◽  
Groundnut Shell ◽  
Color Pigment ◽  
Total Enzyme

<p><strong>Objective: </strong>The objective of the present study was on <em>Penicillium pinophilum </em>strain F2 from soil samples of Jammu city having the potentiality to produce alkaline cold active endoglucanase and pigment.</p><p><strong>Methods: </strong><em>Penicillium pinophilum </em>strain F2,<em> </em>a<em> </em>psychrotolerant micro-fungus was isolated from soil of Jammu city, India by taking Czapek’s Dox agar incubated at 15 °C. The strain was screened for production of cold active enzymes by taking various substrates at 15 °C. Final production was done for cold active endoglucanase by using sugarcane bagasse and ground nut shell as substrates. Besides, the strain was also able to produce red color pigment at a low temperature which was further studied to optimize its production by changing pH and growth medium. The produced pigment was used for dyeing of wool and silk, and absorption percentages were also calculated.</p><p><strong>Results: </strong>Screening for the production of cold active enzymes revealed it as a good producer of cellulose followed by lipase and amylase. Endoglucanase production revealed the total enzyme titer (total enzyme activity) was found to be 5.032 folds higher in sugarcane bagasse (38.91 units) than groundnut shell (7.732 units). Endoglucanase activity was maximum 9.82±0.33 units/ml and 2.29±0.31 units/ml after 120 h of incubation at 15 °C by sugarcane bagasse and groundnut shells, respectively. Red color pigment production was maxima at pH 5 in Czapek’s Dox broth. Maximum absorption percentage was seen by the treatment soaked with mordant, i.e. 5% CuSO<sub>4 </sub>(51.52%) and without a mordant, it showed about 45.54%.</p><p><strong>Conclusion: </strong>Due to the above unique features and capability to produce cold active endoglucanase and pigment by strain F2, can be used significantly in various industries.</p>

scholarly journals Aliivibrio wodanis as a production host: development of genetic tools for expression of cold-active enzymes

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Jenny Johansson Söderberg ◽  
Miriam Grgic ◽  
Erik Hjerde ◽  
Peik Haugen
Keyword(s):  
Low Temperature ◽  
Protein Production ◽  
Fluorescent Protein ◽  
Active Form ◽  
Rhodococcus Erythropolis ◽  
Expression Systems ◽  
Cold Adapted ◽  
Genetic Tools ◽  
Cold Active Enzymes ◽  
Cold Active

Abstract Background Heterologous production of cold-adapted proteins currently represents one of the greatest bottlenecks in the ongoing bioprospecting efforts to find new enzymes from low-temperature environments, such as, the polar oceans that represent essentially untapped resources in this respect. In mesophilic expression hosts such as Escherichia coli, cold-adapted enzymes often form inactive aggregates. Therefore it is necessary to develop new low-temperature expression systems, including identification of new host organisms and complementary genetic tools. Psychrophilic bacteria, including Pseudoalteromonas haloplanktis, Shewanella and Rhodococcus erythropolis have all been explored as candidates for such applications. However to date none of these have found widespread use as efficient expression systems, or are commercially available. In the present work we explored the use of the sub-Arctic bacterium Aliivibrio wodanis as a potential host for heterologous expression of cold-active enzymes. Results We tested 12 bacterial strains, as well as available vectors, promoters and reporter systems. We used RNA-sequencing to determine the most highly expressed genes and their intrinsic promoters in A. wodanis. In addition we examined a novel 5′-fusion to stimulate protein production and solubility. Finally we tested production of a set of “difficult-to-produce” enzymes originating from various bacteria and one Archaea. Our results show that cold-adapted enzymes can be produced in soluble and active form, even in cases when protein production failed in E. coli due to the formation of inclusion bodies. Moreover, we identified a 60-bp/20-aa fragment from the 5′-end of the AW0309160_00174 gene that stimulates expression of Green Fluorescent Protein and improves production of cold-active enzymes when used as a 5′-fusion. A 25-aa peptide from the same protein enhanced secretion of a 25-aa-sfGFP fusion. Conclusions Our results indicate the use of A. wodanis and associated genetic tools for low-temperature protein production and indicate that A. wodanis represents an interesting platform for further development of a protein production system that can promote further cold-enzyme discoveries.

Biotechnological Aspects of Cold-Active Enzymes

2017 ◽  
pp. 461-475 ◽  
Author(s):  
Mário Barroca ◽  
Gustavo Santos ◽  
Charles Gerday ◽  
Tony Collins
Keyword(s):  
Cold Active Enzymes ◽  
Cold Active

Cold-active enzymes: Enabling nonthermal processing in food industry

2022 ◽  
pp. 39-53
Author(s):  
Vijayanand Adapa ◽  
L.N. Ramya ◽  
K.K. Pulicherla
Keyword(s):  
Food Industry ◽  
Nonthermal Processing ◽  
Cold Active Enzymes ◽  
Cold Active

Microbial technologies in the production of low-lactose dairy foods / Tecnologías microbiológicas para la elaboración de productos lácteos con bajo contenido en lactosa

1997 ◽  
Vol 3 (3) ◽  
pp. 163-169 ◽  
Author(s):  
G.A. Somkutl ◽  
V.H. Holsinger
Keyword(s):  
Storage Conditions ◽  
Refrigerated Storage ◽  
Milk Products ◽  
Food Grade ◽  
Dairy Foods ◽  
Lactose Maldigestion ◽  
Cold Active Enzymes ◽  
Cold Active ◽  
Alternative Approaches

Low-lactose milk products with 70% or more of the lactose hydrolysed by food grade β-galac tosidase enzymes of yeasts or fungi have become widely accepted for alleviating the symptoms of lactose maldigestion. This condition limits the intake of nutritious dairy foods by large segments of the world's population. Alternative approaches recently proposed for dealing with lactose maldigestion include the supplementation of milk with dormant dairy cultures, treatment of milk with sonicated or permeabilized cultures as food-grade sources of β-galactosidase and the use of cold-active enzymes to hydrolyse lactose in milk under refrigerated storage conditions.

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