Biotechnological potential of psychrophilic microorganisms as the source of cold-active enzymes in food processing applications

3 Biotech ◽  
2021 ◽  
Vol 11 (11) ◽  
Author(s):  
Megha Kumari ◽  
Srichandan Padhi ◽  
Swati Sharma ◽  
Loreni Chiring Phukon ◽  
Sudhir P. Singh ◽  
...  
Keyword(s):  
Food Processing ◽  
Biotechnological Potential ◽  
Cold Active Enzymes ◽  
Cold Active

Cold-Active Enzymes in Food Processing

2018 ◽  
pp. 383-400 ◽  
Author(s):  
Burhan Hamid ◽  
Fayaz A. Mohiddin
Keyword(s):  
Food Processing ◽  
Cold Active Enzymes ◽  
Cold Active

scholarly journals Cold Adaptation Strategies and the Potential of Psychrophilic Enzymes from the Antarctic Yeast, Glaciozyma antarctica PI12

2021 ◽  
Vol 7 (7) ◽  
pp. 528
Author(s):  
Nur Athirah Yusof ◽  
Noor Haza Fazlin Hashim ◽  
Izwan Bharudin
Keyword(s):  
Cold Adaptation ◽  
Multiple Stressors ◽  
Genome Structure ◽  
Adaptation Strategies ◽  
Biotechnological Potential ◽  
Psychrophilic Yeast ◽  
Cold Active Enzymes ◽  
Cold Active ◽  
Glaciozyma Antarctica Pi12 ◽  
And Function

Psychrophilic organisms possess several adaptive strategies which allow them to sustain life at low temperatures between −20 to 20 °C. Studies on Antarctic psychrophiles are interesting due to the multiple stressors that exist on the permanently cold continent. These organisms produce, among other peculiarities, cold-active enzymes which not only have tremendous biotechnological potential but are valuable models for fundamental research into protein structure and function. Recent innovations in omics technologies such as genomics, transcriptomics, proteomics and metabolomics have contributed a remarkable perspective of the molecular basis underpinning the mechanisms of cold adaptation. This review critically discusses similar and different strategies of cold adaptation in the obligate psychrophilic yeast, Glaciozyma antarctica PI12 at the molecular (genome structure, proteins and enzymes, gene expression) and physiological (antifreeze proteins, membrane fluidity, stress-related proteins) levels. Our extensive studies on G. antarctica have revealed significant insights towards the innate capacity of- and the adaptation strategies employed by this psychrophilic yeast for life in the persistent cold. Furthermore, several cold-active enzymes and proteins with biotechnological potential are also discussed.

Cold Active Enzymes in Food Processing

2005 ◽  
Author(s):  
Bo Mattiasson ◽  
H√°kon √ñrn Birgisson ◽  
Rajni Hatti-Kaul
Keyword(s):  
Food Processing ◽  
Cold Active Enzymes ◽  
Cold Active

Enzymatic biodegradation of highly p-xylene contaminated soil using cold-active enzymes: A soil column study

2021 ◽  
pp. 127099
Author(s):  
Saba Miri ◽  
Seyyed Mohammadreza Davoodi ◽  
Thomas Robert ◽  
Satinder Kaur Brar ◽  
Richard Martel ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Soil Column ◽  
Column Study ◽  
Cold Active Enzymes ◽  
Cold Active

A New Alkaliphilic Cold-Active Esterase from the Psychrophilic Marine Bacterium Rhodococcus sp.: Functional and Structural Studies and Biotechnological Potential

2014 ◽  
Vol 172 (6) ◽  
pp. 3054-3068 ◽  
Author(s):  
Concetta De Santi ◽  
Pietro Tedesco ◽  
Luca Ambrosino ◽  
Bjørn Altermark ◽  
Nils-Peder Willassen ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Structural Studies ◽  
Biotechnological Potential ◽  
Cold Active ◽  
Rhodococcus Sp

Bacterial diversity and bioprospecting for cold-active enzymes from culturable bacteria associated with sediment from a melt water stream of Midtre Loenbreen glacier, an Arctic glacier

2009 ◽  
Vol 160 (8) ◽  
pp. 538-546 ◽  
Author(s):  
Puram Vishnu Vardhan Reddy ◽  
Singireesu Soma Shiva Nageswara Rao ◽  
Mambatta Shankaranarayanan Pratibha ◽  
Buddhi Sailaja ◽  
Bakka Kavya ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Culturable Bacteria ◽  
Water Stream ◽  
Cold Active Enzymes ◽  
Melt Water ◽  
Cold Active ◽  
Arctic Glacier

Cold-Active β-Galactosidases: Sources, Biochemical Properties and Their Biotechnological Potential

2016 ◽  
pp. 445-469 ◽  
Author(s):  
Hubert Cieśliński ◽  
Marta Wanarska ◽  
Anna Pawlak-Szukalska ◽  
Ewelina Krajewska ◽  
Monika Wicka ◽  
...  
Keyword(s):  
Biochemical Properties ◽  
Biotechnological Potential ◽  
Cold Active

scholarly journals EXTRACELLULAR COLD ACTIVE ENDOGLUCANASE AND PIGMENT PRODUCING PSYCHROTOLERANT PENICILLIUM PINOPHILUM

2016 ◽  
Vol 8 (10) ◽  
pp. 164 ◽  
Author(s):  
Abhas Kumar Maharana
Keyword(s):  
Sugarcane Bagasse ◽  
Endoglucanase Activity ◽  
Total Enzyme Activity ◽  
Cold Active Enzymes ◽  
Red Color ◽  
Cold Active ◽  
Penicillium Pinophilum ◽  
Groundnut Shell ◽  
Color Pigment ◽  
Total Enzyme

<p><strong>Objective: </strong>The objective of the present study was on <em>Penicillium pinophilum </em>strain F2 from soil samples of Jammu city having the potentiality to produce alkaline cold active endoglucanase and pigment.</p><p><strong>Methods: </strong><em>Penicillium pinophilum </em>strain F2,<em> </em>a<em> </em>psychrotolerant micro-fungus was isolated from soil of Jammu city, India by taking Czapek’s Dox agar incubated at 15 °C. The strain was screened for production of cold active enzymes by taking various substrates at 15 °C. Final production was done for cold active endoglucanase by using sugarcane bagasse and ground nut shell as substrates. Besides, the strain was also able to produce red color pigment at a low temperature which was further studied to optimize its production by changing pH and growth medium. The produced pigment was used for dyeing of wool and silk, and absorption percentages were also calculated.</p><p><strong>Results: </strong>Screening for the production of cold active enzymes revealed it as a good producer of cellulose followed by lipase and amylase. Endoglucanase production revealed the total enzyme titer (total enzyme activity) was found to be 5.032 folds higher in sugarcane bagasse (38.91 units) than groundnut shell (7.732 units). Endoglucanase activity was maximum 9.82±0.33 units/ml and 2.29±0.31 units/ml after 120 h of incubation at 15 °C by sugarcane bagasse and groundnut shells, respectively. Red color pigment production was maxima at pH 5 in Czapek’s Dox broth. Maximum absorption percentage was seen by the treatment soaked with mordant, i.e. 5% CuSO<sub>4 </sub>(51.52%) and without a mordant, it showed about 45.54%.</p><p><strong>Conclusion: </strong>Due to the above unique features and capability to produce cold active endoglucanase and pigment by strain F2, can be used significantly in various industries.</p>

scholarly journals Aliivibrio wodanis as a production host: development of genetic tools for expression of cold-active enzymes

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Jenny Johansson Söderberg ◽  
Miriam Grgic ◽  
Erik Hjerde ◽  
Peik Haugen
Keyword(s):  
Low Temperature ◽  
Protein Production ◽  
Fluorescent Protein ◽  
Active Form ◽  
Rhodococcus Erythropolis ◽  
Expression Systems ◽  
Cold Adapted ◽  
Genetic Tools ◽  
Cold Active Enzymes ◽  
Cold Active

Abstract Background Heterologous production of cold-adapted proteins currently represents one of the greatest bottlenecks in the ongoing bioprospecting efforts to find new enzymes from low-temperature environments, such as, the polar oceans that represent essentially untapped resources in this respect. In mesophilic expression hosts such as Escherichia coli, cold-adapted enzymes often form inactive aggregates. Therefore it is necessary to develop new low-temperature expression systems, including identification of new host organisms and complementary genetic tools. Psychrophilic bacteria, including Pseudoalteromonas haloplanktis, Shewanella and Rhodococcus erythropolis have all been explored as candidates for such applications. However to date none of these have found widespread use as efficient expression systems, or are commercially available. In the present work we explored the use of the sub-Arctic bacterium Aliivibrio wodanis as a potential host for heterologous expression of cold-active enzymes. Results We tested 12 bacterial strains, as well as available vectors, promoters and reporter systems. We used RNA-sequencing to determine the most highly expressed genes and their intrinsic promoters in A. wodanis. In addition we examined a novel 5′-fusion to stimulate protein production and solubility. Finally we tested production of a set of “difficult-to-produce” enzymes originating from various bacteria and one Archaea. Our results show that cold-adapted enzymes can be produced in soluble and active form, even in cases when protein production failed in E. coli due to the formation of inclusion bodies. Moreover, we identified a 60-bp/20-aa fragment from the 5′-end of the AW0309160_00174 gene that stimulates expression of Green Fluorescent Protein and improves production of cold-active enzymes when used as a 5′-fusion. A 25-aa peptide from the same protein enhanced secretion of a 25-aa-sfGFP fusion. Conclusions Our results indicate the use of A. wodanis and associated genetic tools for low-temperature protein production and indicate that A. wodanis represents an interesting platform for further development of a protein production system that can promote further cold-enzyme discoveries.

Sign in / Sign up

Export Citation Format

Share Document