Characterization of the third origin of DNA replication of the genome of insect iridescent virus type 6

Virus Genes ◽  
1992 ◽  
Vol 6 (4) ◽  
pp. 333-342 ◽  
Author(s):  
Kai-Christian Sonntag ◽  
Gholamreza Darai
Keyword(s):  
Dna Replication ◽  
Virus Type ◽  
Iridescent Virus ◽  
The Third ◽  
Origin Of Dna Replication

Characterization of the TRs/IRS origin of DNA replication of herpes simplex virus Type 1

Virology ◽  
1983 ◽  
Vol 130 (2) ◽  
pp. 427-438 ◽  
Author(s):  
Nigel D. Stow ◽  
Elizabeth C. McMonagle
Keyword(s):  
Herpes Simplex Virus ◽  
Dna Replication ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Origin Of Dna Replication ◽  
Simplex Virus

scholarly journals Identification and Characterization of a Second Putative Origin of DNA Replication in a Baculovirus of Orgyia pseudotsugata

Virology ◽  
1995 ◽  
Vol 207 (2) ◽  
pp. 572-576 ◽  
Author(s):  
Christian H. Ahrens ◽  
Margot N. Pearson ◽  
George F. Rohrmann
Keyword(s):  
Dna Replication ◽  
Orgyia Pseudotsugata ◽  
Origin Of Dna Replication ◽  
Putative Origin ◽  
Identification And Characterization

Identification and characterization of the repetitive DNA elementin the genome of insect iridescent virus type 6

Virology ◽  
1988 ◽  
Vol 167 (2) ◽  
pp. 485-496
Author(s):  
M FISCHER ◽  
P SCHNITZLER ◽  
H DELIUS ◽  
G DARAI
Keyword(s):  
Virus Type ◽  
Repetitive Dna ◽  
Iridescent Virus ◽  
Identification And Characterization

Identification and Characterization of a Putative Origin of DNA Replication in the Genome of a Baculovirus Pathogenic for Orgyia pseudotsugata

Virology ◽  
1993 ◽  
Vol 197 (2) ◽  
pp. 715-725 ◽  
Author(s):  
Margot N. Pearson ◽  
Rebecca M. Bjornson ◽  
Christian Ahrens ◽  
George F. Rohrmann
Keyword(s):  
Dna Replication ◽  
Orgyia Pseudotsugata ◽  
Origin Of Dna Replication ◽  
Putative Origin ◽  
Identification And Characterization

Identification and mapping of origins of DNA replication within the DNA sequences of the genome of insect iridescent virus type 6

Virus Genes ◽  
1992 ◽  
Vol 6 (1) ◽  
pp. 19-32 ◽  
Author(s):  
Michaela Handermann ◽  
Paul Schnitzler ◽  
Angela Rösen-Wolff ◽  
Karl Raab ◽  
Kai-Christian Sonntag ◽  
...  
Keyword(s):  
Dna Replication ◽  
Virus Type ◽  
Dna Sequences ◽  
Iridescent Virus

scholarly journals Expression, purification and characterization of the Spodoptera littoralis nucleopolyhedrovirus (SpliNPV) DNA polymerase and interaction with the SpliNPV non-hr origin of DNA replication

2001 ◽  
Vol 82 (7) ◽  
pp. 1767-1776 ◽  
Author(s):  
Jianhe Huang ◽  
David B. Levin
Keyword(s):  
Dna Replication ◽  
Dna Polymerase ◽  
Spodoptera Littoralis ◽  
Expression System ◽  
Functional Characterization ◽  
Eukaryotic Expression ◽  
Replication Assay ◽  
E Coli ◽  
Origin Of Dna Replication

The DNA polymerase from Spodoptera littoralis nucleopolyhedrovirus (SpliNPV) was expressed in, and purified from, prokaryotic and eukaryotic expression systems. While less protein was obtained from the E. coli expression system, SpliNPV DNAPOL purified from E. coli displayed similar biochemical characteristics to DNAPOL expressed in, and subsequently purified from, insect cells (Sf9) using a baculovirus expression system. Biochemical analyses suggested that the DNA polymerase and the 3′–5′ exonuclease activities are intrinsic to the protein. Deletion of the first 80 amino acid residues from the N terminus of the DNAPOL affected neither the DNA polymerase nor the exonuclease activities of the enzyme. Replication products from single-stranded M13 DNA demonstrated that the DNA synthesis activity of SpliNPV DNAPOL is highly processive. Transient expression assays with a set of deletion clones containing the putative SpliNPV non-hr origin of DNA replication permitted functional characterization of sequence elements within the origin fragment. Purified SpliNPV DNAPOL stimulated origin-dependent DNA replication in a cell-free replication assay.

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