scholarly journals Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus

1996 ◽  
Vol 141 (3-4) ◽  
pp. 751-761 ◽  
Author(s):  
H. D. Loemba ◽  
S. Mounir ◽  
H. Mardassi ◽  
D. Archambault ◽  
S. Dea
2013 ◽  
Vol 9 (1) ◽  
pp. 61 ◽  
Author(s):  
Apisit Kittawornrat ◽  
Mark Engle ◽  
Yaowalak Panyasing ◽  
Chris Olsen ◽  
Kent Schwartz ◽  
...  

1997 ◽  
Vol 112 (4) ◽  
pp. 1307-1320 ◽  
Author(s):  
M Geissler ◽  
K Tokushige ◽  
CC Chante ◽  
VR Zurawski ◽  
JR Wands

Shock ◽  
2006 ◽  
Vol 26 (Supplement 1) ◽  
pp. 33
Author(s):  
M. van Griensven ◽  
E. Calzia ◽  
M. Jafarmadar ◽  
N. Rammal ◽  
E. T??gel ◽  
...  

Vaccine ◽  
2014 ◽  
Vol 32 (29) ◽  
pp. 3617-3622 ◽  
Author(s):  
Jung-Ah Lee ◽  
Byungjoon Kwon ◽  
Fernando A. Osorio ◽  
Asit K. Pattnaik ◽  
Nak-Hyung Lee ◽  
...  

2003 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Jaime G. Cajas ◽  
Joyce A. Rojas ◽  
Jorge E. Almansa ◽  
Myriam T. Wilches ◽  
Camilo Sánchez ◽  
...  

<p>Se comparó la respuesta inmune humoral inducida por las proteínas estructurales del virus de la estomatitis vesicular (serotipos Indiana y New Jersey), utilizando sueros de bovinos vacunados e infectados natural y experimentalmente. Para ello, se usó la técnica de inmunotransferencia (<em>Western blot</em>) a través de la cual se observó el reconocimiento exclusivo de la proteína G en sueros de animales vacunados, así como de las proteínas G y N en sueros de animales infectados. Los sueros de los animales infectados experimentalmente presentaron reacción cruzada en el caso de la proteína N. Se realizaron corridos electroforéticos y densitométricos de 13 subclones del virus de la estomatitis vesicular aislados en distintas áreas endémicas del país. Los resultados mostraron una homología completa e dependiente de su lugar de origen, lo cual es coherente con la literatura reportada.</p><p> </p><p><strong>Abstract</strong> </p><p><strong>Comparison between the immune response to vesicular stomatitis proteins virus in vaccinated bovine and  naturally or induced infected ones</strong></p><p>The induced humoral immune response against structural proteins of Indiana and New Jersey serotypes of the vesicular stomatitis virus was compared using serum obtained from vaccinated bovine, natural and experimentally infected ones. It was used Western blot technique to observe the recognition of protein G by serum of vaccinated animals, and the response to proteins G and N by serum of infected animals. Serum of experimentally infected animals showed crossed reaction for protein N.Were carried out electrophoretic and densitometric analysis on 13 vesicular stomatitis virus subclons isolated from various endemic areas of the country.The results showed complete homology, independent from their origin ccording to the reported literature.</p><p> </p><p> </p>


2021 ◽  
Vol 15 (2) ◽  
pp. 4
Author(s):  
Elitha Pulungan

Background: Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the dengue virus (DENV) which spread widely in tropical and subtropical regions of the world. DENV is a single-positive strand RNA virus with a genome size of ± 11kb which encodes three structural proteins, seven non-structural proteins, and two untranslated regions (UTR). The non-structural protein-1 (NS1) of DENV is known to have important role in dengue pathogenesis also promising to be developed as dengue vaccine. Lately, novel vaccine approach by DNA immunization have given new perspective for a safe, stable, and immunogenic vaccine platform. Previously, we have successfully construct DNA vaccine encoding NS1 protein of DENV2 (pUNS1) which express recombinant NS1 protein in-vitro. Thus, in this current study the ability of pUNS1 to induce humoral immune response will be further analyzed by in mice immunization. Methods: Sixteen BALB/c mice aged of 4 weeks were immunized 3 times with 100 µg of pUNS1 or pUMVC4a on 2 week time interval. Blood sampling was carried out just before immunization and termination was done 2 week after last immunization. Titer from individual mice sera against DENV-2 were measure with in-house ELISA. Results: IgG against NS1 protein of DENV2 titer from mice group immunized with recombinant pUNS1 shown high ELISA absorbancies, 5 times higher than pUMVC4a group. This result suggest the ability of pUNS1 to induce humoral immune response against NS1 DENV-2 in-vivo. Conclusion: Recombinant pUNS1 can induce humoral immune response in mice.


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