scholarly journals Plant‐made dengue virus‐like particles produced by co‐expression of structural and non‐structural proteins induce a humoral immune response in mice

2020 ◽  
Author(s):  
Daniel Ponndorf ◽  
Yulia Meshcheriakova ◽  
Eva C. Thuenemann ◽  
Albor Dobon Alonso ◽  
Ross Overman ◽  
...  
Keyword(s):  
Immune Response ◽  
Dengue Virus ◽  
Humoral Immune Response ◽  
Structural Proteins ◽  
Virus Like Particles ◽  
Humoral Immune

scholarly journals Production of dengue virus-like particles serotype-3 in silkworm larvae and their ability to elicit a humoral immune response in mice

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Doddy Irawan Setyo Utomo ◽  
Sabar Pambudi ◽  
Fithriyah Sjatha ◽  
Tatsuya Kato ◽  
Enoch Y. Park
Keyword(s):  
Immune Response ◽  
Dengue Virus ◽  
Humoral Immune Response ◽  
Silkworm Larvae ◽  
Virus Like Particles ◽  
Humoral Immune

scholarly journals Immunogenicity of Recombinant DNA Vaccine Encoding Non-Structural Protein-1 Dengue Virus Serotype-2 in Balb/c Mice

2021 ◽  
Vol 15 (2) ◽  
pp. 4
Author(s):  
Elitha Pulungan
Keyword(s):  
Immune Response ◽  
Dengue Virus ◽  
Dna Vaccine ◽  
Humoral Immune Response ◽  
Structural Proteins ◽  
Ns1 Protein ◽  
Structural Protein ◽  
Humoral Immune ◽  
Virus Serotype ◽  
Positive Strand Rna

Background: Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the dengue virus (DENV) which spread widely in tropical and subtropical regions of the world. DENV is a single-positive strand RNA virus with a genome size of ± 11kb which encodes three structural proteins, seven non-structural proteins, and two untranslated regions (UTR). The non-structural protein-1 (NS1) of DENV is known to have important role in dengue pathogenesis also promising to be developed as dengue vaccine. Lately, novel vaccine approach by DNA immunization have given new perspective for a safe, stable, and immunogenic vaccine platform. Previously, we have successfully construct DNA vaccine encoding NS1 protein of DENV2 (pUNS1) which express recombinant NS1 protein in-vitro. Thus, in this current study the ability of pUNS1 to induce humoral immune response will be further analyzed by in mice immunization. Methods: Sixteen BALB/c mice aged of 4 weeks were immunized 3 times with 100 µg of pUNS1 or pUMVC4a on 2 week time interval. Blood sampling was carried out just before immunization and termination was done 2 week after last immunization. Titer from individual mice sera against DENV-2 were measure with in-house ELISA. Results: IgG against NS1 protein of DENV2 titer from mice group immunized with recombinant pUNS1 shown high ELISA absorbancies, 5 times higher than pUMVC4a group. This result suggest the ability of pUNS1 to induce humoral immune response against NS1 DENV-2 in-vivo. Conclusion: Recombinant pUNS1 can induce humoral immune response in mice.

ANALYSIS OF RECOMBINANT DENGUE VIRUS POLYPEPTIDES FOR DENGUE DIAGNOSIS AND EVALUATION OF THE HUMORAL IMMUNE RESPONSE

2004 ◽  
Vol 71 (2) ◽  
pp. 144-152 ◽  
Author(s):  
FLAVIA BARRETO DOS SANTOS ◽  
EVA HARRIS ◽  
RITA MARIA RIBEIRO NOGUEIRA ◽  
LEE W. RILEY ◽  
MARIZE PEREIRA MIAGOSTOVICH ◽  
...  
Keyword(s):  
Immune Response ◽  
Dengue Virus ◽  
Humoral Immune Response ◽  
Humoral Immune

Humoral Immune Response Against Human Bocavirus VP2 Virus-Like Particles

2008 ◽  
Vol 21 (4) ◽  
pp. 443-450 ◽  
Author(s):  
Juha Lindner ◽  
Lüdya Karalar ◽  
Sandra Zehentmeier ◽  
Annelie Plentz ◽  
Heiko Pfister ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Human Bocavirus ◽  
Virus Like Particles ◽  
Humoral Immune

Cellular and humoral immune response to hepatitis B virus structural proteins in mice after DNA-based immunization

1997 ◽  
Vol 112 (4) ◽  
pp. 1307-1320 ◽  
Author(s):  
M Geissler ◽  
K Tokushige ◽  
CC Chante ◽  
VR Zurawski ◽  
JR Wands
Keyword(s):  
Immune Response ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Humoral Immune Response ◽  
Structural Proteins ◽  
Humoral Immune ◽  
B Virus

scholarly journals Humoral Immune Response in a Mouse Model Induced With Dengue Virus-like Particles Serotypes 1 and 4 Produced in Silkworm Larvae

2021 ◽  
Author(s):  
Doddy Irawan ◽  
Sabar Pambudi ◽  
Enoch Y. Park
Keyword(s):  
Immune Response ◽  
Dengue Virus ◽  
Protein Domain ◽  
Titration Calorimetry ◽  
Heparin Binding ◽  
Global Public Health ◽  
Silkworm Larvae ◽  
Virus Like Particles ◽  
Humoral Immune ◽  
Domain Iii

Abstract Dengue is an arboviral disease, which threatens almost half the global population, and has emerged as the most significant of current global public health challenges. In this study, we prepared dengue virus-like particles (DENV-LPs) consisting of Capsid-Premembrane-Envelope (CprM/E) and Premembrane-Envelope (prM/E) polypeptides from serotype 1 and 4, which were expressed in the silkworms using Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid. 1CprME, 1prME, 4CprME, and 4prME expressed proteins in hemolymph and molecular weight of the purified proteins were 55 kDa, respectively. The purified polypeptides formed spherical Dengue virus-like particles (DENV-LPs) with approximately 30–55 nm in diameter. The immunoelectron microscopy (IEM) images revealed antigens to the surface of a lipid bilayer of DENV-LPs. The heparin-binding assay shows a positive relationship between absorbance and the quantity of E protein domain III (EDIII), which was supported by the isothermal titration calorimetry assay, showing a moderate binding affinity between heparin and DENV-LP. The high correlation between patient sera and DENV-LP reactivities revealed that these DENV-LPs shared similar epitopes with the natural dengue virus. IgG elicitation studies in mice have demonstrated that DENV-LP/CPrMEs elicits a stronger immune response than DENV-LP/prMEs, which lends credence to this claim.

scholarly journals Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus

1996 ◽  
Vol 141 (3-4) ◽  
pp. 751-761 ◽  
Author(s):  
H. D. Loemba ◽  
S. Mounir ◽  
H. Mardassi ◽  
D. Archambault ◽  
S. Dea
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Structural Proteins ◽  
Respiratory Syndrome Virus ◽  
Humoral Immune ◽  
Kinetics Of

scholarly journals Comparación de la respuesta inmunológica a las proteínas del virus de la estomatitis vesicular entre bovinos vacunados e infectados de manera natural o inducida

2003 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Jaime G. Cajas ◽  
Joyce A. Rojas ◽  
Jorge E. Almansa ◽  
Myriam T. Wilches ◽  
Camilo Sánchez ◽  
...  
Keyword(s):  
Immune Response ◽  
New Jersey ◽  
Western Blot ◽  
Vesicular Stomatitis Virus ◽  
Humoral Immune Response ◽  
Structural Proteins ◽  
Protein G ◽  
Humoral Immune ◽  
Blot Technique ◽  
Vesicular Stomatitis

<p>Se comparó la respuesta inmune humoral inducida por las proteínas estructurales del virus de la estomatitis vesicular (serotipos Indiana y New Jersey), utilizando sueros de bovinos vacunados e infectados natural y experimentalmente. Para ello, se usó la técnica de inmunotransferencia (<em>Western blot</em>) a través de la cual se observó el reconocimiento exclusivo de la proteína G en sueros de animales vacunados, así como de las proteínas G y N en sueros de animales infectados. Los sueros de los animales infectados experimentalmente presentaron reacción cruzada en el caso de la proteína N. Se realizaron corridos electroforéticos y densitométricos de 13 subclones del virus de la estomatitis vesicular aislados en distintas áreas endémicas del país. Los resultados mostraron una homología completa e dependiente de su lugar de origen, lo cual es coherente con la literatura reportada.</p><p> </p><p><strong>Abstract</strong> </p><p><strong>Comparison between the immune response to vesicular stomatitis proteins virus in vaccinated bovine and  naturally or induced infected ones</strong></p><p>The induced humoral immune response against structural proteins of Indiana and New Jersey serotypes of the vesicular stomatitis virus was compared using serum obtained from vaccinated bovine, natural and experimentally infected ones. It was used Western blot technique to observe the recognition of protein G by serum of vaccinated animals, and the response to proteins G and N by serum of infected animals. Serum of experimentally infected animals showed crossed reaction for protein N.Were carried out electrophoretic and densitometric analysis on 13 vesicular stomatitis virus subclons isolated from various endemic areas of the country.The results showed complete homology, independent from their origin ccording to the reported literature.</p><p> </p><p> </p>

scholarly journals Impact of Humoral Immune Response and Absorption Effect on Dynamics of Dengue Virus

2017 ◽  
Vol 13 (12) ◽  
pp. 157
Author(s):  
S.D. Perera ◽  
S.S.N. Perera
Keyword(s):  
Immune Response ◽  
Dengue Virus ◽  
Humoral Immune Response ◽  
Dengue Infection ◽  
Endemic Equilibrium ◽  
Absorption Effect ◽  
Globally Asymptotically Stable ◽  
Humoral Immune ◽  
Proposed Model ◽  
Global Threat

Dengue infection represents a global threat causing 50-100 million infections per year and placing half of the world’s population at risk. Even though how infection is controlled and cured rather remains a mystery, antibodies are thought to play a major role in clearing the virus. In this paper, we study the dynamics of dengue virus with humoral immune response and absorption effect. The proposed model incorporates a time delay in production of antibodies. The basic reproduction number R0 is computed and a detailed stability analysis is done. It was found that the model has 3 steady states, namely, infection free equilibrium, no immune equilibrium and the endemic equilibrium. Conditions for R0 were developed for the local stability of these 3 equilibrium states. The global stability was studied using appropriate Lyapunov function and LaSalle’s invariance principle. We then established a condition for which the endemic equilibrium point is globally asymptotically stable. Also it was observed that the virus count goes to negligible levels within 7-14 days after the onset of symptoms.

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