Histological demonstration of immunoreactive metallothionein in sections of human and rat tissue

1985 ◽  
Vol 7 (4) ◽  
pp. 127-130
Author(s):  
Margaret E. Elmes ◽  
Johanna P. Clarkson ◽  
B. Jasani
Keyword(s):  
Rat Tissue

Cloning of the Rat Tissue Factor cDNA and Promoter: Identification of a Serum-response Region

1996 ◽  
Vol 76 (05) ◽  
pp. 697-702 ◽  
Author(s):  
Olivier Taby ◽  
Claire-Lise Rosenfield ◽  
Vladimir Bogdanov ◽  
Yale Nemerson ◽  
Mark B Taubman
Keyword(s):  
Tissue Factor ◽  
General Structure ◽  
Amino Acid Sequences ◽  
Proximal Promoter ◽  
Striking Similarity ◽  
Upstream Sequence ◽  
Tf Gene ◽  
Rat Tissue ◽  
High Degree ◽  
Transcriptional Elements

SummaryTissue factor (TF) initiates coagulation and its expression in vascular smooth muscle cells (VSMC) likely plays a role in the propagation of arterial thrombosis. We report cloning the cDNA and proximal promoter region of the rat TF gene. While maintaining the general structure and organization of the TF molecule, there is a surprising divergence (≈ 18%) between the derived amino acid sequences of the rat and mouse TF. In contrast, there is striking similarity (90%) in the 5’ untranslated regions. High levels of basal promoter activity were seen in rat VSMC with constructs containing 106 bp of sequence downstream from the putative transcription start site and 426 to 103 bp of upstream sequence. Deletion of the sequence from −103 to −79, containing a single SP1 site, removed virtually all of the basal and serum-induced activity. Removal of the NFkB site or two additional upstream SP1 sites had little effect on serum responsiveness. Removal of the 5’ untranslated region abolished most of the basal activity of the TF promoter, suggesting that its high degree of conservation may be due to the presence of transcriptional elements critical for TF expression in rodent VSMC.

Effect of lead acetate on the nitric oxide system in rat tissue

2008 ◽  
Vol 21 (1) ◽  
pp. 175-178
Author(s):  
Isaac Trakhtenberg ◽  
Olena Apykhtina ◽  
Anatoly Kotsuruba ◽  
Julia Korkach ◽  
Irina Andrusishina
Keyword(s):  
Nitric Oxide ◽  
Lead Acetate ◽  
Oxide System ◽  
Rat Tissue ◽  
Nitric Oxide System

scholarly journals THE SEPARATION OF CATHEPSIN FROM MALIGNANT AND NORMAL RAT TISSUE

1939 ◽  
Vol 131 (1) ◽  
pp. 127-134
Author(s):  
Mary E. Maver
Keyword(s):  
Normal Rat ◽  
Rat Tissue

scholarly journals Factors Affecting the Synthesis of Transferrin by Rat Tissue Slices

1969 ◽  
Vol 244 (15) ◽  
pp. 4193-4199
Author(s):  
E H Morgan
Keyword(s):  
Tissue Slices ◽  
Factors Affecting ◽  
Rat Tissue

scholarly journals Variation of Passive Biomechanical Properties of the Small Intestine along Its Length: Microstructure-Based Characterization

2021 ◽  
Vol 8 (3) ◽  
pp. 32
Author(s):  
Dimitrios P. Sokolis
Keyword(s):  
Small Intestine ◽  
Orientation Angle ◽  
Axial Direction ◽  
Material Model ◽  
Biomechanical Properties ◽  
Intestinal Wall ◽  
Model Parameters ◽  
Material Models ◽  
Hyper Elastic ◽  
Rat Tissue

Multiaxial testing of the small intestinal wall is critical for understanding its biomechanical properties and defining material models, but limited data and material models are available. The aim of the present study was to develop a microstructure-based material model for the small intestine and test whether there was a significant variation in the passive biomechanical properties along the length of the organ. Rat tissue was cut into eight segments that underwent inflation/extension testing, and their nonlinearly hyper-elastic and anisotropic response was characterized by a fiber-reinforced model. Extensive parametric analysis showed a non-significant contribution to the model of the isotropic matrix and circumferential-fiber family, leading also to severe over-parameterization. Such issues were not apparent with the reduced neo-Hookean and (axial and diagonal)-fiber family model, that provided equally accurate fitting results. Absence from the model of either the axial or diagonal-fiber families led to ill representations of the force- and pressure-diameter data, respectively. The primary direction of anisotropy, designated by the estimated orientation angle of diagonal-fiber families, was about 35° to the axial direction, corroborating prior microscopic observations of submucosal collagen-fiber orientation. The estimated model parameters varied across and within the duodenum, jejunum, and ileum, corroborating histologically assessed segmental differences in layer thicknesses.

Folate Activity in Rat Tissue Before and After Pteroylglutamic Acid Load.

1963 ◽  
Vol 112 (2) ◽  
pp. 486-489 ◽  
Author(s):  
N. Grossowicz ◽  
M. Rachmilewitz ◽  
G. Izak
Keyword(s):  
Pteroylglutamic Acid ◽  
Before And After ◽  
Acid Load ◽  
Rat Tissue

scholarly journals Immunocytochemical localization of tissue kallikrein in brain ventricular epithelium and hypothalamic cell bodies.

1985 ◽  
Vol 33 (9) ◽  
pp. 951-953 ◽  
Author(s):  
J A Simson ◽  
R Dom ◽  
J Chao ◽  
C Woodley ◽  
L Chao ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Epithelial Cells ◽  
Third Ventricle ◽  
Immunoperoxidase Staining ◽  
Tissue Kallikrein ◽  
Immunocytochemical Localization ◽  
Specific Monoclonal Antibody ◽  
The Third ◽  
Indirect Immunoperoxidase ◽  
Rat Tissue

A specific monoclonal antibody against rat tissue kallikrein was used as the primary antibody for indirect immunoperoxidase staining of rat hypothalamus. Kallikrein was localized in the epithelial cells (ependyma) lining the third ventricle as well as in cell bodies of arcuate, supraoptic, paraventricular, and ventromedial nuclei.

scholarly journals Augmentation of ACTH-induced steroidogenesis in isolated rat adrenal cells by rat tissue extracts and by hormone preparations.

1982 ◽  
Vol 29 (2) ◽  
pp. 251-254 ◽  
Author(s):  
SAYOMI IIDA ◽  
KEIKO MATSUYAMA ◽  
YOSHIHARU ITOH ◽  
KANAME MORIWAKI ◽  
SEIICHIRO TARUI ◽  
...  
Keyword(s):  
Adrenal Cells ◽  
Tissue Extracts ◽  
Rat Tissue ◽  
Isolated Rat

Physical Mapping of the Rat Tissue Kallikrein Family in Two Gene Clusters by Analysis of P1 Bacteriophage Clones

Genomics ◽  
1994 ◽  
Vol 22 (2) ◽  
pp. 404-417 ◽  
Author(s):  
Michelle Southard-Smith ◽  
James C. Pierce ◽  
Raymond J. MacDonald
Keyword(s):  
Physical Mapping ◽  
Gene Clusters ◽  
Tissue Kallikrein ◽  
Rat Tissue
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