Simulated weightlessness and bone metabolism: Decrease of alkaline phosphatase activity in the femoral diaphysis of rats

Abstract. The interaction of vitamin D3 and zinc on bone metabolism was investigated in the femur of weanling rats. Oral administration of vitamin D3 (1.0 μg/100 g body weight) did not cause any increase in the zinc accumulation in the femoral tissue following treatment with zinc sulphate (1.0 mg Zn/100 g). Administration of vitamin D3 or zinc produced significant increases the alkaline phosphatase activity and DNA content of the femoral diaphvsis but not of the epiphysis. The increase in alkaline phosphatase activity was enhanced additionally by simultaneous administration of vitamin D3 and zinc. Moreover, the increase in DNA content was enhanced markedly (about 4 times) by these treatments. At a dose of 0.5 μg of vitamin D3 per 100 g, DNA content was at the control level. This level was increased about 2 times by simultaneous administration of zinc (1.0 mg/100 g). The increase in alkaline phosphatase activity following simultaneous administration of vitamin D3 and zinc was significantly inhibited by treatment with cycloheximide, actinomycin D, or mitomycin C. Also, the increase in DNA content was completely inhibited by mitomycin C treatment. The present data suggest that the combination of vitamin D3 and zinc has a multiple effect on the stimulation of bone growth and mineralization in weanling rats, and that this effect is based on a stimulation of the DNA synthesis in bone cells.

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Effects of Traditional Chinese Medicines on Murine Bone Metabolism in a Microgravity Environment

The American Journal of Chinese Medicine ◽

10.1142/s0192415x03001363 ◽

2003 ◽

Vol 31 (05) ◽

pp. 739-749 ◽

Cited By ~ 6

Author(s):

Qing-Hua Song ◽

Takao Kobayashi ◽

Takayuki Hosoi ◽

Jong-Chol Cyong

Keyword(s):

Alkaline Phosphatase ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Bone Metabolism ◽

Phosphatase Activity ◽

Alkaline Phosphatase Activity ◽

Space Environment ◽

Microgravity Environment ◽

Traditional Chinese Medicines ◽

Chinese Medicines

We investigated the effects of three traditional Chinese medicine prescriptions on changes of bone metabolism in mice, using a gravity device to produce a microgravity environment. We found that Hochu-ekki-to (TJ-41) and Hachimi-jio-gan (TJ-7) suppress the increase in the ratio of serum Ca/P and the increase of calcium in urine. Moreover, TJ-41 and Shin-bu-to (TJ-30) reversed the increase of alkaline phosphatase activity (ALP), and TJ-41 also reversed the decrease of estradiol in the serum. The mechanism may be that the traditional Chinese medicines increased estradiol, causing the decrease of ALP, which induced the changes of Ca and P in serum, leading to a decreased excretion of Ca in urine. In this study, TJ-41 was effective in every parameter while TJ-7 and TJ-30 was effective on some parameters, showing that traditional Chinese medicines have specificities in the space environment. In conclusion, this study suggests that some traditional Chinese medicines may be beneficial for adaptation to a space environment.

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Indicators of bone metabolism in patients of psychiatric clinic on the background of dimephosphone intake

Kazan medical journal ◽

10.17750/kmj2016-669 ◽

2016 ◽

Vol 97 (5) ◽

pp. 669-673

Author(s):

R Yu Il’ina ◽

O O Pasynkova ◽

L R Mukhamedzhanova ◽

L E Ziganshina

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Mental Disorders ◽

Bone Metabolism ◽

Phosphatase Activity ◽

Serum Calcium ◽

Alkaline Phosphatase Activity ◽

Psychiatric Patients ◽

Control Group ◽

Tartrate Resistant Acid Phosphatase

Aim. To study the indicators of bone metabolism in patients of psychiatric clinics on the background of dimephosphone oral intake.Methods. Research was conducted at the Republican Clinical Psychiatric Hospital named after V.M. Bekhterev (Kazan). 64 patients (36 men and 28 women) aged 45 to 68 years (mean age 56.8±6.9 years) with various forms of mental disorders taking psychotropic drugs for the period of 3 years and over were examined. Resorption markers were determined - D-pyridinoline in urine, serum tartrate-resistant acid phosphatase, alkaline phosphatase, and the content of total and ionized serum calcium. All patients were divided into two groups: those who received dimephosphone in the combined systemic therapy and those who did not. The average duration of the dimephosphone administration was 22±4.2 days, the patients undergone on average 2.6±1.44 courses of treatment during 6 months.Results. The activity of tartrate-resistant acid phosphatase in psychiatric patients was higher compared with those of the control group. The maximum value was registered in patients who were not taking dimephosphone. The alkaline phosphatase activity was the highest in mentally healthy patients of the control group. In psychiatric patients treated with dimephosphone, alkaline phosphatase activity was 20% lower compared with the control group. The minimum values of alkaline phosphatase were registered in patients with mental disorders not taking dimephosphone. When studying the concentration of total and ionized serum calcium there were no significant differences between patients of comparison group and psychiatric patients.Conclusion. Shift in processes of bone remodeling toward its resorption indicates a high risk of pathological fractures in psychiatric patients and the need for osteoporosis prevention; identified changes in biochemical markers of osteoporosis on the background of dimephosphone intake allow to recommend it for the treatment of diagnosed osteoporosis in a psychiatric hospital settings.

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LEUCOCYTE METABOLISM IN PREGNANCY

Acta Endocrinologica ◽

10.1530/acta.0.xxxv0575 ◽

1960 ◽

Vol XXXV (IV) ◽

pp. 575-584 ◽

Cited By ~ 2

Author(s):

C. Borel ◽

J. Frei ◽

A. Vannotti

Keyword(s):

Alkaline Phosphatase ◽

Oxygen Consumption ◽

Pregnant Women ◽

Phosphatase Activity ◽

Alkaline Phosphatase Activity ◽

Lactate Production ◽

Glucose Consumption ◽

In Pregnancy

ABSTRACT Enzymatic studies, on leucocytes of pregnant women, show an increase of the alkaline phosphatase activity and a decrease of the glucose consumption and lactate production, as well as of proteolysis. The oxygen consumption, with succinate as substrate, does not vary.

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