Pharmacokinetics of radioiodinated human and ovine growth hormones in transgenic mice expressing bovine growth hormone

1993 ◽  
Vol 2 (4) ◽  
pp. 219-226 ◽  
Author(s):  
D. Turyn ◽  
A. Bartke
Keyword(s):  
Growth Hormone ◽  
Transgenic Mice ◽  
Growth Hormones ◽  
Bovine Growth Hormone

Effects of an S84E Mutation of Bovine Growth Hormone in Transgenic Mice

2006 ◽  
Vol 231 (3) ◽  
pp. 296-302 ◽  
Author(s):  
E. J. H. Schenck ◽  
C. L. Brooks
Keyword(s):  
Growth Hormone ◽  
Acetic Acid ◽  
Transgenic Mice ◽  
Glutamic Acid ◽  
Receptor Binding ◽  
Growth Hormones ◽  
Side Chain ◽  
Bovine Growth Hormone ◽  
Wild Type ◽  
Transgenic Strains

The ability of mutant bovine growth hormones (bGH) to serve as either agonist or antagonist has been demonstrated in transgenic mice. We have prepared two transgenic strains of FVB/N mice, one expressing wild-type bGH and a second with a glutamic acid mutation at serine 84 in helix 2. Comparison of their phenotypes to those of nontransgenic littermates indicates that wild-type bGH induces a previously described phenotype for hyper-somatotrophic mice. In contrast, the replacement of the side chain hydroxyl at serine 84 with acetic acid produced a phenotype that expressed bGH at appreciable concentrations, but failed to elicit the phenotype observed with either an agonist or an antagonist of bGH. These results indicate that serine 84 is crucial for the activity of bGH despite this site being distal to the receptor binding surfaces.

Increased plasma corticosterone levels in bovine growth hormone (bGH) transgenic mice: Effects of ACTH, GH and IGF-I onin vitro adrenal corticosterone production

1996 ◽  
Vol 5 (3) ◽  
pp. 187-192 ◽  
Author(s):  
Marcelo Cecim ◽  
Maria Alvarez-Sanz ◽  
Louis van de Kar ◽  
Sterling Milton ◽  
Andrzej Bartke
Keyword(s):  
Growth Hormone ◽  
Transgenic Mice ◽  
Plasma Corticosterone ◽  
Bovine Growth Hormone ◽  
Igf I ◽  
Adrenal Corticosterone

Echocardiographic evaluation of cardiac function in transgenic mice over-expressing the bovine growth hormone gene

1999 ◽  
Vol 9 ◽  
pp. 156
Author(s):  
M. Bohlooly-Y ◽  
E. Bollano ◽  
J. Törnell ◽  
E. Omerovic ◽  
V. Kujacic ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Transgenic Mice ◽  
Cardiac Function ◽  
Bovine Growth Hormone ◽  
Growth Hormone Gene ◽  
Echocardiographic Evaluation

Ethanol and nicotine consumption and preference in transgenic mice overexpressing the bovine growth hormone gene

1995 ◽  
Vol 50 (4) ◽  
pp. 563-570 ◽  
Author(s):  
Andrzej Bartke ◽  
Jimmy L. Vandergriff ◽  
Robert A. Jensen ◽  
Charles J. Meliska
Keyword(s):  
Growth Hormone ◽  
Transgenic Mice ◽  
Bovine Growth Hormone ◽  
Growth Hormone Gene ◽  
Nicotine Consumption

Effects of bovine growth hormone (GH) expression in transgenic mice on serum and pituitary immunoreactive mouse GH levels and pituitary GH-releasing factor binding sites

1993 ◽  
Vol 129 (5) ◽  
pp. 446-452 ◽  
Author(s):  
AI Sotelo ◽  
A Bartke ◽  
D Turyn
Keyword(s):  
Growth Hormone ◽  
Transgenic Mice ◽  
Binding Sites ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Serum Levels ◽  
Bovine Growth Hormone ◽  
Complete Suppression ◽  
Over Expression ◽  
Pituitary Glands ◽  
Scatchard Plots

Pituitary and serum levels of homologous growth hormone (GH) and characteristics of specific GH-releasing factor (GHRF) binding to pituitary homogenates were examined in transgenic mice expressing bovine GH (bGH) gene regulated by different promoters [mouse metallothionein-I (MT) or phosphoenolpyruvate carboxykinase (PEPCK)] and in their normal littermates. Pituitary GH concentration and GHRF binding were reduced by approximately 50% in transgenic MT-bGH mice in which serum bGH levels were about 20 μg/l and by approximately 95% in transgenic PEPCK-bGH mice in which serum bGH levels were tenfold higher. Suppression of plasma immunoreactive mouse GH (mGH) levels was detected in MT-bGH but not in PEPCK-bGH animals, presumably due to cross-reaction of the antiserum employed with bGH. Scatchard plots of GHRF binding to washed homogenates of pituitary glands from normal and young adult MT-bGH transgenic mice were curvilinear, indicating the presence of two types of binding sites, with low and high affinities. Both types of binding sites were reduced in number in MT-bGH transgenic mice without changes in their affinity. In 5–7-month-old MT-bGH transgenic mice there were changes in pituitary GH levels, in GHRF binding levels and in characteristics of GHRF binding that closely resembled the alterations described previously in aging rats. We conclude that over-expression of heterologous GH genes in transgenic mice can lead to partial or virtually complete suppression of somatotroph function, depending on the levels of heterologous GH in the circulation, and that transgenic MT-bGH mice exhibit symptoms of remarkably early onset of neuroendocrine aging.

Regulation of immunity in rats by lactogenic and growth hormones

1983 ◽  
Vol 102 (3) ◽  
pp. 351-357 ◽  
Author(s):  
Eva Nagy ◽  
Istvan Berczi ◽  
Henry G. Friesen
Keyword(s):  
Growth Hormone ◽  
Contact Dermatitis ◽  
Blood Cells ◽  
Antibody Formation ◽  
Human Growth ◽  
Growth Hormones ◽  
Placental Lactogen ◽  
Bovine Growth Hormone ◽  
Human Placental Lactogen ◽  
Rat Growth

Abstract. Antibody formation to sheep red blood cells and the development of contact dermatitis in response to dinitrochlorobenzene are impaired in hypophysectomized (Hypo-X) rats. Rat prolactin, rat growth hormone, bovine prolactin, bovine growth hormone, human placental lactogen and human growth hormone all restored the immunological competence of Hypo-X animals. The possible mechanism of action of these hormones on immune reactions is discussed.

scholarly journals Conversion of bovine growth hormone cysteine residues to serine affects secretion by cultured cells and growth rates in transgenic mice.

1992 ◽  
Vol 6 (4) ◽  
pp. 598-606
Author(s):  
X Z Chen ◽  
A W Shafer ◽  
J S Yun ◽  
Y S Li ◽  
T E Wagner ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Transgenic Mice ◽  
Growth Rates ◽  
Cultured Cells ◽  
Cysteine Residues ◽  
Bovine Growth Hormone

scholarly journals Myocardial hypertrophy in transgenic mice overexpressing the bovine growth hormone (bGH) gene

2000 ◽  
Vol 247 (5) ◽  
pp. 546-552 ◽  
Author(s):  
M. L. X. Fu ◽  
J. Tornell ◽  
W. Schulze ◽  
J. Hoebeke ◽  
O. G. P. Isaksson ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Transgenic Mice ◽  
Myocardial Hypertrophy ◽  
Bovine Growth Hormone
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