In vitro effects of hyaluronan on prostaglandin E2 induction by interleukin-1 in rabbit articular chondrocytes

1993 ◽  
Vol 38 (S1) ◽  
pp. 122-125 ◽  
Author(s):  
M. Akatsuka ◽  
Y. Yamamoto ◽  
K. Tobetto ◽  
T. Yasui ◽  
T. Ando
Keyword(s):  
Prostaglandin E2 ◽  
Articular Chondrocytes ◽  
Interleukin 1 ◽  
In Vitro Effects ◽  
Rabbit Articular Chondrocytes

scholarly journals Interleukin-6 (IL-6) is an intermediate in IL-1-induced proliferation of leukemic human megakaryoblasts

Blood ◽  
1990 ◽  
Vol 76 (10) ◽  
pp. 1972-1979 ◽  
Author(s):  
MA Brach ◽  
B Lowenberg ◽  
L Mantovani ◽  
U Schwulera ◽  
R Mertelsmann ◽  
...  
Keyword(s):  
Thymidine Incorporation ◽  
Interleukin 1 ◽  
Leukemic Cells ◽  
Megakaryoblastic Leukemia ◽  
Gm Csf ◽  
Endogenous Production ◽  
Neutralizing Monoclonal Antibody ◽  
In Vitro Effects ◽  
Stimulating Factor

Abstract We have examined the in vitro effects of recombinant human (rh) interleukin-1 (IL-1) on the growth of purified megakaryoblasts obtained from patients with acute megakaryoblastic leukemia. We demonstrate that both IL-1 alpha and IL-1 beta treatment of these cells led to stimulation of DNA synthesis (as shown by increase of 3H-thymidine incorporation up to 35-fold) and also resulted in colony formation of leukemic megakaryoblasts. However, the stimulatory effect of IL-1 was dependent on endogenous production of IL-6, because addition of neutralizing monoclonal antibody (MoAb) to IL-6 abrogated the stimulatory activity of IL-1. In contrast, neutralizing MoAbs to granulocyte (G)-colony stimulating factor (CSF), granulocyte-macrophage (GM)-CSF, and macrophage (M)-CSF failed to counteract the growth- enhancing effects of IL-1. Leukemic megakaryoblasts accumulated IL-6 mRNA and released IL-6 protein into their culture supernatant when exposed to rh IL-1 but failed to disclose transcripts for G-, GM-, and M-CSF under these conditions. Analysis of IL-6 receptor (IL-6R) transcript levels demonstrated that megakaryoblasts constitutively expressed IL-6R mRNA and that these transcripts are down-regulated to undetectable levels upon exposure to IL-1 and IL-6. Increase of 3H- thymidine incorporation by megakaryoblasts could be duplicated by exogenous IL-6 that could be blocked by neutralizing MoAb to IL-6. In conclusion, our results suggest that leukemic megakaryoblasts could produce and secrete IL-6, and express IL-6R, and that the growth- enhancing effect of IL-1 on these cells is indirect, via production of IL-6 by leukemic cells.

Inhibition of interleukin-1 (IL-1) induced neutral proteases from rabbit articular chondrocytes by WY-46,135 and WY-48,989

1991 ◽  
Vol 34 (1-2) ◽  
pp. 223-225 ◽  
Author(s):  
R. G. Caccese ◽  
J. F. DiJoseph ◽  
J. S. Skotnicki ◽  
L. E. Borella ◽  
L. M. Adams
Keyword(s):  
Articular Chondrocytes ◽  
Interleukin 1 ◽  
Neutral Proteases ◽  
Rabbit Articular Chondrocytes

scholarly journals Induction of Osteogenic Protein-1 Expression by Interleukin-1.BETA. in Cultured Rabbit Articular Chondrocytes.

2002 ◽  
Vol 197 (2) ◽  
pp. 101-109 ◽  
Author(s):  
SATOSHI YOSHIDA ◽  
YOSHIKO KUBOTA ◽  
TAMOTSU TOBA ◽  
SABURO HORIUCHI ◽  
TADASHI SHIMAMURA
Keyword(s):  
Articular Chondrocytes ◽  
Interleukin 1 ◽  
Interleukin 1 Beta ◽  
Osteogenic Protein ◽  
Rabbit Articular Chondrocytes

In vitro effects of prostaglandin E2 on leucocytes from sticklebacks (Gasterosteus aculeatus) infected and not infected with the cestode Schistocephalus solidus

2014 ◽  
Vol 41 (2) ◽  
pp. 473-481 ◽  
Author(s):  
Ivan A. Kutyrev ◽  
Frederik Franke ◽  
Janine Büscher ◽  
Joachim Kurtz ◽  
Jörn P. Scharsack
Keyword(s):  
Prostaglandin E2 ◽  
Gasterosteus Aculeatus ◽  
Schistocephalus Solidus ◽  
In Vitro Effects

In vitro effects of meloxicam on metabolism in articular chondrocytes from dogs with naturally occurring osteoarthritis

2013 ◽  
Vol 74 (9) ◽  
pp. 1198-1205 ◽  
Author(s):  
Steven C. Budsberg ◽  
Aaron M. Stoker ◽  
Spencer A. Johnston ◽  
William Liska ◽  
Lisa R. Reno ◽  
...  
Keyword(s):  
Articular Chondrocytes ◽  
Naturally Occurring ◽  
In Vitro Effects
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