Biological effects of low intensity Helium-Neon and Gallium-Arsenide laser irradiation on embryonic chick heart fragments in vitro

1989 ◽  
Vol 4 (2) ◽  
pp. 97-102 ◽  
Author(s):  
Leo De Ridder ◽  
Marleen Verbeke ◽  
Hubert Thierens
Keyword(s):  
Gallium Arsenide ◽  
Laser Irradiation ◽  
Biological Effects ◽  
Embryonic Chick ◽  
Low Intensity ◽  
Chick Heart ◽  
Embryonic Chick Heart ◽  
Helium Neon

Apamin, a highly potent fetal L-type Ca2+ current blocker in single heart cells

1992 ◽  
Vol 262 (2) ◽  
pp. H463-H471 ◽  
Author(s):  
G. Bkaily ◽  
A. Sculptoreanu ◽  
D. Jacques ◽  
D. Economos ◽  
D. Menard
Keyword(s):  
Cell Voltage ◽  
Slow Inward Current ◽  
Heart Cell ◽  
Heart Cells ◽  
Dependent Manner ◽  
Embryonic Chick ◽  
Ventricular Cells ◽  
Chick Heart ◽  
Embryonic Chick Heart

Apamin, a bee venom polypeptide, was reported to block the naturally occurring Ca2+ slow action potentials (APs) in cultured cell reaggregates from old chick hearts [Bkaily, G. et al. Am. J. Physiol. 248 (Heart Circ. Physiol. 17): H961-H965, 1985] as well as the tetrodotoxin (TTX)- and Mn(2+)-insensitive slow Na+ current in young embryonic chick heart cells (Bkaily, G. In Vitro Toxicology. Academic, In press; Bkaily et al. J. Mol. Cell. Cardiol. 23: 25-39, 1991). With the use of the whole cell voltage-clamp technique in single ventricular cells from 10-day-old chick embryos and 17- to 20-wk-old human fetuses, two types of Ca2+ currents (ICa), T and L, were found. These two types of slow inward current in both heart preparations were nearly similar in their voltage, kinetics, and pharmacology. Apamin, a slow Ca2+ action potential blocker in old embryonic chick heart, was found to block the L-type ICa (IL) in a dose-dependent manner without affecting the T-type ICa in both heart cell preparations. The blockade of the IL by apamin was completely reversible upon washout with apamin-free solution. Therefore, when compared with nifedipine or to PN 200-110, apamin seems to be a highly potent L-type Ca2+ channel blocker in heart cells.

Acetylcholinesterase-acetylcholine, an enzyme system essential to rhythmicity in the preneural embryonic chick heart

1966 ◽  
Vol 154 (3) ◽  
pp. 675-683 ◽  
Author(s):  
George Hugo Paff ◽  
Robert Joseph Boucek ◽  
Thorne Parsons Glander
Keyword(s):  
Enzyme System ◽  
Embryonic Chick ◽  
Chick Heart ◽  
Embryonic Chick Heart
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