Hepatitis A-virus in cell culture: I. Propagation of different hepatitis A-virus isolates in a fetal rhesus monkey kidney cell line (Frhk-4)

1980 ◽  
Vol 168 (4) ◽  
pp. 239-248 ◽  
Author(s):  
Bertram Flehmig
Keyword(s):  
Cell Culture ◽  
Rhesus Monkey ◽  
Cell Line ◽  
Kidney Cell ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Kidney Cell Line ◽  
Monkey Kidney Cell ◽  
Fetal Rhesus Monkey Kidney ◽  
Virus Isolates

Inactivation of Hepatitis a Virus by Heat and Formaldehyde

1985 ◽  
Vol 17 (10) ◽  
pp. 43-45 ◽  
Author(s):  
B. Flehmig ◽  
A. Billing ◽  
A. Vallbracht ◽  
K. Botzenhart
Keyword(s):  
Rhesus Monkey ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Heat Inactivation ◽  
Kidney Cell Line ◽  
Monkey Kidney Cell ◽  
Complete Inactivation ◽  
Formaldehyde Treatment ◽  
Different Temperatures ◽  
Fetal Rhesus Monkey Kidney

The GBM strain of hepatitis A virus adapted to a fetal rhesus monkey kidney cell line (Frhk-4/R) was treated and inactivated at several different temperatures and with several different formaldehyde concentrations. Heat inactivation showed that no inactivation occurred after treatment for 60 minutes at 37°C. Treatment for 60 minutes at 50°C reduced infectivity by 1 log 10 step ; treatment for 60 minutes at 60°C, by 4 log 10 steps. Treatment for 30 minutes at 70°C resulted in complete inactivation of HAV. Complete inactivation by formaldehyde treatment was achieved only with a 0.35% concentration. Treatment with lower concentrations resulted in incomplete HAV inactivation.

Identification of an exogenous retrovirus (foamy virus type 1) in Rhesus monkey kidney cell culture: significance to viral diagnostics

1998 ◽  
Vol 11 (2) ◽  
pp. 149-153 ◽  
Author(s):  
S.M. Lipson ◽  
M.E. Match ◽  
D.H. Shepp ◽  
M.S. Lotlikar ◽  
S. Teichberg ◽  
...  
Keyword(s):  
Cell Culture ◽  
Rhesus Monkey ◽  
Virus Type ◽  
Kidney Cell ◽  
Foamy Virus ◽  
Monkey Kidney ◽  
Monkey Kidney Cell ◽  
Kidney Cell Culture ◽  
Viral Diagnostics
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