Effects of TSH and cyclic AMP on the human thyroid cells cultured in a chemically defined medium

1977 ◽  
Vol 33 (2) ◽  
pp. 281-282 ◽  
Author(s):  
Y. Kaneko
Keyword(s):  
Cyclic Amp ◽  
Defined Medium ◽  
Human Thyroid ◽  
Chemically Defined Medium ◽  
Thyroid Cells ◽  
Cells Cultured

CHANGES IN MEMBRANE POTENTIAL OF CULTURED PORCINE AND HUMAN THYROID CELLS IN RESPONSE TO THYROTROPHIN AND OTHER AGENTS

1981 ◽  
Vol 88 (2) ◽  
pp. 187-NP ◽  
Author(s):  
J. R. BOURKE ◽  
K. L. CARSELDINE ◽  
S. H. FERRIS ◽  
G. J. HUXHAM ◽  
S. W. MANLEY
Keyword(s):  
Membrane Potential ◽  
Cyclic Amp ◽  
Human Thyroid ◽  
Resting Membrane Potential ◽  
Prostaglandin E ◽  
Follicular Cells ◽  
Thyroid Cells ◽  
Enhanced Sensitivity ◽  
Long Acting ◽  
Cells Cultured

Thyrotrophin (TSH), cyclic AMP, cyclic GMP and 1-methyl-3-isobutyl-xanthine (MIX) promoted the reassociation of isolated porcine and human thyroid cells into follicular structures in culture and stimulated the uptake of radio-iodide. Monolayer cells were present in all cultures, but in decreasing proportions as the concentration of stimulator was increased. The resting membrane potential of porcine thyroid cells cultured for 4 days in the presence of TSH was −54 ± 3·6 (mean ± s.d.) mV for follicular cells and −31 ± 2·6 mV for monolayer cells. In the absence of TSH, only monolayer cells were present and their membrane potential was −24 ± 2·0 mV. Removal of hormone by washing resulted in hyperpolarization to −70 ± 2·9 mV (follicular cells) or −59 ± 3·4 mV (monolayer cells). Subsequent replacement of TSH, or addition of cyclic AMP, MIX, prostaglandin E1 (PGE1) or long-acting thyroid stimulator immunoglobulin resulted in depolarization of previously hyperpolarized cells, to approximately the membrane potential observed before washing. Incubation in MIX resulted in enhanced sensitivity to the depolarizing effect of TSH. Cells cultured in the absence of TSH were unresponsive to TSH or other stimulators. The membrane potential of human thyroid cells behaved similarly in response to TSH, to hormone removal and replacement, and to MIX and PGE1.

Stimulation by thyrotropin and cyclic AMP of the proliferation of quiescent canine thyroid cells cultured in a defined medium containing insulin

FEBS Letters ◽  
1983 ◽  
Vol 157 (2) ◽  
pp. 323-329 ◽  
Author(s):  
Pierre P. Roger ◽  
Patricia Servais ◽  
Jacques E. Dumont
Keyword(s):  
Cyclic Amp ◽  
Defined Medium ◽  
Thyroid Cells ◽  
Cells Cultured

Thyrotrophin regulation of apical and basal exocytosis of thyroglobulin by porcine thyroid monolayers

1990 ◽  
Vol 4 (3) ◽  
pp. 193-199 ◽  
Author(s):  
M. Chambard ◽  
D. Depetris ◽  
D. Gruffat ◽  
S. Gonzalvez ◽  
J. Mauchamp ◽  
...  
Keyword(s):  
Cyclic Amp ◽  
Culture System ◽  
Basal Medium ◽  
Pleiotropic Effect ◽  
Thyroid Cells ◽  
Chase Period ◽  
Thyroglobulin Gene ◽  
Bovine Tsh ◽  
Tsh Stimulation ◽  
Cells Cultured

ABSTRACT Exocytosis, the ultimate step in thyroglobulin secretion, has been studied in porcine thyroid cells cultured in monolayers on the permeable bottom of culture chambers. We have previously demonstrated, using this culture system, that apical secretion accounts for 85–95% of total secretion of newly synthesized thyroglobulin. When cells were cultured for several days with bovine TSH (25 μU/ml) in the basal medium, the rate of glycoprotein accumulation in the upper compartment was three times higher than that in the absence of TSH. In contrast, the rate of thyroglobulin released into the basal medium (5–15% of total secreted thyroglobulin) appeared unmodified by chronic TSH stimulation. To investigate the effect of acute TSH stimulation on thyroglobulin exocytosis in the apical and basal compartments, pulse-chase experiments were carried out with the same culture system. The release of radiolabelled thyroglobulin (1·5-h pulse) into the apical medium was increased threefold during the 2-h chase period under TSH stimulation. The radiolabelled thyroglobulin released into the basal medium was increased only 1·5- to 2-fold, and stimulation disappeared after 1 h. The effect of TSH was maximal when the chase medium contained 50 μU TSH/ml. However, cells cultured for several days in the presence of 25 μU TSH/ml before the pulse-chase experiment, appeared desensitized to acute TSH stimulation. Similar responses were observed when the chase medium contained 8-chloro-cyclic AMP or cholera toxin. This study provides another example of the pleiotropic effect of TSH, mediated by cyclic AMP, on the sequential steps of thyroglobulin gene expression in cultured thyroid cells in which the polar character of the epithelial cells is well preserved.

Developmental features of rat cerebellar neural cells cultured in a chemically defined medium

1986 ◽  
Vol 15 (3) ◽  
pp. 289-301 ◽  
Author(s):  
V. Gallo ◽  
M. T. Ciotti ◽  
F. Aloisi ◽  
G. Levi
Keyword(s):  
Defined Medium ◽  
Neural Cells ◽  
Chemically Defined Medium ◽  
Developmental Features ◽  
Cells Cultured

FSH induction of functional LH receptors in granulosa cells cultured in a chemically defined medium

Nature ◽  
1979 ◽  
Vol 279 (5711) ◽  
pp. 336-338 ◽  
Author(s):  
G. F. ERICKSON ◽  
C. WANG ◽  
A. J. W. HSUEH
Keyword(s):  
Granulosa Cells ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Cells Cultured
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