High-frequency transformation ofStaphylococcus aureus by electroporation

1990 ◽  
Vol 21 (6) ◽  
pp. 373-376 ◽  
Author(s):  
Ginger Rhoads Kraemer ◽  
John J. Iandolo
Keyword(s):  
High Frequency ◽  
Ofstaphylococcus Aureus ◽  
Frequency Transformation ◽  
High Frequency Transformation

Analysis of a high frequency transformation system for Ophiostoma ulmi, the causal agent of Dutch elm disease

1991 ◽  
Vol 225 (1) ◽  
pp. 168-176 ◽  
Author(s):  
J. C. Royer ◽  
K. Dewar ◽  
M. Hubbes ◽  
P. A. Horgen
Keyword(s):  
High Frequency ◽  
Causal Agent ◽  
Dutch Elm Disease ◽  
Transformation System ◽  
Ophiostoma Ulmi ◽  
Frequency Transformation ◽  
High Frequency Transformation

scholarly journals High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

1979 ◽  
Vol 76 (3) ◽  
pp. 1035-1039 ◽  
Author(s):  
K. Struhl ◽  
D. T. Stinchcomb ◽  
S. Scherer ◽  
R. W. Davis
Keyword(s):  
High Frequency ◽  
Dna Molecules ◽  
Frequency Transformation ◽  
Autonomous Replication ◽  
High Frequency Transformation ◽  
Hybrid Dna

High frequency transformation of Zymomonas mobilis by plasmid DNA

1987 ◽  
Vol 6 (4) ◽  
pp. 247-258 ◽  
Author(s):  
Ping Su ◽  
Amanda E Goodman
Keyword(s):  
Plasmid Dna ◽  
High Frequency ◽  
Zymomonas Mobilis ◽  
Frequency Transformation ◽  
High Frequency Transformation

scholarly journals Mutational analysis of the consensus sequence of a replication origin from yeast chromosome III.

1990 ◽  
Vol 10 (8) ◽  
pp. 3917-3925 ◽  
Author(s):  
J V Van Houten ◽  
C S Newlon
Keyword(s):  
Base Pair ◽  
High Frequency ◽  
Mutational Analysis ◽  
Plasmid Stability ◽  
Consensus Sequence ◽  
Point Mutations ◽  
Autonomously Replicating Sequence ◽  
Frequency Transformation ◽  
The Core ◽  
High Frequency Transformation

Yeast autonomously replicating sequence (ARS) elements contain an 11-base-pair core consensus sequence (5'-[A/T]TTTAT[A/G]TTT[A/T]-3') that is required for function. The contribution of each position within this sequence to ARS activity was tested by creating all possible single-base mutations within the core consensus sequence of ARS307 (formerly called the C2G1 ARS) and testing their effects on high-frequency transformation and on plasmid stability. Of the 33 mutations, 22 abolished ARS function as measured by high-frequency transformation, 7 caused more than twofold reductions in plasmid stability, and 4 had no effect on plasmid stability. Mutations that reduced or abolished ARS activity occurred at each position in the consensus sequence, demonstrating that each position of this sequence contributes to ARS function. Of the four mutations that had no effect on ARS activity, three created alternative perfect matches to the core consensus sequence, demonstrating that the alternate bases allowed by the consensus sequence are, indeed, interchangeable. In addition, a change from T to C at position 6 did not perturb wild-type efficiency. To test whether the essential region extends beyond the 11-base-pair consensus sequence, the effects on plasmid stability of point mutations one base 3' to the T-rich strand of the core consensus sequence (position 12) and deletion mutations that altered bases 5' to the T-rich strand of the core consensus sequence were examined. An A at position 12 or the removal of three T residues 5' to the core consensus sequence severely diminished ARS efficiency, showing that the region required for full ARS efficiency extends beyond the core consensus sequence in both directions.

scholarly journals High frequency transformation of Mucor with recombinant plasmid DNA

1984 ◽  
Vol 49 (7) ◽  
pp. 691-702 ◽  
Author(s):  
Robyn van Heeswijck ◽  
M. Isabel G. Roncero
Keyword(s):  
Plasmid Dna ◽  
High Frequency ◽  
Recombinant Plasmid ◽  
Frequency Transformation ◽  
High Frequency Transformation

scholarly journals High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs bytrans-complementation ofSchizosaccharomyces pombe

1990 ◽  
Vol 18 (22) ◽  
pp. 6485-6489 ◽  
Author(s):  
Koei Okazaki ◽  
Noriko Okazaki ◽  
Kazuhiko Kume ◽  
Shigeki Jinno ◽  
Koichi Tanaka ◽  
...  
Keyword(s):  
High Frequency ◽  
Transformation Method ◽  
Frequency Transformation ◽  
High Frequency Transformation

High-frequency transformation ofArabidopsis thaliana byAgrobacterium tumefaciens

1992 ◽  
Vol 10 (2) ◽  
pp. 178-189 ◽  
Author(s):  
Michael C. Clarke ◽  
Wenbin Wei ◽  
Keith Lindsey
Keyword(s):  
High Frequency ◽  
Frequency Transformation ◽  
Ofarabidopsis Thaliana ◽  
High Frequency Transformation
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