Isolation of Corynebacterium freneyi from a case of exudative pharyngitis, a close mimicker of Corynebacterium diphtheriae

Corynebacterium freneyi is a recently described coryneform bacteria. It is only rarely identified from clinical specimens and its pathogenic significance has not been well studied. Here we report the isolation of the species from the throat swab of a patient with suspected diphtheria. The morphology on direct microscopy and culture also closely resembled Corynebacterium diphtheriae , which almost led to misidentification. The prompt clinical and microbiological response suggests a probable pathogenic role. This is the first report of the isolation of this species from an oropharyngeal sample.

RARE CAUSE OF BACTERAEMIA: MICROBACTERIUM ARBORESCENS.

Microbacterium arborescens, among coryneform bacteria are gram positive rods with orange pigmented colonies. Usually these are environmental contaminant but has shown invasive potential in immunocompromised individuals.A 56 yrs old lady presented with uncontrolled hyperglycaemia and hypertension. M. arborescens was isolated on blood culture. Identication was done based on biochemical characterictics and conrmed by Matrix assisted light desorption ionisation Time of Flight Mass Spectrometry( MALDI TOFF MS). Further amplication and sequencing was done and phylogenetic tree was contructed. Tree clearly shows clinical strain PGI 2 is different from environmental strain

Distribution of Epsilon-Polylysine Synthetases in Coryneform Bacteria Isolated from Cheese and Human Skin

ABSTRACTEpsilon-polylysine (ε-PL) is an antimicrobial commercially produced by Streptomyces fermentation and widely used in Asian countries for food preservation. Here we discovered a gene from cheese bacterium Corynebacterium variabile that showed high similarity to the ε-PL synthetase from Streptomyces in terms of enzymatic domain architecture and gene context. By cloning it into Streptomyces coelicolor with a Streptomyces albulus ε-PL synthetase promoter, we confirmed that its product is indeed ε-PL. A comprehensive sequence analysis suggests that ε-PL synthetases are widely spread among coryneform bacteria isolated from cheese and human skin; 14 out of 15 Brevibacterium isolates and 10 out of 12 Corynebacterium isolates contain Pls gene. This discovery raises the possibility that ε-PL as a bioactive secondary metabolite might be produced and play a role in the cheese and skin ecosystems.IMPORTANCEEvery year, microbial contamination causes billions of tons of food wasted and millions of cases of foodborne illness. ε-PL is an excellent food preservative as it is potent, wide spectrum and is heat stable and biodegradable. It has not been accepted by all countries (e.g those in the EU) partially because it was not a natural composition of food but rather originated from the soil bacteria Streptomyces, a famous producer of various antibiotic drugs and toxins. The unexpected finding of ε-PL synthetases in cheese and skin bacteria suggests that ε-PL may naturally exist in cheese and on our skin.

Granulomatous lobular mastitis associated with ductal carcinoma in situ of the breast

The association of granulomatous lobular mastitis and carcinoma of the breast is very infrequent. We present the case of a 44-year-old woman with concurrent granulomatous lobular mastitis with coryneform bacteria and ductal carcinoma in situ in the same breast.

Coryneform Bacteria in Granulomatous Lobular Mastitis: Morphological Diagnosis in Breast Biopsies

Granulomatous lobular mastitis is a rare disease whose origin is still unknown and shows an increase in its frequency. Morphological, microbiological, and molecular biology studies have linked this disease to lipophilic and fastidious corynebacteria, suggesting its possible infectious etiology. This series describes and reviews in detail the distinctive morphological characteristics of the bacteria present in the granulomas of this disease, the usefulness of histochemical techniques for their identification, and our proposal for a tissue quantification score for the bacteria. The MacCallum-Goodpasture method of Gram’s stain turned out to be the gold standard for examination, but we also highlight the efficiency of hematoxylin and eosin stain when it is exhaustively examined as well as the Grocott stain to evaluate the bacterial pleomorphism method, which is often underutilized.

Importance of coryneform bacteria in infective endocarditis

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